查询词典 gene base

A pair of primers based on conservative sequences of Drosophila melanogaster was designed to amplify the p53 gene. The fragment with 1156 base pair was obtained and sequenced. The percent identity between others' and ours' was 99.4%, with the other biological sequence of p53 existing some differences.

从紫外刺激的果绳抽提总RNA，通过RT-PCR方法扩增得到有完整读码框的p53基因，并且通过对p53基因的序列分析表明，所得到的序列与网上已经登陆的果蝇p53序列有99.4%的相似系数，而与其他生物的p53序列存在一定的分歧。

The sequencing result shows that this sequence is 1978 bp at full length. This means that the specific fragment could be used as a base to clone sex-linked gene of Spinacia oleracea L.

测序结果显示，该片段全长1 978 bp，该特异条带的获得为菠菜性别相关基因的克隆奠定了基础。

A basic knowledge of microbiology, will be detected with a microscope observation of micro-organisms, medium Preparation, disinfection and sterilization, separation and purification of micro-organisms, bacteria counting method A chemical analysis of the theoretical knowledge and can use chemical analysis methods and equipment of the components of material analysis Master fermentation process principles, understanding of beer, liquor, fermentation production technology Master protein related knowledge, PAGE gel electrophoresis technology, high-speed centrifugal Genetic engineering master the basic theory of knowledge, the extraction and purification of genes, PCR principle, gene recombination technology Master of the basic theoretical knowledge, the immobilized enzyme, the enzyme preparation Application Master of animal and plant tissue culture techniques of experimental steps studied experimental apparatus: Balance (scales, electronic scales, Analytical Balance), or acidity, spectrophotometer, liquid gas chromatography, acid-base titration apparatus, microscopes, centrifuges, ultrasonic cleaning, constant temperature oscillation incubator, super-clean table, steam sterilization pot, the pot constant temperature water bath, PAGE device apparatus, oven, fermenter, such as PCR instrument.

具备微生物理论基础知识，会用显微镜检测观察微生物，培养基的制备、消毒与灭菌，分离与纯化微生物，细菌的计数法具备分析化学的理论知识，能运用化学分析和仪器分析方法对物质的组分进行分析掌握发酵工艺原理，了解啤酒、白酒、发酵生产工艺掌握蛋白质相关知识，PAGE凝胶电泳技术，高速离心掌握基因工程的基本理论知识，基因的提取和纯化，PCR技术原理，基因重组技术掌握酶的基本理论知识，酶的固定化方法，酶制剂的应用掌握动植物组织培养技术的实验步骤学过的实验仪器：天平（台天平，电子天平，分析天平）、酸度计、分光光度计、液气相色谱仪，酸碱滴定仪器，显微镜，离心机，超声波清洗器、恒温振荡培养箱、超净工作台，蒸气灭菌锅、恒温水浴锅，PAGE电泳装置仪器、干燥箱、发酵罐、PCR扩增仪等。

The 433 base pair segments of the mitochondrial cytochrome oxidase I gene from 62 individuals of Frankliniella occidentalis and other 8 common thrips species in China were sequenced.

用PCR产物直接测序法对入侵害虫西花蓟马和其他8种蓟马的线粒体CO I基因433bp片段测序，获得62个个体的序列。

The 433 base pair segments of the mitochondrial cytochrome oxidase Ⅰ gene from 62 individuals of Frankliniella occidentalis and other 8 common thrips species in China were sequenced.

用PCR产物直接测序法对入侵害虫西花蓟马和其他8种蓟马的线粒体COⅠ基因433bp片段测序，获得62个个体的序列。

All theses studies established a base for transforming Acer negundo gene, had important value for cloning aceraceous and other plants.

为复叶槭的基因转化奠定了基础，对槭树科其他树种的无性繁殖和遗传改良具有重要参考价值。

From differentiation of soybean varieties and photoperiod adjustment, anatomical differentiation essence of different types of stem termination soybean varieties, variation law of stem termination development under different photoperiod treatments, effect of long day on the terminal raceme of soybean, regulation rules of AGAMOUS gene among different soybean varieties and under different photoperiod treatments were studied, which would provide theory base of plant type breeding of soybean especially improvement of podding-habits and cultivation and management of soybean.

因此，试验于2006和2007年在中国农业科学院作物科学研究所完成，主要从大豆的品种差异和光周期调控入手，利用解剖学和分子生物学技术，研究不同结荚习性大豆品种的本质差异、大豆在不同光周期处理下茎顶的变化规律，进一步明确长日照对大豆顶端花序的影响，及AGAMOUS基因在不同光周期处理下大豆品种中的表达规律，为大豆株型育种特别是结荚习性的改良及大豆栽培管理提供理论依据。

Because of sharing characteristics with both Galliform groups, Tragopans are classified as partridges by some authors and pheasants by some others. In this study we set out to sequence the cytochrome b-gene of 19 species pheasants. and took the sequence for those 22 species from GenBank. We amplified 828 base pairs using the PCR technique. Primers designed by ourselves were based upon Galliform sequence data. Neighbor-joining method and Minimum evolution method were used to analyse cytochrome b sequences with Anhima cornuta and Kachuga dhongoka as outgroups.

通过PCR扩增鸡形目Galliformes黄腹角雉Tragopan caboti等10个属19个个体的线粒体DNA细胞色素b的部分基因，获取片段长度为828bP，以及从GenBank获取22种样本的相应序列，以角叫鸭Anhima cornuta和海龟Kachuga dhongoka为外群，分别用邻接法(Neighbor-joining， NJ)和最小进化法(Minimum-evolution， ME)，对鸡形目和将雉科的15个属分为5个属及雉族和鹑族构建分子系统树，NJ和ME系统树中，都是角雉与雉类相聚。

To establish the base of the control of the Citrus aurantium L. fruit maturity in molecular level and produce high quality Fructus Aurantii, a pair of degenergte primers were designed according to the conserve regions of ACC Oxidase gene of many kinds of plants.

根据已报道的各种植物ACC氧化酶氨基酸序列上前后两个保守区设计1对简并引物，以酸橙叶片总RNA为模板，通过RT-PCR扩增到1个834bp的基因片段。

By separation and combination analyses of nuclear rDNA ITS and the cpDNA (matK gene, rpl16 intron and atpB-rbcL spacer) sequences, the interspecific relationships were discussed, and the classification review and biogeographic analysis were done for all species bearing raceme or spike of one to many umbels with prophylla at base.

运用nrDNA ITS及cpDNA(matK、rpl16、atpB-rbcL)序列的单独和联合分析探讨了这些类群的种间关系，并对世界菝葜属中具有一至多个伞形花序排列成圆锥花序或穗状花序、花序梗上有关节的所有种进行了分类学研究及生物地理学研究。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。