查询词典 gene library

Endosperm cDNA Library and Cloning Research of Phosphoenolpyruvate Carboxylase Gene

胚乳cDNA文库的构建及磷酸烯醇式丙酮酸羧化酶基因克隆的研究

Results A 462 bp length cDNA was amplified from S. japonicum cercaria cDNA library and identified to be RIRP gene cDNA by sequence analysis.

结果 从S 。japonicum尾蚴文库中扩增出一条长为 462bp的cDNA片段，经测序证实其为RIRP基因的全长cDNA。

Results showed that specific SIEA26~28 ku scFv with high level expression was achieved. The corresponding gene, ribosomal protein S4 was obtained by initially screening the cercaria cDNA library with the specific SIEA26~28 ku scFv. The obtaining of specific SIEA26~28 ku scFv lays the foundation for further screening and identification of the coding genes of SIEA26~28 ku, the natural molecular candidate vaccine against schistosomiasis.

结果显示，所获得的SIEA26~28ku特异性scFv，表达量高，采用该探针初步筛选出相关基因核糖体蛋白S4SIEA26~28ku特异性scFv的获得，为进一步筛选、分析鉴定抗日本血吸虫病天然分子候选疫苗SIEA26~28ku的编码基因奠定了基础。

Secondly, Dawkins introduced the idea of gene and body into the Library.

其次，道金斯把基因和肉体的理念引入到宇宙图书馆。

METHODS: Subtractive cDNA DNA library of PDLF was constructed with a recently developed gene cloning technique that is based on PCR and subtractive hybridization and then screened by restriction analysis and reverse southern dolt blot.

采用基于PCR和消减杂交的基因克隆技术构建体外培养的人PDLF与GF差异表达基因的扣除文库，采用酶切和反向杂交的方法筛选目的克隆，并进行测序和计算机分析。

Object To found a different cDNA library with Malasesiza furfur in hypha phase, and procure its gene fragment on differential expression.

目的：建立糠秕马拉色菌菌丝相细胞的差异表达cDNA文库，获得其差异表达的基因片段。

First, to isolate the total RNA both in hypha and yeast phase of Malasesiza furfur, Then reverse them to cDNA and found a different cDNA library with Malasesiza furfur in hypha phase by Subtractive Hybridization; At last, clone and sequencing the gene fragment on differential expression of Malasesiza furfur in hypha phase.

首先提取糠秕马拉色菌两相细胞的总RNA；然后逆转录生成两相细胞cDNA，运用消减杂交技术获得菌丝相细胞差异表达的cDNA，并建立差异文库：最后对差异cDNA片段克隆测序。

And a novel β-glucosidase gene was cloned and sequenced from this library.

从这个文库中筛选到一个新的β-葡萄糖苷酶基因。

Using gene chips to screen an SSH library could efficiently identify those differentially expressed genes without the need to examine previously cloned genes, and increase the number of target genes by decreasing the examination of multiple'house-keeping'genes.

因此，基于SSH方法和基因芯片技术的特点，将两者联合应用将是有针对性地发现大量差异表达基因的有效方法，而且可以大大提高发现新基因的机率。

Only one candidate gene is in the region, and the full-length ORF of SKC1 was obtained from the cDNA library of Nona by using PCR.

该区域内只有一个候选基因，利用PCR技术从Nona的cDNA文库中获得SKC1的全长ORF。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。