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objective effects of plant tannins,chebulinic acid and tellimagrandin i on chemically induced hemoglobin synthesis in k562 cells were investigated.methods the hemoglobin synthesis situation was assayed with benzidine staining.erythroid antigens glycophorin a expression on the surface of k562 cells was labeled by direct immunofluorescence using fluorescein isothiocyanate-conjugated anti-gpa antibodies.then flow cytometric analysis was performed to detect gpa expression levels on surface of the cells.results both chebulinic acid and tellimagrandin i could inhibit the hemoglobin synthesis of butyric acid and hemin-treated k562 cells in a concentration-dependant manner.however,the ba-induced k562 cells were more sensitive to two tannins than heroin-induced cells.conclusion chebulinic acid and tellimagrandin i have inhibitory effect on the erythroid differentiation.

目的 研究可水解单宁诃子酸和特里马素i对氯化高铁血红素和丁酸钠诱导k562细胞红系分化的影响。方法四甲基偶氮噻唑蓝法分析诃子酸和特里马素i对k562细胞生长的影响,联苯胺染色法检测血红蛋白合成情况,应用免疫荧光和流式细胞术检测血型糖蛋白a在细胞表面的表达。结果诃子酸和特里马素i在0.04~0.09?mmol/l浓度下均可显著抑制k562细胞的生长增殖。2种单宁化合物(0.002~0.01?mmol/l)可显著抑制丁酸钠诱导的k562细胞血红蛋白合成,2种单宁化合物(0.01~0.05?mmol/l)还可显著抑制氯化高铁血红素诱导的血红蛋白合成,特里马素i(0.01?mmol/l)还抑制丁酸钠诱导的gpa在细胞表面表达。结论诃子酸和特里马素i对红系分化有抑制作用。

And Fig. 58 126,128. The lining cells include nonciliated mucous columnar cells, goblet cells, absorptive-type cells, cuboidal cells, and Paneth cells.

衬覆细胞包括:无纤毛的粘液柱状细胞、杯状细胞、吸收细胞、立方细胞和罕见的潘氏细胞。

In the rice leaves infected by RDV Yunnan isolate, the distribution of cells full of virus particles formed a belt which was a center of infection and multiplication of virus, around the cells fall of virus particles were some cells with the different extent of lesion as follows: disappearance of membrane system of chloroplasts, there were virus particles in some chloroplasts, and some sine or assembled virions in the mitochodria or cytoplasma but not in nuclei ,and intact subcellular structure in the cell far away from the infected center; In the rice leaves cell infected by Zhejiang isolate, some cells were full of virions, but the distribution of cells didn't form the belt, other cytopathological characters were similar to the cells infected by Yunnan isolate.

RDV云南分离株浸染的水稻叶片中,充满病毒粒体的细胞呈条带状分布,呈现出明显的侵染和增殖中心,在充满病毒粒体的细胞周围,相邻细胞有不同程度的病变,叶绿体膜系统消失,部分叶绿体中含有病毒颖粒,线粒体残体及细胞质中有分散或聚集的病毒粒体,细胞核中未见病毒粒体,离侵染中心较远的细胞中亚细胞结构较完整;RDV浙江分离株侵染的水稻叶片中,部分细胞充满病毒粒体,无明显的条带状分布,其他细胞病理特征与RDV云南分离株侵染的水稻叶片相似。

Results: Mast cells in uterus were mainly distributed in the myometrium, only a few in the endometrium. After ovum implantation ,the mast cells were comparatively less at the place of implantation. The number of mast cells of early and late pregnancy(1~5 dyas, 15~19 dyas) was apparently higher than that during middle pregnancy(P<0. 05). Stained by alcian blue-safranin, both red and reddish blue mast cells decreased gradually in number during pregnancy, but the proportion of blue mast cells was increased. Critical electrolyte concentration of mast cells decreased as time went on.

结果:(1)肥大细胞主要分布于子宫肌层,子宫内膜较少,且胚泡植人部位的肥大细胞明显少于非植入部位;(2)妊娠早期(孕1~5d)和妊娠后期(孕15~19d)肥大细胞数显著多于妊娠中期(P<0.05);(3)Alcian蓝藏红组化染色后,可见妊娠过程中红色和红蓝混合色肥大细胞逐渐减少;蓝色肥大细胞所占比例逐渐增大,孕9~19d肥大细胞几乎全部为蓝色;(4)肥大细胞临界电解质浓度值随妊娠时间的推移而变小。

The cells of isolate (CW2) stain Gram positively, the morphology of the cells of isolate was changed from rod to round in the process of growth, the cells in the initial phase of logarithmic growth were long rod, majority of cells singly existed, minority of cells were geminated or formed a long discontinuous chain, until to invariable phage of growth, the cells changed to globe.

