查询词典 gene flow

Methods: The expression of mutated P53 gene in human lymphocytes was investigated using flow cytometry.And the micronucleus and sister chromatid exchange tests were studied in vitro.

以正常人外周血淋巴细胞为材料，用流式细胞仪检测环磷酰胺对突变型P53基因表达的影响，同时以微核及姐妹染色单体互换为指标，进行致突变研究。

Cell growth and apoptosis were measured by MTT method and flow cytometry. TRAIL gene expression before and after combination with DOX was measured by RT-PCR.

将重组腺病毒载体介导的TRAIL基因作用于大肠癌细胞株RKO，并联合低剂量的阿霉素协同作用。

Cell growth and apoptosis were measured by MTT method and flow cytome-try. TRAIL gene expression before and after combination with DOX was measured by RT-PCR.

将重组腺病毒载体介导的TRAIL基因作用于大肠癌细胞株RKO，并联合低剂量的阿霉素协同作用。

Methods DNA clone of AD8 and NL43 was respectively transfected to skin by Gene Gun, The expression of virus in the epidermal cells was observed by flow-cytometry and fluoroscope.

以基因枪转染AD8及NL4 3分子克隆至皮肤，流式细胞仪和荧光显微镜观察病毒在表皮细胞中的表达，磁分离术分离CD80 +和CD80 -的游离细胞，进而以PCR和逆转录酶检测游走细胞中HIV对CD+ 4 T淋巴细胞的感染和表达。

After that, we detect cell DNA heteroploid and cell cycle transformation of human osteosarcoma tissue by using flow cytometry method; next, we amplification the exon2 and exon3 of 〓 gene in 36 cases human osteosarcoma tissue, and amplification products were performed the sepharose and polyacrylamide gel electrophoresis immediately.

然后采用原位杂交方法，对45例骨肉瘤标本组织和10例骨纤维结构不良组织中〓基因mRNA进行检测，以了解骨肉瘤标本组织和骨纤维结构不良组织中〓基因在蛋白质水平和mRNA水平的表达情况。

In this study, we found out that linalool, a small-molecule compound, could inhibit the growth of various human lymphocyte leukemia cell lines significantly. Meanwhile, it showed little toxicity to normal hematopoietic cells. To determine effects of linalool on the growth of a serious of human leukemia cell lines and normal hematopoietic cells and to explore its possible mechanisms, we employed various technologies, including MTT assay, Flow cytometry, Human Cancer PathwayFinder II Gene Array, Western Blot, RT-PCR and so on.

我们在筛选抗肿瘤小分子化合物时，发现天然小分子化合物芳樟醇能明显抑制多种人淋巴细胞白血病细胞增殖，而对正常人骨髓造血细胞及外周血细胞的增殖没有明显的影响，为此我们用MTT技术、流式细胞术、肿瘤相关信号通路分析技术以及Western blot、RT-PCR等手段和方法探讨了芳樟醇对不同类型白血病细胞系及相应正常造血细胞中的影响情况，及其诱导白血病细胞凋亡的可能分子机制。

Methods In vitro, human gastric cancer cell line SGC-7901 in log phase was intervened by As_2O_3 (final concentrations in order:1.0,5.0,10.0μmol/L) and 5-Fu (final concentration:50mg/L) respectively alone or in combination, and incubated for 24h、 48h、 72h. Blank group is set up. The cell apoptosis and cell cycle changes were observed by flow cytometry, and the expression of apoptosis-related gene Bcl-2 was analyzed by immunocytochemical method and absolute gray scales quantitative method.

将对数生长期的人胃癌细胞株SGC-7901(终浓度为2×10~5)经低、中、高三种浓度的As_2O(终浓度分别为1.0，5.0，10.0μmol/L)和5-Fu(终浓度50mg/L)单独及联合处理，设空白对照组，分别孵育24h、48h、72h后，收取标本采用流式细胞术测定细胞凋亡率及细胞周期变化；免疫组化绝对灰度定量分析检测凋亡抑制基因Bcl-2的表达。

To 7 days after transduction, cells were observed and recorded with fluorescence microscope, the expression levels of report gene EGFP in tumor cells were examined by using flow cytometry and Western blotting, the expression of report genes luciferase was analyzed with luminometer to obtain the relative light units.

用荧光显微镜观察EGFP阳性细胞，流式细胞仪检测EGFP阳性细胞的百分率和单个细胞平均荧光强度，Western印迹检测EGFP蛋白表达的变化以比较各组病毒转导效率。

Classification examination was put into practice for only normal cells observed.For the dubious specimen,tuberculosis-Ab,acid-fast bacteria staining,flow cytometry,CD series,gene,DNA aneuploidy,ZPP and tumor markers etc were detected. Results Tuberculosis lymph gland inflammation coverd highest rate:546 cases (36%) in tuberculosis node was 252 cases (46.2%),lymphonode period was 184 cases (33.7%),early tuberculosis lymphagland inflammation was 233 cases (44.7%).

利用改良一次性5～10ml注射器(22G、7号注射器，外径＜0.7mm)，针吸取材、涂片、行改良瑞-姬双染技术，低倍镜查找特殊异常细胞，油镜仔细观察细胞形态结构特征，对于未见异常细胞要进行分类检查，对疑难标本，同时做结核抗体、抗酸菌染色、流式细胞仪、CD系列、基因、DNA异倍体、锌原卟啉、肿瘤标志物等检测指标。

Classification examination was put into practice for only normal cells observed.For the dubious specimen,tuberculosis-Ab,acid-fast bacteria staining,flow cytometry,CD series,gene,DNA aneuploidy,ZPP and tumor markers etc were detected. Results Tuberculosis lymph gland inflammation coverd highest rate:546 cases (36%) in which tuberculosis node was 252 cases (46.2%),lymphonode period was 184 cases (33.7%),early tuberculosis lymphagland inflammation was 233 cases (44.7%).

利用改良一次性5～10ml注射器(22G、7号注射器，外径＜0.7mm)，针吸取材、涂片、行改良瑞-姬双染技术，低倍镜查找特殊异常细胞，油镜仔细观察细胞形态结构特征，对于未见异常细胞要进行分类检查，对疑难标本，同时做结核抗体、抗酸菌染色、流式细胞仪、CD系列、基因、DNA异倍体、锌原卟啉、肿瘤标志物等检测指标。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。