查询词典 gene library

In order to construct a normalized cDNA silencing library, a novel procedure was adopted to reduce the abundant copy number and increase the rare copy number. Then the normalized cDNA was put into pTRV vector to construct a cDNA library and this cDNA library was screened with the technology of virus induced gene silencing.

然后把均一化之后mRNA反转录得到cDNA，再与基因沉默载体pTRV重组，最后把构建好的载体通过电转化的方法转入到GV3101农杆菌中，从而建立起破色期番茄果实均一化cDNA沉默文库。

Carcinoma ; hepatocellular ; Gene library ; Augmenter of liver regeneration ; Interacting protein

癌； 肝细胞；基因文库；肝再生增强因子；相互作用蛋白

It has been widely used in analysis of gene expression map, analysis, discovey of new gene ,gene mutation and polymorphism analysis, genom library map, gene sequence, disease diagnosis and prevention, drug selection, tumor biology and environmental toxicology.

基因芯片技术目前已广泛应用于基因表达谱分析、新基因发现、基因突变及多态性分析、基因组文库作图、基因测序、疾病诊断和预防、药物筛选、药物开发和研究、肿瘤生物学、环境毒理学等研究领域。

So it couldn't fit dynamic network environments soundly. After investigating of negative selection, affinity mature and memory detector evolution mechanisms, it is Embedded Negative Selection Operator Clonal Selection Algorithm presented combined with negative selection, clonal selection and memory detector gene library evolution.

本文结合网络入侵检测的上述问题，深入研究了否定选择、亲和力成熟过程以及免疫记忆机制等，以现有克隆选择算法为主体，将否定选择、克隆选择、记忆检测器基因库方法融合进来，提出嵌入否定选择的克隆选择算法(Embeded Negative Selection Operator Clonal Selection Algorithm， ENCSA)。

The synthesized cDNA is a continuous band less than 6. 0kb shown on x-film through α〓PdCTP which was inserted in the first strain of cDNA. The titer of the Ha-cDNA library was about 1. 5×10〓cfu/ml which could represent all protein-encoding sequences from Hz-AM1 cells; Namely, the cDNA library has representativity. After the successful construction of the Ha-cDNA library, the open-reading frame of vp39 gene of Heliothis armigera was then fused in frame with the Ga14-DNA-binding domain in the pGBT9 plasmid and the fusion protein Ga14-VP39 was expressed in yeast to be used as bait protein.

在成功构建棉铃虫细胞Hz-AM1cDNA文库的基础上，本文接着将棉铃虫核型多角体病毒衣壳蛋白VP39（HaNPV-VP39）基因克隆到酵母双杂交系统(yeasttwo-hybrid system)中的质粒pGBT9上。pGBT9上含有酵母菌转录激活因子GAL4 DNA结合域的编码区（GAL4-DBD），使克隆到质粒pGBT9上的HaNPV-vp39基因与GAL4-DBD基因融合，在酵母菌中表达DBD-vp39融合蛋白，该融合蛋白即为酵母双杂交系统中的诱饵蛋白。

Objective To search and identify novel gene by screening the cDNA library of adult Taenia saginata asiatica,and to clone and express the gene so as to lay a foundation for the further study of Ta CRISP gene's function.

目的 通过筛选亚洲牛带绦虫成虫cDNA 质粒文库，识别半胱氨酸分泌蛋白，并进行克隆表达，为进一步研究其功能提供基础依据。

After thecomplete genome extraction of the strain was performed, the genomic DNA was partiallydigested by restriction enzyme Sau3AⅠ, the DNA fragments from 1 to 5Kb was clonedinto prokaryote expression vector pET-28a-c, and transformed host bacteria. The resultsshowed that we succeeded in constructing the gene expression library of haemophilusparasuis serovar 5, which is fundamental for the study of advanced gene screening. Inaddition, primer design was performed based on haemophilus influenzae in this study. In addition, PCR was performed by using genomic DNA of haemophilus parasuisserovar 5 as the template. The results demonstrated that we obtained two neo-gene:23SrRNA gene(conserved gene belonging to the large-subunit of ribosome) and adenylatecyclase gene(encodes adenylate cyclase and participates in converting adenyl nucleosidetriphosphate to cyclic adenosine3",5"-monophosphate). Furthermore, the phylogeneticanalyses between the species was performed, and neighbor-joining tree was constructedbased on comparison of 23S rRNA gene sequences, so it was illuminated betweenHaemophilus parasuis and other species in molecular evolution relationship.

选择我国流行优势菌株副猪嗜血杆菌血清5型地方株为研究对象，提取细菌基因组DNA，用限制性内切酶Sau3AⅠ对基因组DNA进行部分酶切，回收大小为1～5Kb的DNA片段，将其连接入原核表达载体pET-28a-c，最后转化宿主菌，结果成功地构建了基因表达文库，为后续的基因筛选工作奠定基础；另外，本研究选择嗜血杆菌属的流感嗜血杆菌为参考对象进行引物的设计，以副猪嗜血杆菌血清5型地方菌株的基因组DNA为模板，进行PCR扩增反应，结果表明成功地获得两个新基因：23S rRNA基因(存在于核糖体大亚基中的保守性基因)和腺苷酸环化酶基因(负责将腺嘌呤核苷三磷酸转变为环腺苷酸)，并进一步做了不同物种之间的分子系统发育分析，构建了基于23S rRNA基因的邻接法系统发育树，阐明了副猪嗜血杆菌与其它菌种的分子进化关系。

Arteries ; Heart ; Gene library ; Expressed sequence tags ; DNA ; comple mentary

动脉；心脏；基因文库；表达序列标签； DNA ；互补

New laccase gene and its full-length cDNA were clone from genomic library and equalized cDNA library of Ganoderma lucidum. Similarity between the sequence and other laccase gene were analyszed with NCBI blast, the results show that the new gene belong to multicopper oxidases gene family and have 60-70% Similarity with other laccase gene.

从灵芝的基因组文库和cDNA均一化文库中克隆了灵芝的漆酶基因和全长cDNA，并通过NCBI Conserved Domain Search氨基酸序列的相似性分析及与其它真菌漆酶之间的相似性分析，证明得到的蛋白是一种新的multicopper oxidases，其蛋白质序列与其它已克隆漆酶基因的同源性在6 0～7 0％之间，并对论文克隆的漆酶基因的启动子和终止子进行了分析。

Clarke-Carbon equation can give a good estimation for the necessary number of recombinants in a gene library.

Clarke-Carbon公式用于估计一个完整的基因文库所需的最少克隆数。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。