查询词典 gene

Bioinformatics was used to seek the homologous gene of EXT1 in Strongylocentrotus purpuratus which was just discovered and had the correlation to human hereditary multiple exostoses. The sequence, exons, coding protein, physical and chemical characters of EXT1 gene were analyzed. The structure and function of its coding protein were predicted, and the phylogenetic tree for the homologous gene was constructed, which provided certain basis for the future research of human EXT1 gene.

利用生物信息学方法寻找人类遗传多发性外生性骨疣EXT1基因在紫色球海胆中的同源基因，对该基因的序列、外显子信息、编码蛋白及其理化性质进行分析，并预测其编码蛋白的结构与功能，构建其同源基因的系统进化树，旨在为进一步研究人体EXT1基因提供一定的依据。

This expression distribution suggests that COCH gene may contribute to maintain inner ear connective tissue extracellular matrix architecture, and do not support the postulation that gene might play an inner ear immune defensive function. 2. In vitro, the culturing spiral ligament type Ⅰ fibrocytes expressed COCH gene and POU3F4/brn4 gene, which were checked by RT-PCR and Western blot, and expression level did not chang with in 15 generations.

小鼠内耳COCH基因原位表达分布的观察结果提示，基因的功能与维持内耳间质组织细胞外基质的结构稳定有关，不支持COCH基因的功能与内耳免疫应答反应有关的推论。

The Principal componants analysis shows that the main gene of the red crane to habitat selection is the food, veil water and vegetation, for the white crane, food and veil are the main gene, for the white nape crane, the main gene are food ,water and vegetation, for white head crane are veil water and vegetation, however, food, veil and vegetation are the main gene for the common cranes habitat selection.

对五种鹤的主成分分析表明丹顶鹤对生境选择的主因子为食物因子、隐蔽因子、水因子和植被因子；白鹤对生境选择的主因子为食物因子和隐蔽因子；白枕鹤对生境选择的主因子为食物因子、水因子和植被因子；白头鹤对生境选择的主因子为隐蔽因子、水因子和植被因子；灰鹤对生境选择的主因子为取食适宜性因子、隐蔽因子和植被因子。

Total RNAs from KAx-3 cells and AK127 cells(developed for 14h) were isolated. After the reverse transcription and PCR reaction, two distinct differential fragments were acquired., fragment A was from KAx-3 cells and fragment C was from AK127 cells. After retriving and reamplifying the differentially expressed fragments, white-blue plaqueselection, the fragments were purified. Northern blot proved that fragment A was from KAx-3 cells and fragment C was from AK127 cells. The results of sequencing and researching for NCBI database have been showed: part sequence of fragment A shows 91% similarity to the gene encoding DhkA, 92% similarity to the gene encoding DhkF, 91% similarity to the gene encoding STATc, 97% similarity to the homoeobox gene encoding protein. These genes play important part in controlling cell differentiation and cell proportion in Dictyostelium discoideum.

本研究通过提取盘基网柄菌发育14小时的野生型KAx-3细胞和突变型AK127细胞的总RNA，运用mRNA差异显示法分离出了两条明显的差异表达片段，其中片段A来自野生型KAX-3细胞，片段C来自突变型Ak127细胞；并通过凝胶回收差异片段、对差异片段进行再次PCR、蓝白斑筛选克隆、提取质粒、酶切电泳纯化差异片段；接着进行Northern杂交的结果表明，片段A只与野生型KAx-3细胞的总RNA有杂交信号，片段C只与突变型AK127细胞的总RNA有杂交信号，这就排除了差异片段假阳性的可能；最后通过测序，搜索NCBI BLAST数据库发现：片段A的小部分序列与编码组氨酸激酶DhkA基因中一段序列的相似性高达91%，与编码组氨酸激酶DhkF基因中的一段序列相似性高达92%，与编码STATc蛋白基因的一段序列相似性达91%，以及与编码同源框蛋白的基因中的一段序列相似性达97%，这些基因在盘基网柄菌细胞分化和细胞比例调控过程中起着相当重要的作用，这些数据进一步说明了突变细胞不能完成发育的原因。

The summary results are below:1. GUS expression under the driving of the BjCHI1 promoter (-1060/+17) was essentially undetectable in the young seedlings under normal growth conditions. GUS activity was first detected in the stigma of young flowers, peaked in the young siliques, and decreased when the siliques became older. No GUS expression was found in the mature siliques, seeds or root.2. The BjCHI1 promoter (-1060/+17) was inducible by NaCl, PEG, wounding and MeJA treatments. High levels of GUS expression were detected in the transgenic tobacco and Arabidopsis plants after wounding, NaCl, PEG, and MeJA treatment, indicating that the BjCHI1 promoter responses to both biotic and abiotic stresses.3. RT-PCR analysis confirmed that the expression of the BjCHI1 gene in B. juncea was inducible by PEG and NaCl.4. The transcription start site was determined by 5′-RACE, and was located at the 17th nucleotide upstream of the translation initiation codon of the BjCHI1 gene.5. A -805/+17 promoter fragment was enough to response to wounding and MeJA induction, which was proved in transgenic tobacco and Arabidopsis plants. The 397 bp region between -805 and -409 of the BjCHI1 promoter contains a cis-acting element that is essential for the wounding and MeJA inducibility.6. The -695/-620 region was necessary but not sufficient to confer MeJA-responsive expression. A T/G-box locates in -353 play an important role in the expression of the BjCHI1 gene in response to MeJA treatment. The 76 bp region is coupled with the T/G-box to confer full MeJA-inducible transcription of the BjCHI1 gene.