分离出的菌株CW2为革兰氏阳性,在生长过程中细菌细胞的形态有球杆的变化,在对数生长期的初期细胞是直杆或者弯杆状,大多数单生,少数成对或者成不连续的链状,到恒定期变成类球形细胞。

And the different concentrations of natural monoclonal anti-keratin antibody IgM 3B4 were incubated with the mixed suspenions of Candida albicans yeast phase with malpighian cells, human buccal epithelial cells, endothelial cells of fetal umbilical vein, respectively, to observe the action of natural monoclonal anti-keratin antibody IgM 3B4 to the adhesion of Candida albicans to malpighian cells, human buccal epithelial cells and endothelial cells of fetal umbilical vein.

结果:与对照组比较,不同浓度的单克隆天然抗角蛋白抗体IgM3B4均能抑制白念珠菌芽管生成,并且抗体浓度越高,抑制白念珠菌芽管形成的作用越明显;同时单克隆天然抗角蛋白抗体IgM3B4能够显著减少白念珠菌与角质形成细胞、上皮细胞、内皮细胞的粘附,其抑制作用与单克隆天然抗角蛋白抗体IgM3B4的浓度呈正相关。

Keratin antibody IgM 3B4 to the adhesion of Candida albicans to malpighian cells, human buccal epithelial cells and endothelial cells of fetal umbilical vein. RESULTS: It was found that the different concentrations of natural monoclonal anti??keratin antibody IgM 3B4 could inhibit Candida albicans germ tube formation and adhesion of Candida albicans to malpighian cells, human buccal epithelial cells and endothelial cells of fetal umbilical vein.Its inhibitory action was positively related to the level of natural monoclonal anti??

结果:与对照组比较,不同浓度的单克隆天然抗角蛋白抗体IgM 3B4均能抑制白念珠菌芽管生成,并且抗体浓度越高,抑制白念珠菌芽管形成的作用越明显;同时单克隆天然抗角蛋白抗体IgM 3B4能够显著减少白念珠菌与角质形成细胞、上皮细胞、内皮细胞的粘附,其抑制作用与单克隆天然抗角蛋白抗体IgM 3B4的浓度呈正相关。

METHODS: The different concentrations of natural monoclonal antikeratin antibody IgM 3B4 were incubated with Candida albicans yeast phase suspension on condition that profited germ tube formation of Candida albicans to observe the action of natural monoclonal antikeratin antibody IgM 3B4 to Candida albicans germ tube formation.And the different concentrations of natural monoclonal antikeratin antibody IgM 3B4 were incubated with the mixed suspenions of Candida albicans yeast phase with malpighian cells, human buccal epithelial cells, endothelial cells of fetal umbilical vein, respectively, to observe the action of natural monoclonal antikeratin antibody IgM 3B4 to the adhesion of Candida albicans to malpighian cells, human buccal epithelial cells and endothelial cells of fetal umbilical vein.

将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4分别与白念珠菌酵母相悬液在有利于芽管形成的条件下共同孵育,倒置显微镜下计数白念珠菌总数以及白念珠菌芽管数;将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4与白念珠菌酵母相和人角质形成细胞混悬液、白念珠菌酵母相和人口腔黏膜上皮细胞混悬液、白念珠菌酵母相和胎儿脐静脉内皮细胞混悬液共同孵育,分别计数人角质形成细胞、人口腔黏膜上皮细胞、胎儿脐静脉内皮细胞上粘附的白念珠菌数。

Results The expression of the Mahoganoid protein and its mRNA was showed by brown staining and observed in the Leydig cells, spermatogonia, primary spermatocytes, spermatids, Sertoli cells, and peritubular myoid cells of testicular tissues and principal cells, basic cells and epithelial cells of epididymal tissues.

结果1。免疫组化法检测Mahoganoid蛋白的实验结果显示的棕色阳性信号可见于各天龄段大鼠睾丸的生精细胞、支持细胞、间质细胞和管周肌样细胞以及附睾输出管的高柱状、低柱状上皮细胞和附睾管的上皮细胞、主细胞、基细胞。其中Mahoganoid蛋白主要表达于胞膜和胞浆。

We found that 1 mutant EG4 cells showed typical characteristics of pluripotent stem cells which had no obvious difference with wild cells; 2 When induced by 10〓 M retinoic acid , mutant EG4 cells differentiated into adipocytes with high frequency compared with mutant cells, suggested that EGFR plays a role in adipocyte differentiation; 3 when injected into nude mice, mutant teratocarcinomas contained a large amount of connective tissues as well as skeletal muscle, while wild EG4 cells produced frequently cartilage, keratinocyte and neuroepithelium.

我们建立了稳定表达胞内区功能缺失的外源EGFR cDNA片段的EG4细胞,分析其生长分化特性,发现 1)突变型细胞可在未分化状态下维持长时间的增殖,表明EGFR对EG多能干细胞表型无明显影响;2)〓 M的维甲酸A(retinoid acid A,RA)诱导后,大部分对照组细胞分化为脂肪细胞,而突变型细胞分化为脂肪细胞的比例明显较少,表明EGFR在脂肪细胞的发育分化中具有一定的调节作用;3)畸胎瘤切片分析显示,突变型瘤组织分布有大量的未分化细胞和结缔组织,分化细胞以肌肉细胞为主;对照组瘤组织含丰富的角质上皮、软骨、神经管等依赖EGFR的分化组织。

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