查询词典 fusion

We also inserted an Enterokinase recognition site between the hSOD gene and the small peptide gene, then we can cleave away the small peptide from fusion protein easily.

在构建时还在小分子多肽与人SOD的连接区插入肠激酶识别位点，这样可以使小分子多肽能从融合蛋白中切割下来。

After the fusion protein was cleaved by enterokinase, Trx and des(1-3)MGF was isolated by reverse-phase HPLC.

再对融合蛋白EK酶切， rpHPLC分离获得纯度达98%的des(1-3)MGF， SDS-PAGE电泳及质谱分析蛋白分子量与理论值相符。

The fused insecticidal protein may delay the process of insects?developing resistance. After investigation of the secondary structure and action mode of Bt insecticidal protein and Cowpea trypsin proteinase inhibitor, as well as the action mechanism of insect enterokinase, we devised the fusion gene on the base of maintaining their functions respectively. The method follows: CpTI gene is linked to 3?-end of GFM crylA gene by a nucleotide sequence encoding cleavage site of insect enterokinase.

根据Bt杀虫蛋白Cry1A和豇豆胰蛋白酶抑制剂的高级结构、杀虫机理及昆虫体内肠激酶的作用机理，本着尽量不影响二者功能的原则，设计出了构建Cry1A和CpTI融合杀虫基因CryCI的方案，即将CpTI基因的编码序列连接到GFM Cry1A基因编码区3'端，并在其间用一段编码肠激酶切割位点的序列相连。

III For constructing the expression vector of a fusion protein and obtain a target protein with full identity on aa sequence of a natural 13- 1,3-1 ,4-glucanase, with the recombined plasmid DNA harbouring the target gene as template, the primers designed with restriction sites for both terminals and enterokinase recognition site, followed by PCR amplification, was induced to the target gene.

为构建融合蛋白表达载体和获得与天然蛋白质序列完全一致的目的蛋白，以含有目的基因的重组质粒DNA为模板，设计引物时加入两端酶切位点及肠激酶裂解位点，通过PCR扩增引入目的基因中，测序结果表明接头和读框正确。

The objective of the study was to obtain the gene of bovine enterokinase light chain,which would be used in the cleavage and purification of fusion proteins.The fragment of bovine enterokinase light chain cDNA was obtained by RT-PCR from a sold bovine′s duodenal mucosa, then cloned into the pUCmT cloning vector and sequenced.Compared with the sequence deposited in GenBank,the cloned gene sequence is correct.

为克隆表达牛肠激酶轻链编码基因，以期应用于融合蛋白的切割与纯化，从市售北方黄牛十二指肠组织中提取总RNA，以RT-PCR方法扩增其cDNA片段，将此片段克隆于pUCmT载体中，经过特异性限制性内切酶酶切分析片段正确后，进行全序列分析。

They found no difference in the incidence of degeneratie changes between the operated and the control group at leels aboe and below the fusion with the exception of anterior entophyte formation in the ACDF group.

他们发现除了在ACDE组前路的内置物不同之外，手术组与对照组在其上下节段的退行性改变发生率没有不同。

Methods:CBFβ-MYH11 fusion transcripts and inv (16) were analyzed by reverse-transcriptase polymerase chain reaction and fluorescence in situ hybridization,respectively,in acute myelomonocytic leukemia(M4) with or without eosinophilia.

用逆转录-聚合酶链反应和荧光原位杂交技术分别检测急性粒-单核细胞白血病(M4)患者的CBFβ-MYH11融合基因转录本和inv(16)。

About the age of 16 of the epiphysis and the tibial epiphyseal fusion, 18-year-old tibial tuberosity and tibial ossification as a whole.

约在16岁时该骨骺与胫骨上端骨骺融合，18岁的胫骨结节与胫骨上端骨化为一体。

After routine antituberculosis chemotherapy, the patients were treated by dead bone erasion and tuberculose focus removal of abscess, dead bone, granulation tissue and cheese-like substance, intervertebral body fusion with bone graft and Ventrofix system.

经术前正规抗结核药物化疗后行病椎死骨刮除，周边脓肿、死骨、肉芽组织及干酪样物质等结核病灶清除，椎间大块自体骨植骨或钛网植入自体骨粒植骨，一期行前路内固定术，术后继续抗结核治疗1～1.5年。

Recombinant Human Osteogenic Protein (rhOP-1) provides a strong arm to solve this difficult problem. The rhOP-1 is produced by DNA recombinant technique, with the characteristics of high purity, high output, low cost and high osteoinductive activity. How does OP-1 work on the erective spinal fusion?It has not been documented yet.

重组人OP-1（rhOP-1）的出现为这个问题的解决提供了一个强有力的武器。rhOP-1是通过DNA重组技术生产，具有纯度高、产量高、成本低等优点，具有高效的诱导成骨活性。rhOP-1在直立脊柱融合术中的作用如何，目前尚未见文献报道。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。