查询词典 gene

Part three: An experiment study on the repair of peripheral nerve injury by NT-3 gene modified neural stem cells objective To investigate the effective treatment of peripheral nerve injury with NT-3 gene modified neural stem cells, to explore the feasibility of the treatment by means of gene transfection Methods 54 SD rats were divided evenly into 3 groups. The sciatic nerve transected model were made and sutured end to end with epineurium. The neural stem cell without NT-3 gene or with NT-3 gene modified were injected into triceps muscle one time a week, saline injected as control group. The nerve trunks of stomas and triceps muscle were acted as specimen three weeks、six weeks and nine weeks after operation.

最后，为了探讨神经营养素-3基因修饰的神经干细胞对周围神经再生修复的影响，从转基因角度探讨治疗周围神经损伤的有效方法，我们选用54只SD大鼠随机分为3组，造成坐骨神经切断损伤模型，神经外膜端端对位缝合后，于小腿三头肌每周分别注射生理盐水、未被神经营养素-3基因修饰的神经干细胞、及神经营养素-3基因修饰的神经干细胞。

The liqase was positively controlled by HAI and AI-2 to some extent. The lecithinase was negatively controlled by HAI-1, and the hemolysin was negatively controlled by both HAI-1 and AI-2. Special primers of luxS gene were designed from the luxS gene sequence of V. harveyi obtained from the GenBank, and the gene was amplified from a pathogenic strain V. harveyi VIB645 by PCR. The luxS gene was cloned to a cloning vector pUC, and was sequenced. It was found that the open reading frame is 519 bp. The similarity of the luxS gene from V. harveyi VIB645 to the published sequence of V. harveyi strain in GenBank was only 90%.

从GenBank中获取哈维氏弧菌中编码AI-2合成酶的luxS基因序列设计一对特异性引物从哈维氏弧菌VIB645中PCR扩增luxS基因，将luxS基因克隆于pUC载体并测序，发现其与GenBank中已发表的哈维氏弧菌的luxS基因序列相似性为90%，证明LuxS/AI-2系统在哈维氏弧菌不同的菌株中的具有一定的特异性，推测LuxS/AI-2系统可能与哈维氏弧菌的致病性或发光现象有关。

In order to reduce calculation error, the frequency distribution of average values is used to compute the mixed distribution's digital features of each component distribution, thereinto, the number of the component distribution is determined by AIC, choose the number that meets the minimum value of AIC as the component number of mixed distribution, and the other parameters are estimated by EM algorithm; Secondly, because each component distribution is corresponding to a kind of major gene genotype, according to the values of the average and variance of the each component distribution, we can use the limit error of the normal distribution to plot each individual into the correspondent component distribution, namely into correspondent major gene genotype. Then we regard each major gene genotype as a treatment level of one-way analysis of variances, and the one-way multivariate analysis of variance is carried out to calculate the covariance matrix of major gene effect, covariance matrix of polygene effect, covariance matrix of environment effect and so on; At last, combining the weights of the each component distribution of mixed distribution, we can calculate the variance of major gene effect, the variance of polygene effect, environmental variance and the genetic gain of the quantitative trait.

为减小计算误差，本研究采用均值的频数分布来计算各成分分布的数字特征，其中成分分布个数根据AIC准则，选择使AIC值达到最小的成分分布个数作为混合分布的成分分布数，分布中其它参数的确定利用EM算法来估计；其次，每个成分分布对应一种主基因基因型，根据各个成分分布的均值和方差，利用正态分布的极限误差将每个个体划分到相应的成分分布中，即相应的主基因基因型中，将每种主基因型作为单因素方差分析的一个处理水平，对其进行单因素的多元方差分析，分别计算主基因效应协方差阵、多基因效应协方差阵、环境协方差阵等参数；最后结合混合分布中各成分分布的权重即各主基因基因型的分离比例，计算主基因效应方差，多基因效应方差和环境方差，以及遗传力等参数，进而计算该数量性状的遗传进展。

Coli XL-1 strain, purposed cassette recombination plasmids pNB0098-K and pNB0097-K were constructed, in which the purposed gene was linked with plasmid pUC18, and the marker gene kanamycin was inserted in the purposed gene. The linear cassette DNA fragment was mixed in the medium of cultured competent cells of meningococcus BT878. The function of the purposed gene was inhibited by the homologous replace of casstte gene.

将线性的重组质粒片段置于培养基中，与感受态的脑膜炎双球菌混合培养，重组质粒中的同源性片段被脑膜炎双球菌细胞识别和摄取，整合到染色体上，目的基因被带有卡那霉素基因插入的同源性重组片段代替，导致失活，在选择性培养基上筛选出基因被敲除的突变株。

By retrovirus vector the foreign mdr1 gene could be efficiently transferred into bone marrow mononuclear cells of mouse in vitro, and it was confirmed that mdr1 gene was expressing stably and effectively in cells; in short terms there is no significant influence on the bone marrow MNC by transfection of mdr1 gene,but in the long run(in our study we had surveyed 2 months),there is a decline in the expression of apoptosis factor Bax in the MNC which transfected by mdr1 gene,in favour of the recovery of the receptor mouse;on the other hand it also poses a blance problem between multiplication and apoptosis in the process of receptor hematopoiesis after transplanting HSC which was transfected with mdr1 gene.

