查询词典 gene

Aim: The purpose of this study is to analyze if gene polymorphism of PPARγC161-T is an associated gene factor of the occurrence and development of coronary atherosclerosis through studing the association between gene polymorphism of PPARγC161-T and CHD and straitness degree of coronary artery.

但PPARγC161-T基因多态性与冠状动脉粥样硬化的发生、发展是否有关联目前尚无报导。

Results The hela cells which transfected with HSV-TK and IL-12 gene was obviously changed in appearance after 24 hours which added GCV, but the normal control cells growthed prosperity. The survival rate of the hela cells which transfected with HSV-TK and IL-12 gene was fall-off with the plus of tile GCV. The killing effect of GCV for the hela cells transfected with HSV-TK and IL-12 gene was more strengthener than that for the normal cells.

结果 加入GCV后体外培养联合基因转染的Hela细胞，24h后细胞明显发生形态上改变，正常对照组细胞生长旺盛；随着GCV剂量的加大，转染联合基因的Hela细胞的存活率呈逐渐下降的趋势，GCV对转染细胞的杀伤作用较对正常细胞的杀伤作用明显增强。

The three parts of this thesis involve gene regulation, gene structural analysis and novel gene cloning, respectively.

本文的三个部分分别涉及基因的表达调控、重要基因PLZF的结构分析，以及造血系统表达的新基因克隆。

Developmental biologists may be more emphasis on the informational concept of gene, evolutionary biologists may be more emphasis on the functional concept of gene, and molecular biologists may be more emphasis on the structural concept of gene.

发育生物学家可能会更偏重于基因的信息概念，进化生物学家可能会更偏重于基因的功能概念，分子生物学家可能会更偏重于基因的结构概念。

Effects of Adv-hBMP2 gene modify on the bone formation of tissue-engineered bone substitute. BMSCs of canine transferred by Adv-BMP2 gene expressed ALP in vitro and induced ectopic bone formation in nude mice. BMSCs adhered on the surface of FDB after 4hrs, expanded in 24hrs and proliferated in polylayer in 4d. FDB, combined with BMSCs transferred by Adv-BMP2 gene, induced bone formation in the subcutis of nude mice in 6 weeks.

结果：1.BMP-2基因修饰对组织工程化骨成骨的影响 BMSCs经Adv-BMP2基因转染后ALP染色强阳性、并在裸鼠肌肉形成异位骨组织；转基因的BMSCs与冻干骨复合后24小时即完全贴附生长，4天后复层生长并分泌大量胶原，植入裸鼠背部皮下6周后可见大量成骨，很少吸收，而单纯冻干骨或冻干骨复合BMSCs基本不成骨。2。

Objective To search and identify novel gene by screening the cDNA library of adult Taenia saginata asiatica,and to clone and express the gene so as to lay a foundation for the further study of Ta CRISP gene's function.

目的 通过筛选亚洲牛带绦虫成虫cDNA 质粒文库，识别半胱氨酸分泌蛋白，并进行克隆表达，为进一步研究其功能提供基础依据。

The cloned BBE gene sequences were 94.84% identified with the reported BBE genes in GenBank previously. Based on the cDNA sequences of COR and BBE ,two fragments about 400～500 bp from each gene with lower identity among them were cloned. The fusion gene BC (744 bp) is fused by the PCR technique. Then the promoter CaMV 35S driven, containing'forward BC fusion fragments-reverse pdk intron-reverse BC fusion fragments', plant siRNA expression vector were constructed based on the vectors pHANNIBAL and pART27.The work will lay the foundation for breeding a low morphine and high thebaine poppy.

利用blast及分子生物学软件DNAStar对COR和BBE基因的cDNA序列同源性进行分析比较，分别从各基因中筛选和克隆了一段同源性极低、约400～500 bp的片段；并应用重叠PCR法将其拼接成744 bp的融合基因BC，以中间载体pHANNIBAL和植物表达载体pART27为基础，构建了以CaMV 35S启动子驱动的含有"正向BC融合片段- pdk内含子-反向BC融合片段"的ihRNAi植物表达载体，通过转化野生罂粟，初步研究了以COR和BBE基因为靶标的RNAi对内源吗啡合成的抑制效果，为进一步培育低吗啡高蒂巴因的罂粟种质提供了依据。

Tube question grows which for the solution above standard, this research take mobile phone industry as a background, biological gene foreword generally unifies with the product tube, and the coordinate gene foreword compares to develops algorithm Basic local alignment search tool as well as transposed matrix Block substitution matrix the application, constructs the product gene tube system overhead construction, provides one same angle product tube way.

为解决上述色规范所衍生出的管问题，本研究以行动电话产业为背景，将生物基因序的概与产品管相结合，透过「产品特性」、「客户特性」、「订单特性」以及「色特性」等四大虚拟基因的定义，并配合基因序比对演算法 Basic local alignment search tool 以及置换矩阵 Block substitution matrix的应用，建产品基因管系统架构，提供一种同角的产品管方式。

Using semi-quantitative RT-PCR, the differential expression profiles of eleven selected genes were confirmed in the ovaries of triploid and diploid. These genes fell in gene categories with a wide range of functions. The results indicated that triploidy affects the dynamic gene regulatory network in triploid ovary. This study established a firm basis for future investigation on characterization of crucial molecular events for normal ovarian development in shrimp.To further dissect exact gene functions for gonad development of shrimp, three differentially expressed genes between diploid and triploid ovary, PCNA (proliferating cell nuclear antigen), CAS/CSE1 (cellular apoptosis susceptibility protein/chromosome segregation 1) and SSRF (spermatogonial stem-cell renewal factor) were characterized on certain aspects.

利用抑制性消减杂交技术，建立了对虾二倍体和三倍体卵巢间的2个消减文库；在正向消减文库(以三倍体卵巢作为实验组，二倍体卵巢作为驱动组)中，鉴定到54个基因；在反向消减文库(以二倍体卵巢为实验组，三倍体卵巢为驱动组)中，鉴定到16个基因；选取11个差异表达的基因，利用半定量RT-PCR的方法对其在二倍体和三倍体卵巢间的表达进行了检测，均能很好地与消减结果相吻合；这些差异基因编码多种功能的蛋白，分析表明染色体的三倍化使三倍体卵巢中的基因调控网络受到了影响；为深入揭示维持卵巢正常发育的关键分子调控事件奠定了基础。

Methods Amplify the cDNA sequence encoding truncated HCV gene, with a part of carboxyl-terminus deleted, by PCR. Synthesize the mimic epitope at E2 region of HCV and seven T or Th cell epitope genes of NS3-NS5 respectively. Bind HCV core gene with a part of carboxyl-terminus deleted to the synthetic epitope gene by PCR, then clone into eukaryotic expression vector pcDNA3.1 and transiently transfect COS7 cells.

用PCR方法扩增核心区羧基端部分缺失的基因片段；分别合成HCV E2区模拟表位和NS3~NS5 7个T或Th细胞表位基因；PCR方法将羧基端部分缺失的HCV核心区基因与合成的表位基因串联，克隆入真核表达载体pcDNA3.1，并通过脂质体瞬时转染COS7细胞。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48