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In an effort to simplify the procedure and to reduce the cost of fluorescence SSR analysis,the conditions of the multiplex PCR and the multiplex gel electrophoresis were optimized in the genetic analysis of sunflower Helianthus annuus L.

在向日葵(Helianthus annuus L。)自交系SSR遗传分析中，为了提高SSR荧光分析效率、简化分析程序和降低研究费用，我们进行了多聚PCR和多聚凝胶电泳两项技术的优化研究。

The hemocyanin was firstly isolated and purified by ultracentrifugation and gel filteration chromatography on Sepharose 4B column.

本实验通过超高速冷冻离心和Sepharose 4B凝胶过滤柱层析法从湖北钉螺血淋巴中提取、纯化了血蓝蛋白。

The protein of 14.3kDa was then analyzed by gel filtration in ion exchange chromatography, and the MALDI-TOF/TOF-MS search on the Internet. The results indicate that the protein was hemocyanin subunit.

然后用Sephadex G75凝胶过滤层析和DEAE-52离子交换层析纯化出该过敏原，再经MALDI-TOF/TOF-MS检测，利用Blastz在线分析。

After being bathed at 100 ℃ and 80 ℃ for 30 s, 1 min, 3 min and 5 min respectively, each hemolymph was centrifugated at 10 000 r/min for 10 min to get rid of unwanted proteins, the supernatant was tested against M.lysodeikticus and displayed by polyacrylamide gel electrophoresis.

置于100、80℃水浴30s、1、3和5min后，观察不同温度处理不同时间后的去杂蛋白效果和对溶壁微球菌的抑菌效果，并对各样品进行电泳分离。

Using modern biology research technique and means,plasmid extract and agarose gel electrophoresis of molecular biology level explore test were carried out for coal with not coal department the hereditary property of Thiobacillus ferrooxidans.

利用现代生物学研究方法和手段，对煤系与非煤系氧化亚铁硫杆菌的遗传特性进行了质粒抽提和琼脂糖凝胶电泳等分子生物学水平的基础性探索试验，对于质粒在硫杆菌中普遍存在的观点提出了质疑。

After that, we detect cell DNA heteroploid and cell cycle transformation of human osteosarcoma tissue by using flow cytometry method; next, we amplification the exon2 and exon3 of 〓 gene in 36 cases human osteosarcoma tissue, and amplification products were performed the sepharose and polyacrylamide gel electrophoresis immediately.

然后采用原位杂交方法，对45例骨肉瘤标本组织和10例骨纤维结构不良组织中〓基因mRNA进行检测，以了解骨肉瘤标本组织和骨纤维结构不良组织中〓基因在蛋白质水平和mRNA水平的表达情况。

With oxalic acid and hexamethylenetetramine as the catalysts, and phenolic resin and tetraethoxysilane as the starting materials, a two-step sol-gel processing is developed for preparing Si〓N〓/SiC precursors with homogeneous microstructure composed of nanometer sized particles.

用草酸和六次甲基四胺为催化剂，酚醛树脂和正硅酸乙脂为原料，探索出了制备结构均匀而精细的Si〓N〓／SiC前驱体的溶胶—凝胶两步法。

To minimize the contamination of detrimental elements such as sulfurand chlorine, a two-step sol-gel process was successfully developed to synthesize SiC precursors with oxalic acid and hexamethylenetetramine HMTA as the catalysts, which are free of sulfur and chlorine. Tetraethoxysilane TEOS and phenolic resin were used as the silicon source and carbon source, respectively.

为了尽量减少硫、氯等有害元素的含量，本文用不含有害元素的草酸和六次甲基四胺HMTA为催化剂，正硅酸乙酯TEOS为硅源，酚醛树脂为碳源，成功探索出了制备SiC前驱体的两步法溶胶-凝胶工艺。

Using inorganic aluminous salts as precursors and hexamethylenetetramine as the catalyst, alumina nanonpowder was fabricated by sol-gel procedure and then incorporated in the preparation of body batch for thickly-glazed, thin-bodied celadon wares.

以无机铝盐为先驱体，六次甲基四胺为催化剂，采用溶胶－凝胶法制备了纳米氧化铝粉体，将纳米氧化铝粉体应用于薄胎厚釉青瓷瓷胎中，研究了粉体对青瓷瓷胎的增强作用。

By adopting DNA fingerprints analysis and polyacrylamide gel electrophoresis simultaneously,the author made an genetical study on analytical evaluation of kindred relationship and contents of serum protein and esterase of a female hinny and her offspring bred after her mating with a masculine ass.

采用DNA指纹分析和聚丙烯酰胺凝胶电泳法，对一例与公驴交配生育了后代的母|JUE||TI|后代进行了亲缘鉴定和血清蛋白、酯酶遗传的分析。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。