查询词典 gene base

We can make use of chip to analysis virus gene group; construct mutation detection technique of heredity diseases and become normal detection technique of many heredity diseases; supervise the changes of cell gene expression, research pathogenic theory of virus; differentiate bacteria gene types and identify bacteria strains, provide base data for screening and supervise drug-fast gene.

利用基因芯片可加速对病毒基因组的功能分析；可建立遗传病的突变检测技术，并成为对多种遗传病的常规诊断技术；可用来监测宿主细胞基因表达的改变，研究病毒的致病机理；基因芯片还可用来对细菌基因的分型和菌种鉴定，为筛选和监测细菌的抗药性基因提供基础数据。

We can make use of chip to analysis virus gene group; construct mutation detection technique of heredity diseases and become normal detection technique of many heredity diseases; supervise the changes of cell gene expression,research pathogenic theory of virus; differentiate bacteria gene types and identify bacteria strains,provide base data for screening and supervise drug-fast gene.

目前我国在出入境检验检疫中，已开始在基因芯片技术检测传染病病原方面开展了研究工作，国家质检总局动植物检疫所研制出了检测SARS病毒的全基因芯片，极大的促进了出入境检验检疫检测技术的发展，但目前国内外尚未见到有关新城疫病毒基因芯片的报道。

The fused insecticidal protein may delay the process of insects?developing resistance. After investigation of the secondary structure and action mode of Bt insecticidal protein and Cowpea trypsin proteinase inhibitor, as well as the action mechanism of insect enterokinase, we devised the fusion gene on the base of maintaining their functions respectively. The method follows: CpTI gene is linked to 3?-end of GFM crylA gene by a nucleotide sequence encoding cleavage site of insect enterokinase.

根据Bt杀虫蛋白Cry1A和豇豆胰蛋白酶抑制剂的高级结构、杀虫机理及昆虫体内肠激酶的作用机理，本着尽量不影响二者功能的原则，设计出了构建Cry1A和CpTI融合杀虫基因CryCI的方案，即将CpTI基因的编码序列连接到GFM Cry1A基因编码区3'端，并在其间用一段编码肠激酶切割位点的序列相连。

Repens L. and E. intermedia Nevski by using SSR, in order to provide science theories basis and base to further study E. repens L. drought resistance gene molecular marker, discover excellent drought resistance gene source , fast and efficiently select with the molecular marker of drought resistance gene catena for E.

通过对20 份来源不同的偃麦草与中间偃麦草种质材料在室内模拟逆境和田间干旱条件下进行抗旱材料的筛选和鉴定，应用SSR 技术对偃麦草抗旱分子标记进行引物筛选，为进一步深入研究偃麦草与中间偃麦草抗旱基因分子标记、发掘优异抗旱基因源，快速高效筛选出与偃麦草与中间偃麦草抗旱基因连锁的分子标记提供科学理论依据和基础。

The DNA fingerprints of 4 biotypes of GM population from 7 locations were analyses by AFLP.Base on the fine mapping of resistance gene Gm6 against all 4 Chinese GM biotypes by RAPD and SSR methods respectively,the STS markers and SSR markers linked to gene Gm6 were used for breeding through marker assisted selection.Some new resistant germplasm with Gm6 gene were created.And 6 cultivars and 6 lines of two-line hybrid rice and 1 line of three-line hybrid rice against GM were bred and extended to the farmer.The technique system of MAS for resistant varieties against Asian rice gall midge was set up at Guangdong province of China.

用AFLP方法对从中国广东省7个地点采集的4个生物型的DAN指纹进行分析；在对用RAPD和SSR技术分别对抗中国4个稻瘿蚊生物型的基因Gm6精细定位基础上，用与Gm6紧密连锁的STS和SSR标记开展分子标记辅助育种，创造了一批抗稻瘿蚊的新种质，包括育成了6个栽培稻和6个二系杂交稻和1个三系杂交稻并在农户试种，在中国广东成功地建立了分子标记辅助选育抗稻瘿蚊品种的技术体系。

The cDNA document of ACC oxidase gene of Citrus aurantium L. was cloned. By using the program of BLAST on NCBI GenBank database, the sequence presented a very high match with the ACC oxidase genes from other plants. The base sequence was analysed by using biology programe of DNAStar 5.0. 278 amino acids were coded by the base sequence and the base sequence had the same conserve region of ACC oxidase gene of many kinds of other plants. The base sequences comparability was more than 72% compared with those of many kinds of other plants. The amino acid sequences comparability was more than 70% compared with those of a lot of other plants.

