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To observe whether the re-expression of NAG7 gene could affect the gene expression of HNE1 cells, the cDNA microarray technique was employed to analyze the changes of gene expressions.

同时，运用cDNA微阵列技术分析NAG7基因重表达对HNE1细胞基因表达谱的影响。

Gene drugs ; gene therapy ; gene vaccine ; antisense RNA ; triplex-forming DNA

基因药物；基因治疗；基因疫苗；反义RNA ；三链DNA

Used the method of touch down PCR, the ACS2 gene was cloned from DNA of Petunia hybrida. At the same time, its gene sequence DQ090003 was obtained in GenBank, the antisense repetition of ACS2 linked the plasmid pBI-121 by use of one step. In the result, the expression vector having antisense repetition gene was constucted.

利用降落PCR的方法，从矮牵牛DNA中扩增出ACS2基因片段，在GenBank中获得基因序列号为DQ090003；利用一步法将ACS2基因的反义重复与pBI-121质粒连接，构建含该基因的反义重复表达载体。

FHIT gene alterations preferentially occur in squamous cell carcinoma patients and smokers, and FHIT gene may be a candidate molecular target of carcinogenesis in tobacco smoker. Allelic deletion of FHIT gene might be an early molecular event in smoking-related lung cancer.

FHIT等位基因缺失主要发生在肺鳞癌和吸烟患者中，且FHIT基因可能为烟草致肺癌的靶基因，其等位基因缺失可能是肺癌的早期分子事件。

Lines transformed by particle bombardment have been discussed. The selectable marker bar gene cassette that integrated with the rice genome had multiple copies and showed complex segregation behavsensitive plants in their progenies. In five out of ten original transgenic lines, bar gene can be stably transmitted as a dominant gene to self-pollinated T2 progeny.

结果发现：作为筛选标记的bar基因表达盒在水稻基因组中多拷贝整合，遗传分离行为复杂，还出现了Basta抗感分离比在35∶1～144∶1之间的&假纯合体&，但50﹪转基因株系中(5/10)bar基因可作为一个显性基因按孟德尔方式稳定遗传至自交T2代。

Lines transformed by particle bombardment have been discussed. The selectable marker bar gene cassette that integrated with the rice genome had multiple copies and showed complex segregation behaviors including the presence of 'false homozygotes', with abnormal segregation ratios ranging from 35:1 to 144:1 (Basta-resistant: sensitive plants) in their progenies. In five out of ten original transgenic lines, bar gene can be stably transmitted as a dominant gene to self-pollinated T2 progeny.

结果发现：作为筛选标记的bar基因表达盒在水稻基因组中多拷贝整合，遗传分离行为复杂，还出现了Basta抗感分离比在35：1～144：1之间的&假纯合体&，但50%转基因株系中（5/10）bar 基因可作为一个显性基因按孟德尔方式稳定遗传至自交T2代。

At the same time, gene interaction was observed in some of characters, transgressive segregation gene interaction was observed in polygenes controlling length of axial root and thickness of root base （ 1cm）, complementary gene interaction was significantly detected in nodule number of lateral root and dry weight of lateral root and nodule number of lateral root, but not detected or significant in the others.

控制主根长性状的多基因间存在互作，互作方式为重叠作用；控制主根粗（3cm）性状的基因间也存在一定的重叠作用，但是作用不明显；控制其他性状的基因都存在互作，表现为互补作用，但互补作用的强弱有差异。

Viciae hurL gene formed nodules in wild-type amounts, indicating that hurL gene is required for nodulation. Although the function of hurL gene in inducing nodulation remains unknown, the results of this work revealed that in addition to the nod and exo genes located on Sym plasmid, the R.

虽然目前对hurL基因影响豌豆根瘤菌诱导植物结瘤能力的机制并不清楚，但本文的研(来源：Acf6fBf6C论文网www.abclunwen.com)究结果首次证明：除位于共生质粒上的结瘤基因和胞外多糖基因外，豌豆根瘤菌在宿主植物上的结瘤还受到染色体基因hurL的控制。

The DNA solution prepared using this method has pH values ranging from 8 to 9, which can serve as PCR template directly for the amplification of genes like ribosomal RNA gene, mitochondrial cytochrome oxidase gene and chloroplast rbcL gene.

使用该方法制备的模板DNA溶液pH值在8到9之间，可直接用于核糖体RNA、叶绿体rbcL和线粒体COI等基因的PCR扩增。

It can be deduced that the similar P450 gene responsible for pyrethroid resistance is also existed in houseflies in Beijing district.But the same gene in the two districts have some differences in their P450 gene structure.

Hinf I酶切发现扩增产物上并没有相应的酶切位点，说明扩增产物与原序列MDCYP6D1的相应部分不一样，提示这一用PCR方法在北京地区的家蝇中扩增出的P450基因片段有可能对应一新的P450基因。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。