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The results indicated that the c-terminal fragment of Arabidopsis CRY1 gene was successfully integrated into the rape genome, mainly in the form of tabling, with a few in the form of single copy. The transformed gene could be stablytransmitted in the progeny, with a transmitted line rate of 62.5%. The genetic pattern of the transformed gene in the T_2 generation was mostly non-Mendelian. The transgene was expressed in some of the transgenic plants, but kept silent, in other plants.

结果表明，转化的外源目的基因多数以嵌合形式，少数以单拷贝形式整合到转基因甘蓝型油菜的基因组中；目的基因能稳定地遗传给后代，株系间遗传传递率为62.5％；T_2代的遗传行为少数呈现孟德尔遗传，多数呈现非孟德尔遗传现象；拟南芥CRY1基因能在转基因油菜中表达，但也出现了沉默现象。

Results: MMP2 gene expression could be detected in norma l subject. MMP2 gene expression levels in patients with chronic hepa titis and liver cirrhosis were higher than that of normal control. Th e gene expression level in patients with chronic hepatitis was higher in comparison with that in patients with liver cirrhosis.

结果：正常人有明胶酶A(MMP2)的基因表达；慢性肝炎、肝硬化患者MMP2基因表达较正常对照明显增强；慢性肝炎患者MMP2基因表达水平明显高于肝硬化患者。

The stage which is most sensitive to high temperature is trachea colouring embryo(ji-3), while the stage sensitive to dry condition is body pigmentation embryo(ji-5), so these two development stages have been called high temperature sensitivity stage and dry condition sensitivity stage respectively. The gene of high temperature and dry condition sensitivity is defined as sex-linked temperature sensitivity gene, which exists in 21.5 location of Z chromosome linking closely to sch gene, and presents concealed heredity.

其中对高温最敏感的时期是气管显现期(己_3)，对干燥最敏感的时期是转青期(己_5)，这两个时期分别称为高温敏感期和干燥敏感期，高温干燥敏感性基因定义为伴性温敏基因，t与Z染色体上21.5位置的sch基因紧密连锁，呈隐性伴性遗传。

A/DK/Hubei/216/83(H7N8) shared 96%～98%homology with different subtypes avian influenza viruses which isolated from HongKong during 1972～1997, in addition, evolutionary analysis showed that the remainingfive gene except NA and PB2, had close phylogenetic relationship with 1972～1997strains. A/DK/Hubei/137/83(H10N4), sequence similarity analysis revealed that theNS, PA, PB1, PB2 gene shared 98%～99% homology with the 1997～2004 H5N1subtype avian influenza virus strains, and except NA and M gene, the other five geneformed independence branch with their own, and the distance between the otherisolates were far.

前者HA氨基酸剪切位点序列为P-E-V-V-Q-G-R，后者HA剪切位点序列为P-E-I-P-K-R；2株病毒的NA在第48位氨基酸后无20个氨基酸的缺失；NS蛋白的第79～84位也没有氨基酸的缺失，不具有高致病性特征；A／DK／Hubei／216／83(H7N8)与香港1972～1997年不同亚型AIV的同源性为96％～98％，进化分析表明，除NA和PB2独自成一支外，其余5个基因都与1972～1997年毒株亲缘关系较近。A／DK／Hubei／137／83(H10N4)除NA和M基因外，其他5个基因分别独立自成一支。

To investigate the methods to effectively and simply assess the CAG repeat size of HD gene which was necessary for gene diagnosis of Huntington disease, the sequence including polymorphic CAG repeat of HD gene was amplified by PCR with TaKaRa LA Taq DNA polymerase and GC buffer. PCR products were analyzed on polyacrylamide gel to distinguish normal alleles from HD alleles. The DNA fragments of affected alleles were recovered from polyacrylamide gel as templets for secondary PCR. The secondary PCR products were cloned into T vector for sequencing analysis to determine CAG repeat size. A total of 20 normal individuals and 3 members from a HD pedigree were included in this study.