克隆了酸橙1-氨基环丙烷－1-羟酸氧化酶基因cDNA片段，将片段序列在NCBI网站上进行同源性搜索，显示的皆为不同植物的ACC氧化酶基因，因而认为所克隆的片段就是酸橙ACC氧化酶基因；并运用DNAStar5.0软件进行序列分析，推导的氨基酸序列为278个残基；具有所有植物ACC氧化酶基因共有的保守区域；与多种植物的ACC氧化酶基因的核苷酸和氨基酸序列的同源性都在72%和70%以上。

They all contain 13 protein coding gene,2 rRNA gene,22 tRNA gene and 1 D-Loop.The base composition for the four nucleotides is A-32.0%,C-27.6%,G-14.7%,T-25.8%,And it is A-32.5%,C-26.9%,G-14.1%,T-26.5%for Yellow-throated Marten.But there are some definite differences in base composition,the using of Initiation codon and Stop codon,and the mode of repeat sequences in control region.The codon usages of Manes have bias,and the ttiird locations of codon of protein-coding genes have the higher frequency in using A and C.There may be some relativity with the content of A and C in D-loop,namely,it has relation with the mode of repeat sequences.The complete mitochondrial genome of the Sable and Yellow-throated Marten were submitted to GenBank,and the accession number are FJ429093 and FJ719367 respectively.3、The complete mitochondrial genome of 6 other species of Mustelidae from GenBank and some sequences of D-loop from the 6 species were aligned.

分析紫貂大兴安岭亚种、长白山亚种、阿尔泰亚种和北欧亚种间的基因流及进化历史得知：大兴安岭种群与新疆种群和长白山种群间的基因流水平最高(Nm=0.1260和0.1427)，新疆与长白山种群间最低Nm=0.0053紫貂种群在进化过程中可能发生过种群膨胀，经历过种群增长过程。2、对紫貂和黄喉貂的线粒体全基因组结构进行分析发现：全长分别为16 523bp和16549bp，均包含13个蛋白质编码基因、2个rRNA基因、22个tRNA基因和1个非编码序列区(D-Loop区，紫貂全序列中碱基组成为A-32.0%，C-27.6%，G-14.7%，T-25.8%，黄喉貂为A-32.5%，C-26.9%，G-14.1%，T-26.5%；基因排列顺序与日本貂和貂熊的一致，但碱基组成、起始密码子和终止密码子的使用及控制区中串联重复序列模式等均存在一定差异。

Mitochondrial 16S rRNA gene and COⅠ gene fragments were amplified and sequenced from 4 wild populations of Portunus trituberculatus. Respectively, 524bp and 658bp long partial gene fragments of the 16S rRNA gene and the COⅠ gene were obtained. A high percentage of A+T base composition in the two sequences was observed, which was similar to the results of studies on drosophila, shrimp, crab and other invertebrates.

对分别采自辽东湾、莱州湾、海州湾和舟山的三疣梭子蟹4个野生群体的线粒体16S rRNA和COⅠ基因片段进行了扩增和测序，分别得到长度为524bp和658bp的片段。2段序列的碱基组成均显示较高的A+T比例（16S rRNA基因70.8%，COⅠ基因63%），这与果蝇、虾类、蟹类等无脊椎动物的16S rRNA和COⅠ基因片段研究结果相似。

In nature, forest is resource-base, gene-base and power-base which take on the most perfect function. It is very important to maintain terrene environment and ecologic balance. Forest, as the provider of natural resource which is necessary to mankind development, take on significance to social sustainable development.

森林是自然界功能最完善的资源库、基因库、蓄水库和能源库，对维持陆地生态环境，维持生态平衡起着重要的作用，同时森林作为人类发展必不可少的重要自然资源提供者，对社会经济的可持续发展具有极其重要的意义。

Label with LC-Red640 and LC-Red750 two are aimed at above all 112 code with 158 child detect 5 ′ carry bougie, corresponding anchor decides bougie to be labelled by fluorescence in 3 ′ end, circulate quickly from inside DNA of person gene group again PCR is distinctive the enlarge that make thing adds part of ApoE gene 265bP and with distinctive bougie cross hind, cause fluorescent resonance energy to transfer, the occurrence that passes different source cross and fault match alkaline base the fusible parameter of the type will finish feral model divide with choppy gene model.

首先以LC-Red640和LC-Red750标记两条针对112和158位编码子的检测探针5′端，相应的锚定探针在3′端被荧光标记，再从人基因组DNA中快速循环PCR特异引物扩增apoE基因265bP片段并和特异探针杂交后，引发荧光共振能量转移，通过异源杂交的出现和错配碱基的类型的熔解参数来完成野生型和突变基因分型。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。