查询词典 gel

Chemiluminescent immunoassay is one of the advanced immunoassay ofnon-radioisotopic immunoassay because of its high sensitivity, wide dynamic range,high accuracy, stable labeled protein, full automation and extensive application field.CLIA using acridinium ester derivatives as chemiluminescent label has advantages oflow background, high signal-to-noise ratio, no need of catalyst and simpleluminescence system. Different aspects associating the DMAE·NHS-based CLIA were studied in thisthesis, including synthesis of acridinium ester, chemiluminescentcharacteristics of DMAE·NHS, labelling antibody or streptavidin with DMAE·NHS,two-site sandwich chemiluminescent immunoassay for TSH, two-sitesandwich chemiluminescent immunoassay involved biotin-streptavidinsystem for TSH, and competitive chemiluminescent immunoassay usingbiotin-streptavidin system for TT〓. The desired acridinium ester, DMAE·NHS, was synthesized according to thereference method with some modifications. The products were identified by IR, NMR,MS and elemental analysis. In our method, KOH was used in place of NaOH tosynthesize benzyl ester of 3,5-dimethyl-4-hydroxybenzoic acid,2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-acridinium-9-carboxylate waspurified on a silica gel column with chloroform/ethylacetate(4:1, v/v) as eluent andfurther purified by triturating with hexane/acetone(2:1, v/v).

DMAE·NHS的合成是本论文的关键和难点，我们对文献方法进行改进：文献方法用氢氧化钠与3，5-二甲基-4-羟基苯甲酸反应制得3，5-二甲基-4-羟基苯甲酸钠，再用3，5-二甲基-4-羟基苯甲酸钠与苄氯作用制备3，5-二甲基-4-羟基苯甲酸苄酯，我们用氢氧化钾代替氢氧化钠，使合成取得成功；在合成2'，6'-二甲基-4'-苯基-吖啶-9-甲酸酯时，文献方法对粗产品进行两次硅胶柱层析纯化，第一次柱层析用氯仿／乙酸乙酯(4∶1，V∶V)作溶剂和淋洗剂，第二次柱层析用己烷／丙酮(2∶1，V∶V)作溶剂和淋洗剂，按照文献方法得到的不是所需要的化合物，我们只进行第一次柱层析纯化，然后用己烷／丙酮(2∶1，V∶V)进行研磨，过滤，洗涤，除去溶于己烷／丙酮(2∶1，V∶V)的部分，得到所需要的产品。

The research results indicated that the first cross-linking between the HPAM and Cr3+ in ground condition was propitious to reduce leaking of blocking agent in oil layer, and the second crosslink action between acylamino of HPAM and organic phenolic aldehyde can form the high intensity gel and it can endure high temperature.

研究结果表明，HPAM羧基与Cr~(3+)离子在地面条件下的一次交联作用有利于减少堵剂在油层的漏失量，HPAM酰胺基与有机酚醛在地层条件下的二次交联作用，能够形成耐温高强度凝胶。

Objective: To explore the feasibility of the combination of psuedo-ligand affinitive chromatography and gel chromatography in purifying RIP from bitter gourd and the cytotoxicity of the RIP.

结果： 运用硫酸铵分级沉淀、Blue Sepharose CL-6B假配基亲和层析、Sephadex-G75凝胶层析等方法提取、纯化得到了高纯度的苦瓜RIP，并且能够获得较高的得率；一定作用剂量的苦瓜RIP不仅能够抑制HL-60细胞的增殖活性，还可以诱导其发生凋亡。

Using ammonium sulfate precipitation, Blue Sepharose CL-6B psuedo-ligand affinitive chromatography and Sephadex G-75 gel chromatography, we obtained the typeⅠRIP with high purity, the feasibility of this method has been approved by SDS-PAGE results.

结果显示：1。运用硫酸铵分级沉淀、Blue Sepharose CL-6B假配基亲和层析、Sephadex-G75凝胶层析等方法提取、纯化得到了高纯度的苦瓜RIP，并且本方法提取效率较高，所获得的RIP在SDS-PAGE上呈现为单一条带。

The carboxyl groups of HPAM replace the H〓O ligands of AlCA, which results in the formation of HPAM-AlCA gel.

首次用实验证明了：HPAM大分子中与AlCA反应的基团为羧基，酰胺基不参与交联反应。

Dynamic viscoelasticity of curvielan,a novel gel-forming exopolysaccharide produced by soil bacterium Alcaligenes sp.Q9415 was studied.

对产碱杆菌Q9415胞外多糖的动态粘弹性进行了研究。

39 Qinghai Alectoris graeca were used to study isozyme pattern of serum amylase and activity with vertical polypropylene gel electrophoresis and spectrophotometry, respectively.

方法］采用聚丙烯酰胺凝胶垂直平板电泳法对39只青海石鸡血清淀粉酶同工酶酶谱进行研究，并利用比色法时其淀粉酶活性进行测定。

The characters of the composition, which is double component sealant made from 1-3% alginic acid sodium and 20% CaCl2 solution, is best in the biological experiment. Before new medical glue appeared in clinical, this gel is hopeful to be applied in clinic and provide theory basis for clinical anastomosis.

质量分数为1%~3%的海藻酸钠水溶液与质量分数为20%的CaCl2组成的双组分密封胶，生物实验中各项性能良好，在新的医用胶面世以前，可以作为血管粘接吻合密封剂，有望在临床中实验推广，为临床血管粘接吻合应用提供理论依据。

Using normal alkalic shower gel will destroy the weak acidic environment and weaken the protective action.

但使用一般碱性沐浴露，会破坏私处的弱酸环境，减弱保护作用。

The porous TiO2 anatase nanometer thin films were prepared from alkoxide solutions containing polyethylene glycol by sol-gel method.

锐钛矿型多孔TiO2纳米薄膜可以从含聚乙二醇的钛酸盐溶胶前驱体中通过溶胶-凝胶法制备。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。