查询词典 gene library

The gene for pyruvate kinase,which is involved in glycolysis and catalyze the biochemical reaction from phosphoenolpyruvate to pyruvate,was screened,cloned and sequenced from non photosynthetic Oomycetes,Achlya bisexualis cDNA library.

从真菌藻状菌纲Achlyabisexualis的cDNA文库中克隆了糖酵解途径的最后一种酶—丙酮酸激酶的基因，获得这个基因的完整阅读框架，并且对这个基因的全序列进行了测定。

It was reported that the pHZ1392 isolated from a genomic cosmid library of 10-22 might contain the complete gene cluster for 5102-Ⅱ bio-synthesis, which can inhibit some pathogenetic fungi, such as Cochliobolus heterostrophus and Piricularia oryzae, etc.

在过去的研究中曾报道10-22基因文库中的一个粘粒-pHZ1392可能含有完整的5102-Ⅱ抗生素合成基因簇，如果以pHZ1392转化变铅青链霉菌ZX64，可以使该宿主菌获得对玉米小斑病菌的抗性。

As Dr Davies reports in the Public Library of Science ,however, that is not what seems to have happened with at least one piscine antifreeze gene.

不过，正如戴维斯博士在《公共科学图书馆》期刊所报告的那样，进化并不只是发生在至少一种鱼类抗冻基因上。

In attempt to elucidate the molecular mechanism of cold tolerance of wintersweet, which blooms in the deep winter,a COR413 protein gene, designated as Cpcor413pm1, was obtained by sequencing the randomly selected clones, on the basis of Chimonanthus praecox flower cDNA library construction and its ESTs analysis.

为研究蜡梅冬季开花过程的抗寒分子机理，在构建蜡梅花cDNA文库及EST分析的基础上，通过随机克隆测序，克隆了1个蜡梅COR413蛋白的cDNA基因，命名为Cpcor413pm1。

To explore the gene which controls the rhizomatous development as well as other favorable genes, a cDNA library was constructed.

为了探索控制其地下茎发育的相关基因及其他优异基因，初步构建了地下茎cDNA文库。

A seed- specific expression gene (f128) was isolated from Setaria italica cDNA library.

从谷子 未成熟种子 cDNA 文库中克隆到 1 个种子特异表达的 f128 基因。

Na-K-Exchanging ATPase is a universal cell membrane protein in higher eukaryotic cells which mediates the anti-concentration gradient exchange of intracellular Na+ for extracellular K+. It plays essential metabolic roles such as maintaining sodium and potassium ion gradients across the plasma membrane. The targeted sequences were amplified by PCR to determine the genome structure of the hithertofore unsequenced portion of the alpha 1 subunit of the human Na-KExchanging ATPase gene which encodes 80-130 aa of its extracellular domain using two different templates, human genomic DNA and a human muscle cDNA library. The PCR products were analyzed by restriction endonuclease digestion and then cloned into a plasmid vector for chemiluminescence sequencing and further analysis.

Na-K-Exchanging ATPase是一种普遍存在于高等生物体内的细胞膜蛋白，主要参与介导K+和Na+在细胞内外之间的逆浓度梯度的转运，并维持一定细胞内外的离子梯度，我们采用聚合酶链式反应方法分别以人基因组DNA及cDNA文库为模板对人Na-K-Exchanging ATPaseα1亚单位基因胞外区约80-130位氨基酸编码序列进行扩增，限制性酶切分析扩增产物，并进行荧光测序，对测序结果进行同源性分析及剪接位点的搜索并对得到的核苷酸序列进行进一步的分析，发现人基因组DNA和cDNA经过扩增后分别得到833bp和195bp两种不同大小的片段Fg，Fc。

A human MDRl full length cDNA probe was used to screen a pufferfish cosmid library and nine clones, which would contain the whole MDR gene family in the pufferfish genome, were finally identified. One of the cosmid clones was sequenced. Sequence analysis revealed that there would be at least three MDR homologues in the corresponding region.

其表达产物为一跨膜蛋白p-glycoprotein，通过ATP提供能量，将进入细胞内的多种物质泵出细胞外，从而使细胞对这些物质产生耐药性，多药耐药基因的功能决定了它在肿瘤化疗中的位置而使其成为基因结构、功能和表达、调控研究中的热点。

ABSTRACT In order to screen chicken heart type fatty acid-binding protein gene genomic DNA library was constructed in λ phage.

本研究通过构建北京油鸡基因组DNA噬菌体文库，筛选含鸡心脏型脂肪酸结合蛋白基因的重组子，克隆出4株含鸡H-FABP基因片段的重组噬菌体。

Allen was a researcher at the Department of Neurobiology, The Babraham Institute, Cambridge, UK. Dr. Allen's research skills and techniques include DNA/RNA manipulation and purification/isolation, electrophoreses, PCR, cloning, all blottings, library screening, genetic mapping, design and construction of transgenes, artificial chromosome engineering, design and construction of vectors for gene targeting in ES cells, cell culture, histological techniques, mouse behavioural analysis, small animal surgery, and bioinformatic tools such as sequence databases and analysis tools.

从1996年到 2004年，Dr。 Allen 一直在英国剑桥Babraham 研究所神经生物学部门从事研究工作，包括DNA/RNA操纵、提纯/分离、电泳、PCR、克隆、印迹工作、文库筛选、遗传图绘制、转基因设计与构建、人工染色体工程、用于ES细胞基因打靶的载体设计与构建、细胞培养、切片技术、小鼠行为分析、小动物外科手术以及序列数据库和分析工具等生物信息工具的研究。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。