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In the present study, we collected cumulus cells oocyte complex from ovaries of two different strain mice. The cumulusenclosed oocytes were cultured for 6 h in MEM supplemented with growth factor and FSH. The meiotic maturation of these oocytes has progressed to pro-metaphse Ⅰ stage and the condensed chromosomes are visible under DIC microscope, metaphase Ⅰ spindle even can be detected under Polscope. The metaphase Ⅰ spindles of oocytes were exchanged under such microscopes. After electric stimuli, 91. 6% and 91. 6% karyoplasts-cytoplasm pairs were fused respectively. The resulting oocytes were cultured further in MEM and over 80% of oocytes released the first polar body. 79% and 77% of oocytes formed two pronuclei after in vitro fertilization and the embryos were cultured in KSOM supplemented with amino acids. Over 60% of embryos developed to blastocyst stage.

在本研究中我们在取得两种不同品系小鼠的卵丘卵母细胞复合体后，先将卵丘卵母细胞复合体置于含有多种生长因子和激素的MEM培养液中培养6小时，此时卵母细胞已进入第一次减数分裂的前中期，并且在DIC倒置显微镜下可以看到浓缩的染色体，用Polscope可以发现明显的纺锤体，借助这种显微镜通过显微操作将两种不同品系小鼠来源的卵母细胞的MI纺锤体进行互换，经过三次直流电脉冲作用后，分别有91.6％的胞质—MI核质体对融合，经过进一步的培养后，超过80％的重组卵母细胞排出第一极体，体外受精后分别有79％和77％的重组卵形成双原核，受精后的胚胎在KSOM胚胎培养液中体外培养4天后，超过60％的胚胎发育至囊胚。

This study was conducted to clone the sheepβ-lactoglobulin gene and the human myelomonocytic surface differentiation antigen CD14 gene (hCD14) by PCR, and this two gene were fused to construct oBLG-hCD14 fuse gene, this fuse gene were microinjected into pronuclei of mouse eggs to produce transgenic mouse.

本研究拟利用PCR和分子克隆技术，首先获得绵羊β-乳球蛋白基因调控序列片段和人CD14基因（hCD14）全程编码序列，并构建oBLG-hCD14融合基因，最后利用微量注射法将融合基因导入小鼠受精卵以产生转基因小鼠。

The possibility of using granular nitrided fused iron catalyst for Fischer-Tropsch synthesis at high space velocities is investigated.

为进行高速固定床以水煤气为原料的合成，研究了颗粒熔铁催化剂。

Methods Ten laser welded CW-PA ceramo-alloy rods were fused with porcelain at fusion zone. The porcelain-metal bond strength was measured with pull-through test. SEM examination and EDAX analysis were performed.

方法将10个激光焊接的CW-PA烤瓷合金试棒在焊缝熔区烤上瓷盘，做棒盘实验，测量金瓷结合强度，并做扫描电镜观察和X衍射能谱分析。

Butterfly-like, as the irregular corolla of a pea, with a banner petal, two wing petals, and two fused keel petals.

蝶形的 Papilionaceous 像蝴蝶，如豌豆的不整齐花冠，具有一枚旗瓣、两枚翼瓣和两枚龙骨瓣。

The fleshy part of the fruit is derived from the greatly enlarged urn-shaped receptacle, which encloses the entire fruit, and which is fused to the tough carpel walls. Thus the apple core is the true fruit.

花托与坚硬的心皮壁融合，因此在苹果果实中，其果核部分才是真正的果实部分。

Predictors forthe need for allogeneic red blood cell transfusion were ASAclassification, surgical duration and number of levels fused.

输注异体红细胞需考虑病人的 ASA 分级、手术持续时间及脊柱融合数目。

Soft'.'' thick-bodied North Atlantic fish with pelvic fins fused into a sucker; edible ''.'roe'.'' used for caviar.

软'。''又粗身体笨重的鱼，长有用作吸盘的腹鳍；鱼子可制成鱼子酱食用。

DNA vaccines with sin2 gle2chain Fv fused to fragment C of tetanus toxin induce protectiveimmunity against lymphoma and myeloma.

蛋白疫苗实验表明，在BCL1 小鼠模型中蛋白疫苗诱导出的抗独特型抗体主要作用在免疫球蛋白重链可变区 [13 ] 。

Stud/screw mounting provides the best transmissibility at high frequencies since the accelerometer is virtually fused to the mounting surface.

螺柱/螺丝安装提供了最佳的高频条件可传输率。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。