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The different fishes which are employed in the present studies are wild-type salmon, cultured salmon of freshwater and seawater, sea perch and fat greenling. The complete CT gene sequences of salmons are obtained by PCR amplification. The partial CT sequences of sea perch and fat greenling are obtained by in vitro cloning PCR method. Alignment of obtained CT sequences with other fish CT shows that CT appears to be well conserved among the same family. And the relative in taxonomy is far away, the similarity of different fish CTs is low. On the contrary, the closer the relative in taxonomy is, the higher the similarity of different fish CTs is. The sCT is expressed in pGEX-4T-X by recombinant form . We also succeed in the research of sCT expression alone by expression PCR. In addition, sCT antiserum is obtained using GST-sCT as antigen, and the high titer is tested by double immunodiffusion. In the rat bioassay, administration of 50 μg recombinant protein evoked significant hypocalcemia at 1 h after the data are analyzed by t-test.

本文用PCR方法克隆了野生鲑鱼、养殖鲑鱼的降钙素基因，并应用体外克隆PCR的方法首次克隆出鲈鱼、六线鱼降钙素的部分基因序列，通过对克隆的降钙素序列的比较研究，结果显示同一科的鱼降钙素序列保守性较高，同时，根据降钙素的部分氨基酸序列进行了降钙素相似性的比较研究，结果显示在分类学上，分类地位较远的鱼，其降钙素相似性较低，分类地位越接近的鱼，其降钙素相似性越高；我们利用谷胱甘肽S-转移酶(Glutathions S-transferase，GST)融合表达载体pGEX-4T-X对克隆的鲑鱼降钙素基因进行了融合表达研究，应用表达PCR的方法对降钙素基因的独立表达进行了初步的研究探索，并将纯化的融合蛋白作为抗原，获得了高效价的兔抗鲑鱼降钙素免疫血清；生物活性研究表明，大肠杆菌表达的融合蛋白具有显著的降血钙作用。

Conversely, if ''S'' is a subgroup of ''G'', then it obeys the axioms of a group.

因此，满足了闭包、单位元和逆元公理，而结合律是继承来的，所以''S''是子群。

A critique of Handlin's interpretation of why legal slavery did not appear until the 1660's suggests that assumptions about the relation between slavery and racial prejudice should be reexamined , and that explanations for the different treatment of Black slaves in North and South America should be expanded .

一个对于H的关于为何法律上的奴隶制没有在17世纪60年代以前出现的原因所作解释的批评显示，关于奴隶制和种族偏见之间的关系的假说应当重新检查，而且显示出，对于在北美和南美之间的对黑怒的不同处理的解释应当被扩展。0 Y/ m# q2 N3 g# X

When the cooling rates are 1.0 × 10^14 K/s and 1.0 × 10^13 K/s, the amorphous structures are formed mainly with the 1551 and 1541 bondtypes or polyhedron basic cluster （ 13 1 10 2） and icosahedron cluster （ 12 0 12 0） in the system.

采用双体分布函数g曲线、Honeycutt-Andersen键型指数法和原子团类型指数法对凝固过程中微观结构的变化进行了分析。

2 Presser Foot, Types A, B, C, D, DO, E, O, OO, OOO, and OOO-S, with an orifice (to allow for the protrusion of the indentor) having a diameter as specified in Fig.

A型，B型，C型，D型，DO型，E型，O型，OO型，OOO型，OOO-S型的压足和配合孔的尺寸见FIG.1（a，b，c，d，e，f和g）的规定，压足中心与测量试样任意边缘最小距离为6.0mm（0.24in），当压足设计成圆形平面时，它的的面积不应小于500mm2 19.7in2

HH Farmer, Towards Belief in God; R Hazelton, On Proving God; J Hick, The Existence of God; D Hicks, The Philosophical Basis of Theism; AJ Hoover, The Case for Christian Theism; S Jaki, The Road of Science and the Ways to God; CEM Joad, God and Evil; J Maritain, Approaches to God; EL Mascall, The Openness of Being; G Mavrodes, The Rationality of Belief in God; A Plantinga, ed., The Ontological Argument; RC Sproul, If There Is a God, Why Are There Atheists?

个HH农民，对信仰的上帝与r黑兹尔顿，就证明上帝； j hick ，上帝存在； d希克斯，哲学基础的有神论；的AJ胡佛情况基督教有神论； s jaki ，道路的科学和如何向上帝；杰姆joad ，上帝与邪恶； j旦，途径上帝下午马斯科尔，公开性被100 mavrodes ，理性的信仰上帝；普兰丁格，版，本体论的论点；钢筋sproul ，如果有一个上帝，为什麼有无神论者？

S Benko, Protestants, Catholics and Mary; L Bouyer, The Seat of Wisdom; EA Carroll,"A Survey of Recent Mariology," MarS 18, and "Theology on the Virgin Mary: 1966 - 1975," TS 37; JB Carol, Fundamentals of Mariology and Mariology; H Graef, Mary: A History of Doctrine and Devotion; R Laurentin, The Question of Mary; G Miegge, The Virgin Mary; TA O'Meara, Mary in Protestant and Catholic Theology; O Semmelroth, Mary, the Archetype of the Church; E Schillebeeckx, Mary, Mother of the Redemption.

s benko ，新教徒，天主教徒和玛丽；升bouyer ，所在地的智慧；环境卡罗尔，"统计调查结果显示，最近mariology ，"火星18 "神学对圣母玛利亚： 1966年-1 975年，" 6月2 4日t s3 7； j b卡罗尔基本面mariology和mariology ； h graef ，玛莉：历史，理论和奉献精神； r洛朗坦，问题的玛丽；克miegge ，圣母玛利亚；电讯管理局局长o'meara ，玛丽在新教和天主教神学； o泽梅尔罗特，玛丽，原型教会电子商务施雷贝克，玛丽的母亲赎回。

Pisier\'s results,under tree martingale valued space X is isomorphic toan a-uniformly convex space (2≤a＜∞),some quasi-normal or normal inequalitiesof a-variation maximal operator and a-conditional variation maximal operator of X-valued predictable tree martingales are identified.

同时，用G.Pisier's结果在树鞅取值空间X同构于a一致凸Banach空间条件下，证明了几个X-值树鞅α-方极大算子和a-条件方极大算子的拟范数不等式与范数不等式。

W/ S! g## O despicable:value＝mulish:flexibility

S； _ g9 L5 r 可鄙的没有价值＝执拗的没有灵活性

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。