主要结果如下：1、利用转基因拟南芥植株分析表明，正常生长条件下，BjCHI1启动子(-1060/+17)驱动GUS基因主要在花柱中表达，幼嫩的荚也有表达，并随着果荚的成熟而减弱，成熟的果荚、种子和根没有显示GUS活性。2、BjCHI1启动子(-1060/+17)能驱动GUS基因在转基因烟草和拟南芥中响应伤害的诱导，转基因拟南芥的分析还证明BjCHI1启动子也受MeJA、NaCl和PEG的诱导，证明BjCHI1启动子是一个伤害、MeJA、NaCl和PEG等生物和非生物因素诱导启动子。3、RT-PCR进一步证明芥菜中BjCHI1基因也受NaCl和PEG的诱导表达。4、5′-RACE法鉴定了BjCHI1启动子的转录起始位点，位于翻译起始位点ATG上游第17个碱基A.5、转基因烟草和拟南芥分析证明，-805/+17的启动子片段足以响应伤害和MeJA的诱导，-805和-409之间397 bp的启动子片段含有对伤害和MeJA诱导必要的元件。6、本明烟叶片瞬时表达系统分析证明，一段76 bp的序列(-695/-620)对BjCHI1启动子响应MeJA的诱导是必要的，但不足以响应MeJA的诱导，位于-353的T/G-box也参与MeJA的诱导。76 bp的序列(-695/-620)与T/G-box协同起作用，赋予BjCHI1启动子MeJA诱导性。

The pectin esterase gene and amino acid permease gene abundantly expressed in floral buds of NCa CMS while pectin esterase gene showed no transcript either in young leaves of NCa CMS or in the two tissues of maintainer line, and amino acid permease gene slightly expressed in the corresponding tissues.

结果表明，果胶甲酯酶基因和氨基酸通透酶基因都在不育系花蕾中大量表达，果胶甲酯酶基因在不育系叶片、保持系的花蕾、叶片中都没有检测到；而氨基酸通透酶基因有微量表达。

The gene therapy techniques for pancreatic cancer include antisense gene therapy, suicide gene therapy, immune gene therapy, and oncolytic virus therapy.

胰腺癌基因治疗的研究主要包括：反义基因治疗、自杀基因治疗、免疫基因治疗及肿瘤裂解病毒基因治疗等。

Viciae hurL gene formed nodules in wild-type amounts, indicating that hurL gene is required for nodulation. Although the function of hurL gene in inducing nodulation remains unknown, the results of this work revealed that in addition to the nod and exo genes located on Sym plasmid, the R. leguminosarum chromosomal hurL gene is also involved in controlling its capacity in inducing nodulation on host plant.

虽然目前对hurL基因影响豌豆根瘤菌诱导植物结瘤能力的机制并不清楚，但本文的研究结果首次证明：除位于共生质粒上的结瘤基因和胞外多糖基因外，豌豆根瘤菌在宿主植物上的结瘤还受到染色体基因hurL的控制。

Indica variety ZYQ8 is an important genetic resource resistant to rice blast disease in rice breeding in North China.in this study,resistance identification of the populations of F1,F2,DH and B1F1of the crosses of ZYQ8×Jingxi17 (JX17) and ZYQ8×Lijiangxintuanheigu was carried out by using a Chinese differential strainZH10-8-14 from North China and a Japanese differential strain Ken54-04 from Japan and the results demonstrated that ZYQ8 had one dominant major gene Pi-zh which had been reported by Zhu L.H., et al., The results from experiments in which the F2 populations of ZYQ8×nine different varieties with known resistant genes were inoculated with strain Ken54-04 indicated that the gene Pi-zh was a new gene and non-allelic to nine known gene loci,namely Pi-i,Pi-km,Pi-z,Pi-ta,Pi-ta2,Pi-zt,Pi-kp,Pi-b and Pi-t.

籼稻品种窄叶青8号是我国北方稻区水稻育种上重要的稻瘟病抗源之一。本文利用我国北方稻区的代表菌系中10-8-14和日本的代表菌系研54-04，对窄叶青8号与感病品种京系17号和丽江新团黑谷的杂交F1、F2、DH和B1F1群体进行抗病性鉴定。根据抗病性的分离，确认窄叶青8号的抗性由1对显性主效基因，即朱立煌等报道的Pi-zh基因控制。利用菌系研54-04接种窄叶青8号与9个具有已知抗病基因的鉴别品种杂交的F2群体，各群体都表现二基因的独立遗传，证明Pi-zh基因与Pi-i、Pi-km|、Pi-z、Pi-ta、Pi-taz、Pi-zt、Pi-kp、Pi-b和Pi-t等9个已知抗病基因间存在非等位关系，是新的抗稻瘟病基因。

The inhibinβA gene was studied as a candidate gene for the prolificacy of Jining Grey goats. According to the sequence of bovine INHBA gene, four pairs of primers were designed to detect single nucleotide polymorphisms of exon 1 and exon 2 of INHBA gene in both high fecundity breed and low fecundity breeds (Inner Mongolia Cashmere goat and Angora goat) by PCR-SSCP.

采用PCR-SSCP技术检测抑制素βA亚基基因（inhibin βA，INHBA）的编码区在高繁殖力山羊品种和低繁殖力山羊品种（内蒙古绒山羊、安哥拉山羊）中的单核苷酸多态性，同时研究该基因对济宁青山羊高繁殖力的影响。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48