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Furfur was inoculated to the mycelial culture liquid medium and the rapeseed oil culture liquid medium, and then incubated in vibrator to induce the production of mycelial phase and yeast phase of M.

用全脂牛奶平板法、Folin-酚法、QuantiCleave蛋白酶试剂盒测定菌丝相及酵母相糠秕马拉色菌胞外蛋白酶的活力,并检测温度、pH值对蛋白酶活性的影响。

There are several kinds of Malassezia in the scales of PV patients and the M. globosa, M. restricta, M. sympodialis, M. furfur are the dominant strains.

PV皮屑中存在多种马拉色菌,球形马拉色菌、合轴马拉色菌和限制马拉色菌是PV患者皮损处的主要马拉色菌种。

Results: The autofluorescence of M. furfur and M. japonica standard trainsrespectively in 4th and 6th day are stronger than that in other days, andautofluorescence of colonies become weaker in an hour. Theautofluorescence of Malassezia colonies put in 3% glutaraldehyde, pH4.0HCL, 1280μg/mL and 64μg/mL fluconazol solution, 0.3% methylene blueseparately get stronger, and that in 10% KOH get stronger at beginning thenbecome weaker 2 days later. C. albicans, S. schenchii, T. rubrum, T.tonsurans, A. terreus, A. fumigatus cultured on SDA all haveautofluorescence, and which are weaker than that of Malassezia.

结果:糠秕马拉色菌和日本马拉色菌2株标准株的自发荧光强度分别在培养第4和8天时最强,菌落的自发荧光在1小时内逐渐减弱;3%戊二醛溶液、pH4.0盐酸溶液、1280μg/mL和64μg/mL氟康唑溶液、3%美蓝溶液处理后马拉色菌自发荧光都增强,10%氢氧化钾溶液中的马拉色菌在开始时荧光增强,2天后减弱;白念珠菌、申克孢子丝菌、红色毛癣菌、断发毛癣菌、土曲霉、烟曲霉等在培养条件下有自发荧光,但都较马拉色菌弱。

Furfur;②To study the difference between mycelial phase and yeast phase in the proteinase activity;③To study the effect factors of proteinase activity.Methods: The standard strain of M.

选用糠秕马拉色菌标准株,接种于菜籽油液体培养基和菌丝液体培养基中振荡培养,诱导糠秕马拉色菌菌丝相及酵母相生成,收集培养上清液。

To explore the possibility of early and rapid diagnosis of mycosisby detecting the fungal autofluorescence. Methods: To culture M. furfur and M. japonica standard strains on Dixonmedium at 32℃, and to study the autofluorescence of colonies in 2nd, 4th, 6th, 8th, 10th day under Laser Scanning Confocal Microscopy (LeicaTCS-SP2 LSCM). To study the variation of autofluorescence whenMalassezia colonies were put in 3% glutaraldehyde, pH4.0 HCL, 10% KOH, fluconazol solution, 0.3% methylene blue separately. To detect theautofluorescence of C. albicans, S. schenchii, T. rubrum, T. tonsurans, A.terreus, A. fumigatus cultured on Sabouraud dextrose agar.

选用糠秕马拉色菌和日本马拉色菌2株标准菌,接种在Dixon培养基32℃培养,分别在培养的第2、4、6、8、10天挑取菌落放在TCS-SP2型激光扫描共聚焦显微镜(Leica TCS-SP2 LSCM)下观察、测定其自发荧光;分别用3%戊二醛溶液、pH4.0盐酸溶液、10%氢氧化钾溶液、1280μg/mL和64μg/mL氟康唑溶液、0.3%美蓝溶液处理后观察马拉色菌自发荧光的变化;选择白念珠菌、申克孢子丝菌、红色毛癣菌、断发毛癣菌、土曲霉、烟曲霉等用沙堡葡萄糖琼脂培养后观察其自发荧光的特点。

The effect of temperature, pH and proteinase inhibitor on this assay were also evaluated.

furfur正常皮肤分离株蛋白酶活性,并检测温度、pH值和蛋白酶抑制剂对蛋白酶活性的影响。

The results indicate that fungi, especially M.furfur, are more frequently isolated in the lesions and uninvolved skin of psoriatic patients than those in healthy controls.

真菌特别是糠秕孢子菌在银屑病患者的皮损处和正常皮肤上都较正常人高,对银屑病的发病有一定影响。

The stereochemical structures of two new compounds, 5-(3-chlorophenyl)-2-furfur-aldehyde-N-4-methylphenyloxyacetylhydrazone and 5-(3-chlorophenyl)-2-furfuraldehyde-N-4-nitrophenyloxyacetylhydrazone, were determined by DPFGSE-NOE experiments performed in DMSO-d\-6 at 25 ℃ and variable-temperature ~1H NMR The ~1H chemical shifts of the compounds were completely assigned at 25 ℃ and 90

利用变温实验和DPFGSE NOE实验,确定了 5 (3 氯苯基) 2 呋喃甲醛 N 4 甲基苯氧乙酰腙和5 (3 氯苯基) 2 呋喃甲醛 N 4 硝基苯氧乙酰腙两个新化合物25 ℃时在二甲基亚砜中的空间构型,并对这两个新化合物在 25 ℃和 90 ℃时的质子的化学位移进行了全归属。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?