查询词典 gene library

objective to screen functional gene through construction of the cdna library of adult clonorchis sinensis.

目的 通过建立华支睾吸虫成虫cdna文库，筛选其功能基因，以研制华支睾吸虫的药物靶标。

Results cdc42-1ike protein gene was screened from the cdna library of adult clonorchis sinensis.

结果 从华支睾吸虫成虫cdna文库中筛选出cdc42样蛋白基因。

cdc42-1ike protein gene was screened from the cdna library of adult clonorchis sinensiswith bio-informatics method.

应用生物信息学方法从华支睾吸虫成虫cdna文库中筛选出cdc42样蛋白基因。

Methods The coding gene of Sj32KD antigen was amplified from the cDNA library of the adult parasite by PCR and cloned into the prokaryotic expression vector pET28a to construct a re- combinant plasmid Sj32-pET28a.

应用PCR方法克隆Sj32KD抗原基因，并在大肠杆菌系统中进行表达，利用His亲和层析方法纯化融合蛋白，并应用重组的Sj32KD抗原检测羊日本血吸虫病。

The GPA1 gene was obtained via PCR amplification and was cloned in the two-hybrid DNA binding domain vector pGBKT7 The combinant plasmid was designated as pGBKT7-Ga.β-galactosidase assay indicatedthat Got did not have the property of self-activation. After pGBKT7-Ga was transformed to yeast pJ69-4A, we transformed Arabidopsis vegetative tissue two-hybrid cDNA library plasmids to yeast pJ69-4A containing pGBKT7-Ga.

通过PCR扩增得到GPA1基因并将其克隆到双杂交DNA结合域载体pGBKT7中，得到的重组质粒命名为pGBKT7-Gα，β—半乳糖苷酶活性鉴定表明Gα不具有自激活特性，将其转化到酵母菌pJ69-4A中，再以此为受体菌转化拟南芥绿色营养组织cDNA文库质粒。

The full-length of cDNA in positive library plasmid pGADal was 1298bp. It encoded a polypeptide of 376 amino acids, which was just the same as the actin gene of Heliothis armigera.

为了确证HaNPV VP39与Actin的相互作用，将纯化的Actin蛋白和HaNPVVP39蛋白通过Western blot和等温量热滴定仪分析两者在体外的相互作用。

A GARP (glutamic acid-rich protein) gene was isolated from the macronuclear plasmid mini library of Euplotes octocarinatus .

一种富含GAA三核苷酸的 GARP (glutamic acid-rich protein)基因从八肋游仆虫大核文库中筛选获得。

Objective: On the basis of the random mutagenesis library of ARS, we wish to preparation by screening new ARS sequence which not only have higher ARS activity, but also display higher transformation frequency, in order to constructed new gene recombination vector which have higher efficiency in the Nonconventional yeast expression system.

目的：通过构建酵母ARS 随机突变体库，从中筛选出具有较强的转化效率的ARS序列，用以构建新型高效的克鲁维酵母基因工程载体。

The resulting strain, designated YES2MTM1, was transformed with a yeast genomic library. Transformants lost the plasmid overexpressing MTM1 after 5-FOA treatment. Yeast strains able to grow on nonfermentable carbon source with MTM1 deletion and overexpression of some DNA fragments were picked up and candidate suppressor genes were identified. Overexpression of five genes were identified to be able to rescue the growth defect on nonfermentable carbon source. The study will provide reference for MTM1 gene function and screening for suppressor of genes whose deletion result in irreversible damage.

为了避免MTM1缺失造成的不可逆损伤，在野生型酵母中先转入带有MTM1 基因的质粒，再敲除染色体上的MTM1 基因，随后转入基因组文库，再利用药物5-氟乳清酸(5-FOA)迫使细胞丢失表达MTM1基因的外源质粒，再筛选能在非发酵培养基上生长的转化子，通过这种方法筛选发现，POR2等5个基因的过表达可以挽救MTM1 基因缺失造成的非发酵培养基上的生长缺陷，为深入了解MTM1基因的功能提供了线索，对筛选其他造成不可逆损伤的突变基因的抑制基因提供了一条可行的研究思路。

Our strategy was constructing the cDNA expression library of the Oncosphere development stage by keeping away from the genome of Teania solium, and screening, cloning the immunogen genes, as well as expressing and analysis the interesting gene.

本研究避开庞大的基因组DNA，以构建六钩蚴发育阶段的cDNA表达文库为切入点，从中筛选、克隆免疫原基因，并进行表达和功能分析。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。