查询词典 fusion

The recombinant pET-Lip vector was transformed into E.coliBL21 and induced to express by 1mmol/L IPTG at 37℃. An expected 80kDa fusion proteinwas expressed. SDS-PAGE analysis showed the fusion protein located in the supernatant ofbacteria lysate by sonication. The fusion protein was purified by HisTrap~ HP Kit and coulddegrade tributyrin.

该菌株经IPTG诱导可表达分子量约80 kDa的融合蛋白，SDS-PAGE分析表明融合蛋白位于菌体超声裂解后的上清中，用蛋白纯化试剂盒HisTrap~ HP纯化后得到单一的目的蛋白，约80 kDa，且纯化的融合蛋白具有脂酶活性，可以分解三丁酸甘油酯。

Results. No changes were observed at the segments located below the fusion. All the unfused segments above the fusion showed the same significant loss of disc height. Loss of disc height did not depend on fusion parameters, correlated weakly with age and length of follow-up, and correlated highly across adjacent unfused segments.

结果：在融合节段下方的未融合节段未发现任何改变，所有融合节段上方的未融合腰椎的椎间盘高度明显丢失，椎间盘高度的丢失与融合所使用的器械无关，与患者的年齡的随访的时间有一定联系，与距离融合节段的远近关系密切。

Results. No changes were obsered at the segments located below the fusion. All the unfused segments aboe the fusion showed the same significant loss of disc height. Loss of disc height did not depend on fusion parameters, correlated weakly with age and length of follow-up, and correlated highly across adjacent unfused segments.

结果：在融合节段下方的未融合节段未发现任何改变，所有融合节段上方的未融合腰椎的椎间盘高度明显丢失，椎间盘高度的丢失与融合所使用的器械无关，与患者的年齡的随访的时间有一定联系，与距离融合节段的远近关系密切。

The detection capability of single buoy is limited. To improve detection performance, we studied the detection-level fusion of several buoys based on the position and information characteristics of several airdropped buoys. On the basis of the analysis to distributed detection fusion theory, we studied the structure, rule and algorithm of the buoy signal detection-level fusion based on distributed detection.

针对单枚浮标对目标检测能力有限的问题，为了提高对目标的检测性能，根据多枚空投浮标的位置和信息特点，对多枚浮标的检测级融合进行了研究；在对分布式检测融合理论进行研究的基础上，研究了基于分布式检测的浮标信号检测级融合体系结构、融合规则、融合算法等。

According to the deficiency of Image Fusion applications in entertainment equipments, such as no special research that combines Virtual Reality, Self-control Photographing, Computer Vision and Image Fusion technologies together, we propose an Image Fusion algorithm base on Character of Apperceiving Contrast, which take system elements into account, e.g. integrity image quality balance and costs.

鉴于目前对图像融合技术在娱乐领域的应用缺乏研究，尤其缺乏将虚拟现实技术、自助照相技术、机器视觉技术和图像融合技术综合运用的分析研究，因此我们从分析人类视觉特征入手，运用系统的观念综合平衡图像质量、系统成本等技术指标，在对虚拟实时自助照相系统具体技术指标深入分析的基础上，提出了基于对比度感知特征（Character of Apperceiving Contrast）的图像融合算法。

Firstly, every wavelet base is employed to fuse the multi-source image based on local energy rule, and then a set of the primary fusion images are obtained. Next, these primary fusion images are fused to obtain final fusion image within spatial domain based on local varianc's weighted average rule.

该方法首先用每个小波基对源图像进行基于局部能量的融合，得到多幅初级融合图像，然后对初级融合图像在空间域内再进行基于局部方差加权平均融合，得到最终融合图像。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

The array chip takes a flexible transparent polyimide thin membrane as the base, the lower surface can form a cross comb-shaped array multimicroelectrode, the electrode group is composed of two comb-shaped microelectrode array electrodes which are mutually crossed but not contacted with the electrical structures not being connected, and the microchannel between the both internal electrodes of the electrode group serves as the service passage; the array chip is inversely buckled on the fusion pool, and the cross comb-shaped array multimicroelectrode on the array chip correspondes to the cell electric fusion pool and falls in the cell electric fusion pool.

阵列芯片以柔性透明聚酰亚胺薄膜为基底，下表面通过蚀刻形成交叉梳状阵列化微电极组，电极组由两个相互交叉、互不接触、电气结构上互不连接的梳状微电极阵列电极构成，电极组内部微电极之间的微通道为工作通道；阵列芯片倒扣于融合池上，其上的交叉梳状阵列化微电极组与细胞电融合池相对应，落于细胞电融合池中。

The minor core protein s C-encoding gene of Muscovy duck reovirus was cloned into theprokaryotic expression vector pET32a. The recombinant plasmid pET32a-s C was amplified andextracted after being transformed into E.coli DH5a competent cells. Restriction analysis withEcoRⅠand SacⅠand sequences analysis indicated that the recombinant plasmid was inserted withcorrect open reading frame. The fusion protein about 50 ku was produced after induction with 0.15mmol/L IPTG of E.coli competent cells transformed with pET32a-sC. The SDS-PAGE andWestern-bloting test indicated that the fusion protein reacted with the convalescents sera of duckinfected with Muscovy duck reovirus. The indirect ELISA method was developed by using thepurified fusion s C protein as coating antigen. The optimal concentration of s C was 5μg/ml, thedilution of serum sample was 1:40; The results showed that preparation of an ELISA by using sCas coating antigen in detecting 50 field duck sera in comparison with the AGIP were more sensitiveand specific than agar gel immuno-diffusion AGIP test. The results suggest that presence ofantibody against viral protein sC in duck may be a good indicator by the sC-ELISA for detectionof duck infection with reovirus.

同时，本研究将编码外壳蛋白σC的基因克隆于原核表达载体pET32a上，经过EcoRⅠ和SacⅠ双酶切鉴定和序列分析后，得到阳性重组质粒pET32a-σC；将阳性重组质粒pET32a-σC转化到大肠杆菌BL-21感受态细胞中进行诱导表达，经SDS-PAGE和Western-blbtting检测分析，融合表达的蛋白能够与番鸭呼肠孤病毒感染的康复鸭血清发生特异性反应；将融合表达的蛋白纯化后作为包被抗原，建立了检测鸭血清中呼肠孤病毒抗体的间接酶联免疫吸附试验检测方法，此方法中抗原的最佳包被浓度为5μg／ml、标准阳性血清的最适稀释倍数为1：40倍，用此方法对50份鸭血清样品进行检测，并与琼脂糖凝胶扩散试验检测抗体的法相比较，证明此ELISA方法具有良好的特异性和敏感性，本研究为今后鸭呼肠孤病毒诊断试剂盒的研制奠定了基础。

Methods A total of 45 patients underwent cervical spine fusion surgery from Feb 2004 to Aug 2007 were reviewed retrospectively. Patients in group A (22 patients) were underwent discetomy and cage fusion, and ingroup B (23 patients) were treated with discetomy and autogenous tri-cortical iliac crestgraft fusion.

对2004年2至2007年8月45例颈椎椎体融合病例进行回顾分析，分成PEEK组和对照组，A组22例共融合32个节段，根据模具选择合适的Cage，将自体髂骨松质骨填充于Cage内并根据颈椎的稳定情况决定是否加用前路钢板。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。