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Results Agarose gel electrophoresis proved that the length of PCR product was about 7 000 bp. SDS-PAGE showed that the target protein was highly expressed. Compared with those of control, the anthranilate synthase and tryptophan synthase activities of expressed product increased by 2.7 and 3.2 folds respectively.

结果 凝胶电泳可见代PCR扩增产物大小约为7000bp，SDS-PAGE鉴定目的蛋白分别得到了表达，邻氨基苯甲酸合成酶和色氨酸合成酶的活性分别比对照提高了2.7和3.2倍。

In this research, we used a sol-gel process to prepare UV-curable antiglare coatings. First the inorganic monomer tetraethoxylsilane was formed into colloidal silica by hydrolysis and condensation reactions. Then, added the couple agent 3- propyl methacrylate and surfactant fluoroalkylsilane (13F) to modify the colloidal silica. Finally, organic monomer dipentaerythritol hexaacrylate were added to form hybrid material.

中文摘要本研究是经由溶胶凝胶制程，将无机单体四乙氧基矽烷先行水解、缩合反应成为二氧化矽，接著分别加入偶合剂三异丙基矽基丙烯酸酯、氟化矽氧烷(13F)、对二氧化矽微粒进行表面改质；之后再导入交联剂DPHA加以强化合成出奈米混成涂料。

Methods:To produce antihuman DR5 monoclonal antibody(mDRA6) by immunizing BALB/c mice using DR5 protein. The change of DR5 expression after DDPtreated on HL60 cells was detected by FACS. Morphologic changes of HL60 cells were observed under fluorencence microscope. Cytotoxic and apoptotic effects of mDRA6 and DDP on HL60 cells were measured by MTT analysis. DNA fragmentation was detected by agarose gel electrophoresis.

DR5蛋白免疫BALB/c小鼠，融合筛选抗DR5杂交瘤细胞，制备抗DR5单抗——mDRA6；流式细胞术测定顺铂对HL60细胞表面DR5表达及细胞凋亡率；荧光显微镜下观察mDRA6与顺铂协同作用下HL60细胞形态变化；MTT法测定不同浓度的顺铂与mDRA6对HL60细胞存活的影响；琼脂糖凝胶电泳检测mDRA6与顺铂联合对HL60细胞DNA片段化的影响。

These antiperspirant gel-solid stick compositions provide improved low residue performance, antiperspirant and aesthetics.

该防汗凝胶－固体棒状组合物提供了改进的低残留物性能、功效和美学。

Objective To observe the antiphlogistic effects of the Ditong rhinitis gel.

摘 要：目的观察滴通鼻炎凝胶的抗炎作用。

GELNIQUE is a quick-drying, clear and colorless, fragrance-free hydroalcoholic gel containing oxybutynin chloride, an antispasmodic agent.

美国FDA批准沃森公司的10%氯化奥昔布宁凝胶（oxybutynin chloride，Gelnique）上市，首个和唯一用于治疗以尿急、失禁和尿频为症状的膀胱过度活动症。

In this paper, the catalytical esterification of palmitic acid with 1 hexadecanol by lipase immobilized by microemulsion base organo gel that consisted of bis (2 ethylhexyl)sodium sulphsuccinate/heptane/water/gelatin in apolar organic solvent was studied.

研究了以AOT二(2 乙基已基琥珀酸酯磺酸钠/正庚烷/水/明胶组成的反相微乳凝胶固定化脂肪酶，在有机溶剂中催化棕榈酸和十六醇的酯化反应。

Based on the cloning FeSOD gene of the HZNH, the recombinant prokaryotic expression vector, PQESOa/FeSOD, was constructed and digested with restriction endonuclease BamH I and Pst I to check its construction. The PQE30a/FeSOD was then transformed into E.coli Ml5 and induced with IPTG. The high expression in vitro was obtained and analyzed on SDS-PAGE gel. The*results showed that the target proteins held a 37% portion in whole bacterial proteins and consisted of two parts, the soluble proteins and inclusion bodies. The soluble proteins in the aqueous layer, checked by means of activities of FeSOD enzymes and analyzed by means of activities of isozymogram from PAGE, demonstrated the induced expression proteins had the active nature of FeSOD enzymes.

以克隆的特异种质烟草HZNH的FeSOD基因为基础，构建了原核表达载体PQE30a／FeSOD，经限制性内切酶BamH Ⅰ、Pst Ⅰ双酶切鉴定后，再转化入大肠杆菌M15中，通过IPTG诱导，得到高效体外表达，经SDS-聚丙烯酰胺凝胶电泳检测，表达的目的蛋白占总菌体蛋白的37％，可溶性和包涵体两种形式均有存在，上清中的可溶性蛋白经FeSOD酶活测定和同工酶活性谱带分析，表明诱导表达的上清中的目的蛋白为有活性的FeSOD酶。

The experimental results show that it is necessary to treat the formation with the temporary plugging agent to seal the pay zones for selectively injecting gel into aqueous layers when the effluent water pressure is higher than that of pay zones, or the fractures in oil-bearing zones are much wider than those in the aqueous layer.

在油井水层出水压力高于油层压力或者产油层裂缝大于出水层裂缝情况下，采用笼统注入方式堵水须进行暂堵处理，以封住产油层，使堵剂主剂选择性进入水层。

The chloroform layer and the aqueous layer were separated by multiple column chromatography including macroporous resin, silica gel and polyamide column and preparative HPLC. The compounds obtained were identified by mass spectra and NMR data.

大青叶乙醇提取物，用氯仿萃取后得到氯仿层和水层，各层再分别采用硅胶柱、大孔树脂柱、聚酰胺柱、制备液相、葡聚糖凝胶柱层析及重结晶等分离手段进行分离、纯化，并应用化学性质和质谱、波谱法（1H-NMR、13C-NMR、HMBC）鉴定其化学结构。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。