为了简单高效检测HD基因开放阅读框5'端n三核苷酸重复序列，建立快速准确的亨廷顿病(Huntington disease， HD)基因诊断方法，应用TaKaRa LA Taq DNA聚合酶配合GC buffer扩增HD基因包含n重复序列的目的片段，非变性聚丙烯酰胺凝胶电泳检测后回收n拷贝数异常增多的目的片段，再次PCR扩增后将产物连接至T载体，进行DNA测序确定CAG的拷贝数。

The genetic study suggested that the carbendazim-resistance in these mutants could be steadily inherited by asexual and selfed reproduction, and the resistance was controlled by the same single major gene both in laboratory mutants and field resistant isolates, the different levels of resistance or the same resistant level in different strains maybe conferred by mutations at different sites or one site with different allelic mutations. The gene compensates for the sensitivity to MDPC was allelic to that governs the carbendazim resistance, but the mutation for increasing sensitivity to MDPC in this gene could get the pathogen highly resistant to carbendazim.

抗药性遗传研究表明，在所研究的抗药突变体中，抗药性在自交和无性繁殖后代中能稳定遗传；室內抗药突变体和田间抗药菌株对多菌灵的抗药性由同一个主效基因控制，但它们发生突变的位点不同或者同一碱基位点发生了不同的突变；对MDPC的敏感性也是由单个基因控制的，该基因与控制多菌灵抗性的基因是等位基因，当该基因发生对MDPC的敏感性增加的突变时会使病菌对多菌灵产生高水平抗性。

In order to elucidate the relationship between the structural features of leginsulin gene in legume plants and their phylogenetic significance,we have cloned the cDNA sequence of leginsulin gene from radicles of broad bean via RT-PCR techniques according to the leginsulin gene sequence we previously obtained from soybean.The cloned cDNA encoded for a precursor protein consisting of the signal peptide,mature leginsulin and an additional 45 amino acids of another polypeptide.

为探明豆科植物中豆类胰岛素基因的结构特征与进化关系，在已获得大豆豆类胰岛素基因的基础上，以蚕豆种子胚根mRNA为材料，采用RT-PCR技术，克隆了蚕豆豆类胰岛素基因的cDNA序列，编码的前体多肽包括信号肽、成熟型豆类胰岛素及另一多肽的45个氨基酸残基。

In order to elucidate the regulation mechanism of RU5 region to BFV gene expression, BFV3026 provirus DNA was used to construct the plasmids containing different deletion of R region, which were cotransfected with luc report gene locatied behind the IP promoter to BL12 cells, and luciferase activities was assayed, confirming that U5 region could repress the initiation function of LTR as well as IP. The BFV structure genes with different deletion of R region were cloned closely behind to heterogenous CMV promoter, then transfected to 293T cells, RT activity was performed, testifying the R region was required for BFV pol gene expression, and also the function domain was identified within the 100n.t. sequence at the 5′end.

以牛泡沫病毒(Bovine foamy virus， BFV)中国株BFV3026原病毒DNA为材料，构建R区系列缺失质粒，通过对其转染细胞中RT水平及对缺失质粒与luc报告质粒共转染细胞中萤火虫荧光素酶活性的测定，确立U5区对于BFV3026两类启动子LTR和IP均具有负调控作用；同时将带有不同R区的BFV3026结构基因片段克隆于异源启动子CMV之下，通过对其转染细胞293T中RT酶活性的测定，确立R区对于病毒结构基因pol的表达具有一定的调节作用，并将其功能区域初步界定在R区5′端100bp内。

Besides characterisation of the two genes' function, RT-PCR, Northern analysis, primer extension, and lacZ reporter gene fusions were also tried in this study to investigate the genetic organization of the operons related and their transcription regulation, map gene was found to locate at the end of a long ribosome protein genes operon and yflG is in another operon with its downstream gene.

除了对这两个基因的功能鉴定，本文还阐明了它们各自所在操纵子的遗传结构，并采用RT-PCR、Northern印迹及引物延伸等多种方法，最终分别对它们的启动子进行了定位。

Coli lacZ gene at icp34.5 gene (RL1), an antiapoptosis and neurovirulence gene, which was constructed in our lab, is one of the oncolytic viruses.

本室构建的缺失凋亡抑制基因icp34.5并插入LacZ基因的单纯疱疹病毒就是一种有靶向性的溶瘤病毒。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。