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Objective Establishment of a hybridoma cell line secreting a monoclonal antibody to facilitate iˉdentification of human tissue kallikrein（KLK1gene,hK1protein）.Methods Mice were immunized with E.coli-exˉpressed GST-hK1fusion protein and their spleen cells were fused with SP2/0myeloblastoma cells.

目的 将人组织激肽释放酶（基因命名为KLK1，酶命名为hK1）cDNA克隆入大肠杆菌表达质粒，以重组菌表达的GST-hK1融合蛋白免疫小鼠，获得了分泌hK1特异单克隆抗体的杂交瘤细胞株。

Objective Establishment of a hybridoma cell line secreting a monoclonal antibody to facilitate iˉdentification of human tissue kallikrein（KLK1gene,hK1protein）.Methods Mice were immunized with E.coli-exˉpressed GST-hK1fusion protein and their spleen cells were fused with SP2/0myeloblastoma cells.Specificity of the monoclonal antibody was shown by Western blotting and by immunofluorescence.Results The monoclonal antibody reacted specifically to E.coli-expressed hK1and with the KLK1cDNA-transfected COS-1cells.

目的 将人组织激肽释放酶（基因命名 KLK1，酶命名为hK1）cDNA克隆入肠杆菌表达质粒，以重组菌表达的GST-hK1融合蛋白免疫小鼠，获得了分泌hK1特异单克隆抗体的杂交瘤细胞株方法将KLK1cDNA克隆入真核表达质粒，用获得的重组质粒转染COS-1细胞，经间接免疫荧光试验证明，上述单克隆抗体能与转染细胞发生特异反应。

The molecule of taxol is an extremely complex diterpene with a taxane ring structure fused with a four-membered oxetane ring, an ester side-chain at position C-13 and a benzoyl side-chain at the C-2 position Fig.

紫杉醇分子是一个极其复杂的四环二萜类化合物：一个紫杉烷环加一个四个元素组成的氧烷环，在C-2和C-13上还分别有一个苯氧基和酯基侧链。

Both hexanitrohexaazatricyclododecanedione and hexanitrohexaazatricyclotetradecanedifuroxan are the typical representatives of fused ring-nitroamine. 4, l0-Dinitro-2, 6, 8, 12-tetraoxadiazatetrocyclo [5.5.0.0^(5.9). 0^(3.11)] dodecane is the typical representative of cage nitroamine.

六硝基六氮杂三环十二烷二酮和六硝基六氮杂三环十四烷并双氧化呋咱是稠环硝胺化合物中的典型代表；4， 10-二硝基－2， 6， 8， 12-四氧杂－4， 10-二氮杂异伍兹烷是笼形硝胺化合物中的典型代表。

Dorsifixed A stamen in which the filament is fused to the back of the anther.

背着的：是一种雄蕊花药的着生方式，指花丝与花药在背部联合。

Inflorescence capitulate, subtended by 4 large showy petaloid bracts; fruit of each inflorescence fused, forming a compound drupaceous berry.

头状的花序，以4年被包着大艳丽苞片；每花序的果熔化，形成一种化合物核果状浆果。

Emperor Heraclius attempted to reconcile the monophysite bishops, who held that the human and divine natures in Christ were fused together to form a third, by offering in his ecthesis in 638 the view that Christ worked through a divine - human energy.

皇帝heraclius试图调和monophysite主教，谁认为，人类和神圣性，在基督里融合在一起，形成了三分之一，提供在他的ecthesis在638认为，基督的工作，通过神-人类能源。

Emperor Heraclius attempted to reconcile the monophysite bishops, who held that the human and divine natures in Christ were fused together to form a third, by offering in his ecthesis in 638 the view that Christ worked through a divine - human energy.

皇帝赫拉克留试图调和monophysite主教，谁认为，人类和神在基督天性融合在一起，形成了三分之一，提供的ecthesis的638认为，基督的工作通过一项神圣-人类能源。

Female flowers at base of inflorescences, larger than male ones, each bract-axil with only 1 female flower; pedicel short but distinct; calyx, rarely 2-sepaled, slightly fused at base; petals and disk absent; ovary 2-celled; ovules 1 per cell, smooth; styles short; stigmas 2, revolute, entire, eglandular.

雌花在花序的基部，比雄花大，每苞片腋只具1雌花；花梗短但是明显；花萼，很少2萼片，稍在基部愈合；花瓣和花盘无；子房2室；胚珠1 每室，平滑；花柱短柱头2，外卷，全缘，无腺体。

After decided on the concept, the designer had decided to create a masterwork that will have a great impact locally and internationally. The hotel is fused in between Far East and west, but also has its local character and perfect electivity.

在确定设计概念之后，设计师决定在当地创造一个震撼人心的酒店，它具有东方和西方融合的神韵，同时也含有原生地主题的设计元素和高明的折衷。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。