通过逆转录病毒载体可在体外将外源性mdr1基因转入小鼠骨髓单个核细胞中，转染的mdr1基因能整合到骨髓单个核细胞基因组中并有功能性表达；mdr1基因转入小鼠骨髓单个核细胞在移植后短期内对受体造血细胞没有显著影响，但从长期看来(本研究观察2个月)转染了mdr1基因的造血干细胞的凋亡因子Bax表达降低，更有利于受体鼠造血的恢复；另一个方面它也同时提出一个问题，即mdr1基因转染造血干细胞在回输受体后造血过程的增殖凋亡平衡。

It also can be used as alternative antigen of newgeneration vaccine.In this experiment,we screen the major protective antigen hsp65 gene of MAP in order to developnew vaccine especially the DNA vaccine for the prevention of paratuberculosis disease.The hsp65 genewas amplified from MAP C-2 chromosomal DNA by using the PCR technique.We gained a hsp65 gene of 1 626bp.Then PCR product was cloned into pGEM-T vector by T-A clone technique and therecombinant clone was identified by plasmid size,enzyme digestion and PCR identification.The cloneplasmid of pGEM-T- hsp65 was successfully constructed.The nucleotide sequence and deduced aminoacid sequence ofclone gene was analyzed by DNASTAR software.The result indicated that the size ofhsp65 gene consist with M.paratuberculosis K-10 strain in GenBank and the sequential homogeneityreached 99.1%,the amino acid homogeneity reached 99.3%.The preceding analysis indicated that thehsp65 gene was very conservative in M.paratuberculosis.

为了研发预防副结核病的新型疫苗尤其是DNA疫苗及相关蛋白功能，本研究选择了MAP的主要保护性抗原Hsp65蛋白，以副结核分枝杆菌C-2株染色体DNA为模板，以hsp65基因的特异性引物进行PCR扩增，获得了1 626bp的hsp65基因，通过T-A克隆技术，将PCR产物克隆至pGEM-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pGEM-T-hsp65，以DNASTAR软件分析了所克隆基因的核苷酸序列和推导的氨基酸序列，结果表明，所获得的hsp65基因与GenBank中MAPK-10株该基因核苷酸大小完全一致，两者核苷酸序列的同源性为99.1％，氨基酸序列的同源性为99.3％，表明该基因在副结核分枝杆菌中高度保守。

This gene contained 1 530 bp and encoded 510 amino acids, named IbMIPS-1. Sequence analysis indicated that IbMIPS-1 had high homology with Ricinus communis MIPS gene, Nicotiana tabacum MIPS gene, Sesamum indicum MIPS gene and Glycine max MIPS gene.

该基因由1530个碱基组成，编码510个氨基酸，与蓖麻、烟草、芝麻、大豆肌醇－1-磷酸合酶的氨基酸序列比对表明，其同源性很高。

The structural, functional and informational concept of gene is not completely unified at all levels, the overlapping gene in the structural concept of gene which will be likely to definite a gene in the functional and informational concept of gene.

基因的结构概念、功能概念、信息概念并非在各个层面上都是完全统一的，基因的结构概念中提到的重叠基因，在基因的功能和信息概念中有可能就将其定义为一个基因。

The frequency of losing exon 5-8 was the highest . It was consistent with the results of the FHIT gene whole length cDAN by RT-PCR.Conclusions: The above results showed that the changes of FHIT gene is probably related to the development of kinds of tumors, which is an important molecular event. FHIT gene can be considered to be a tumor suppresser gene, our studys provided a theoretic and experimental data for further research on the function of FHIT gene.

结论本研究结果初步证明FH工T基因改变可能与多种肿瘤的发生有关，是肿瘤发生的一个重要的早期分子事件，支持其作为一个抑癌基因在肿瘤发生中发挥作用，为进一步深入研究FH IT基因的功能提供了理论和实验依据。

In this experiment, His 5 gene was inserted into Ura 3 gene and constructed a new plasmid pLRH33, which contained a complete His 5 gene and partial Ura 3 gene at the ends of His 5 gene.

实验将His 5基因插入到一个Ura 3基因的中间，构建了一个新的质粒pLRH33，从而打断了Ura 3 基因使之不能表达。

The shaping method of noncircular part and the tool holder's radial motion characters in noncircular turning process are discussed in detail in the thesis.

论文详细研究了非圆零件的成型方法和加工过程中刀架的径向运动规律。

I have not really liked him,I do not like his this kind of disposition.

我没有真的喜欢他，我不喜欢他的这种性格。