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To analyse hereditary features and and rules of the foreign gene in the trans-Hu-IFN-α-gene carp,PCR testing technology were employed to test the Hu-IFN-α gene's genetic expression in its cross F1 and gynogenesis F1 generation.

为了分析外源基因在转基因鱼F1中的遗传特征和规律，采用PCR检测技术对Hu-IFN-α基因在转基因草鱼及其杂交F1，雌核发育F1中的遗传表达情况进行检测。

To analyse hereditary features and and rules of the foreign gene in the trans-Hu-IFN-α-gene carp, PCR testing technology were employed to test the Hu-IFN-α gene＇s genetic expression in its cross F1 and gynogenesis F1 generation.

文章摘要：为了分析外源基因在转基因鱼F1中的遗传特征和规律，采用PCR检测技术对Hu-IFN-α基因在转基因草鱼及其杂交F1，雌核发育F1中的遗传表达情况进行检测。

Three pairs of primers were designed according to nuc gene of SA, hly gene of LM and hemolysin gene of BC, and then the single PCR was performed to detect specificity and sensitivity.

根据SA的nuc基因、LM的hly基因和BC的hemolysin基因，设计合成三对特异性引物，然后分别进行单基因PCR反应的特异性和灵敏性验证。

Results The p53 gene mutation rate of colorectal cancer was 50%. The rate of apoptosis of tumor cells having mutation of p53 gene (22.11%) was much lower than that having no p53 mutation (40.57%, P＜0.05). Heteroploid cells had been found in 10/13 tumor samples with p53 gene mutation and 6/13 samples without p53 mutation (P＜0.05). The rate of apoptosis had no difference between the heteroploid cases and diploid cases.

结果 大肠癌标本p53基因突变率为50%；其中突变组肿瘤细胞凋亡率(22.11%)明显低于未突变组细胞凋亡率(40.57%)，(P＜0.05)；突变组异倍体细胞百分率(占76.9%)明显高于未突变组(占46.2%)，(P＜0.05)；异倍体肿瘤细胞凋亡率为34.0%，二倍体肿瘤细胞凋亡率为40.7%，二者差异无显著性(P＞0.05)。

Caghira gene is expressed in the unfertilized eggs and all stages of embryogenesis and the quantity of Caghira gene expression didn\'t changeobviously, which could be inferred that hira gene had an effect on gynogenetic development of gibel carp.

在银鲫的未受精卵中和胚胎发育不同阶段均可以检测到少量的转录产物，表达量未检测到明显变化，表明hira基因可能在银鲫雌核发育过程中发挥一定作用。

Caghira gene is expressed in the unfertilized eggs and all stages of embryogenesis and the quantity of Caghira gene expression didnt changeobviously, which could be inferred that hira gene had an effect on gynogenetic development of gibel carp.

在银鲫的未受精卵中和胚胎发育不同阶段均可以检测到少量的转录产物，表达量未检测到明显变化，表明hira基因可能在银鲫雌核发育过程中发挥一定作用。

The result of WISH showed that the trascription of hira gene distributed widely in the early stages of embryos development and centralized to head, heart and the end of tail. Hira gene might affect the development of brain and heart. The expression characterization of colored crucian carp Cahira gene in different tissues and different stages of embryogenesis were also investigated by RT-PCR.

胚胎原位杂交显示，Hira基因的转录本在银鲫胚胎早期广泛分布，而在胚胎后期主要集中于头部、心脏及尾芽尖部，说明也对其脑部和心脏的发育有影响。

It is suggests that Doc -1R gene has particular importance in maintaining the function of the tissues and Doc-1R gene perhaps is a house-keeping gene.

小鼠Doc-1R基因的mRNA在各组织均为阳性表达，因此提示，Doc-1R基因可能是一种对维持正常细胞功能具有重要意义的管家基因。

Forty-eight hours after transfection, cells were harvested to determine luciferase activity by illuminometer. In the presence of RU486 a 40-fold maximum activation of the luciferase reporter gene was observed in cultured cells, whereas in the absence of RU486, no significant activation was observed. There was a positive correlation between the luciferase activation and RU486 concentration in a definite range. The results showed that the RU486-inducible regulatory vector was successfully constructed, which can be used to regulate the expression level of genes of interest in appropriate time by the inducer RU486. This inducible expression vector provides a platform for the research of gene regulation and gene therapy.

加入诱导剂RU486后，可以诱导表达荧光素酶，并在一定范围内两者呈正比，最高可以实现荧光素酶的40余倍的表达，而没有RU486时，几乎没有报告基因的表达，表明RU486诱导调控载体构建成功，可实现对目的基因的表达时间和表达水平的精确调控，为进一步的基因调控研究和和基因治疗提供了良好的工具。

An enriched PCR fragments with less complexity were obtained and mainly expressed in the denervated hippoampus After cloning into the plasmid vector, 46 clones were initially screened and subjected to autosequencing By comparison with the EMBO gene databases, 21 gene fragments of different types were obtained with 11 similar to genes of known function and 10 similar to ones of unknown function It is inferable that the altered gene expressions of hippocampus following the entorhinally denervation may underlie the specfic reinnervation and the formation of synapses by transplanted entorhinal neurons with host neuropil within the denervated target areas.

初步挑选出的46个转化后的阳性克隆，经点杂交筛选和基因序列分析，与Gene Database同源性比较后，得到21种基因片段，其中功能已知的有11种，功能未知的有10种。结果表明海马在去内嗅皮层后某些基因表达的改变，这可能是移植胚胎内嗅皮层神经细胞轴突特异支配去神经靶区和重建突触联系有关。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48