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In contrast to the 2-DIGE (two-dimenaional gel electrophoresis) profile of Tsga2, existence of multiple pI variants of MSAP also displayed the difference in posttranslational regulation between the murine and cyprinid homologues.

利用MSAP的抗体侦测各种脊椎动物之可能同源蛋白证实各物种MSAP同源蛋白间的确有分子量差异的情形。

Single Al-Ni-Co decagonal quasicrystal particles were prepared by the combination use of conventional casting and ball milling. The coated Al-Ni-Co quasicrystalline particles were prepared by sol-gel processing.

采用常规铸造法和球磨法制备得到单相Al-Ni-Co十次准晶颗粒，并采用溶胶－凝胶法在准晶颗粒表面成功的添加了涂层。

The optical property of CdS nanocrystals in ZrO 2 thin film has been studied. The film was fabricated by sol gel technique. Photoluminescence and photoluminescence excitation spectra of the samples with different annealing temperature were measured. Photoluminescence decay curve of CdS nanocrystals at different annealing temperatures was studied.

研究了用溶胶凝胶方法制备的CdS超微粒/ZrO2薄膜的发光特性，测量了不同退火温度处理的样品的荧光光谱、荧光激发光谱和荧光衰减曲线的变化，通过荧光衰减曲线研究了140 ps左右的CdS的带间跃迁发射和较慢的表面态发射。

Finally, silver nanoparticles resulted in the leakage of cytoplasmic component. Moreover, the analysis of agar gel electrophoresis demonstrated that silver nanoparticles could increase the decomposability of genome DNA.

另外，纳米银对大肠杆菌总DNA影响的分析表明，随着纳米银浓度的增高，大肠杆菌总DNA样品降解的程度增大。

In all the methods of catalysts preparation, sol-gel process drying by high temperature decrepitation could achieve the best catalyst.

而在浸渍法和几种溶胶—凝胶法中，使用溶胶—凝胶法并使用高温烧爆法干燥的催化剂取得了最高的产量。

The best method for extracting solube proteins from leaves was selected for polyacrylamide gel was established.The result showed that there exists two decrescendo protein bands with 76.3 X 103 and 64.1 X 103 during the livelong stage. It presents two new protein bands with 47.3 X 103 and 41.4 X 103 in adventitious roots differentiating and elongation stage.

研究结果表明，在组培叶片不定根的发生过程中，从诱导阶段到根原基形成阶段和不定根分化伸长阶段存在两个逐渐减弱的蛋白谱带：76.3×10~3和64.1×10~3，在不定根分化伸长阶段出现了两条新的蛋白差异谱带：47.3×10~3蛋白和41.4×10~3蛋白。

One group was cultured in DEME free of serum; the other in DEME with 10 μg/ml lipopolysaccharides for 6 hours. NF-κB was studied by flow cytometry technique using immunofluorescent staining, and by gel shift assay using γ-32P labeled NF-κB oligonucleotide probe.

取体外培养的第2或3代人角膜基质细胞，同步化后分为2组：无血清的DMEM液培养的不加药组；含质量浓度为10 μg/ml脂多糖的DMEM液培养6小时的加药组。

Methods Proteins and peptides in CSF were separated by two-dimensional gel electrophoresis（2DE） and ultra-performance liquid chromatographer and identified by matrix assisted laser desorption ionization time of flight mass spectrometry（MALDI-TOF-MS） and electrospray ionization to study differential proteomic expression in the cerebrospinal fluid between patients with Demy- elinative disease and with other neurological diseases.

方法以中枢神经系统脱髓鞘疾病患者的脑脊液为研究对象，分别采用固相pH梯度双向凝胶电泳分离总蛋白质，凝胶经银染显色后，用图像分析软件进行比较分析、识别差异表达的蛋白质，离线应用基质辅助激光解析电离飞行时间质谱得到相应的肽指纹图谱；同时用超高压液相色谱分离酶解肽段，在线采用电喷雾四极杆飞行时间质谱鉴定肽段氨基酸序列；搜索数据库鉴定部分蛋白质。

When the temperature for denaturalization decreased to 85℃ or below, and that for annealing increased to 72℃, no visual PCR product was observed on agarose gel electrophoretic profile.

当变性温度降低至85℃及85℃以下，退火温度升高至72℃时，琼脂糖凝胶电泳肉眼观察不到扩增产物。

DNA extraction ; Gut microflora ; Denaturant gradient gel electrophoresis

DNA提取；肠道菌群；变性剂梯度凝胶电泳

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。