查询词典 fungal

Abstract］ Objective: To verify if a DNA fragment we obtained from Pythium guiyangense Su before is the ORF of a subtilisin-like protease (Pr1) of P. guiyangense, a fungal pathogen of mosquitoes.

贵阳腐霉是由贵阳医学院苏晓庆教授分离得到的一株具有自主知识产权的可以高效地、特异地杀灭蚊幼虫的水生丝状真菌，可以有效切断经蚊虫传播疾病的途径［1］。

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育，MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用；RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1)，以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况；Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达，以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用；免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达；激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位，并观察真菌刺激后人角膜上皮细胞骨架的变化；流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变；光学显微镜观察真菌与人角膜上皮细胞黏附数量，记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后，细胞超微结构的改变。

This article reviewed the research progress of anti-bacterial and anti-fungal activity of volatile oil from kinds of aromatic Chinese herbs including aromatic herbs for relieving exterior syndrome, aromatic herbs for clearing heat syndrome, aromatic herbs for resolving dampness, aromatic herbs for resuscitation and aromatic herbs for warming and dredging meridians, and also reviewed our antibacterial experimental results of aetherolea from ramulus cinnamomi, Herba Schizonepetae, Herba Moslae,et al, which may provide new ideas for the development of volatile oil preparations.

本文综述了多种来源于芳香解表类、芳香清热类、芳香化湿类、芳香开窍类和芳香温通类的挥发油在抗细菌和抗真菌作用方面的研究进展，结合笔者对桂枝、荆芥、香薷等中药挥发油抗菌实验研究结果，为挥发油抗菌制剂的开发提供新思路。

Although cholestasis of sepsis is often associated with gram-nagetive bacterial infection,it also occurs with a diverse array of other organisms,including Gram-positive bacteria,rickettsial,parasitic and fungal infections.in one study of bacteraemic patients with positive blood cultures only 34% had bilirubin levels greater than or equal to 2.0mg/dl.moreover,in clinical practice the frequency and extent of liver enzyme abnormalities in bacteraemic patients may be far greater than the occurrence of hyperbilirubinaemia.cholestasis appears to be a marker for the severity of sepsis,and prolonged and unresolved cholestasis carries a high mortality.

虽然败血症（sepsis引发的胆汁郁积往往与革兰氏阴性菌（gram-negative bacterial，原文negative瓶错了）感染，它也可能伴随各种各样其他的有机体，包括革兰氏阳性菌（Gram-positive bacteria），立克次体属微生物，寄生虫和真菌感染。一个对菌血症（bacteremic，原文中拼错了）的阳性患者的血液培养分析研究发现其中只有34％的胆红素水平大于或等于2.0mg/dl。而且，在临床实践中的血症患者肝酶异常的发生频率和严重程度可能远远大于高胆红素血症（hyperbilirubinemia，原文中拼错了）。胆汁郁积似乎是一个患有严重性败血症的标志，而且长期的和没有根治的胆汁郁积有很高的死亡率。

Objective To discuss the clinical characteristics, diagnostic outlines and surgical therapeutic principle of noninvasive fungal nasal sinuitis.

目的 探讨非侵袭性真菌性鼻窦炎的临床特征、诊断要点及手术治疗原则。

Multilayer amniotic membrane transplantation plus subconjunctival injection of autologous serum treatment of fungal corneal ulcer is good.

多层羊膜移植加自体血清结膜下注射治疗真菌性角膜溃疡疗效良好。

Research was conducted on the inhibitory effect of the volatile oil on several plant pathogenic fungi. Fungal colony diameter was measured in the inhibition experiments which indicated the presence of substances with antifungal activity in the roots of Tagetes patula. These results provide a valuable reference for development of fungicides.

对挥发油对几种植物病原菌的抑菌活性作了研究，抑菌实验采用菌落直径法，研究结果表明，万寿菊根的挥发油中含有抑菌活性物质，该实验可为开发新的杀菌剂提供有价值的参考。

Tilletia controversa Kühn, one of the important international quarantine fungal pathogens, causes wheat dwarf bunt disease which distribute in America, Europ, Northern Africa and West Asia, especially in 7 Northwest states of USA.

小麦矮腥黑穗病(Wheat dwarf bunt disease， DB)是麦类黑穗病中危害最大、极难防治的国内外检疫性病害之一，是由小麦矮腥黑穗病菌(Tilletia controversa Kühn， TCK)引起的。主要分布在美洲、欧洲、北非和西亚，尤其是美国西北部7州。

Were dermatophytes (92.19%), 57 were Trichophyton rubrum (89.06%);5 yeast (7.81%), Candida albicans was the most; mold and mixed fungal infection were never isolated.

共分离出致病真菌64株，皮肤癣菌59株(92.19%)，其中红色毛癣菌57株(89.06%)；酵母菌5株(7.81%)，白念珠菌最常见。

Results Bamboo Vinegar had obvious anti- bacteria and fungal actions for the trichophyton rubrum .Trichophyton actions,and canida albicans,cryptococcus neoformans,aspergillusfumigatus.

目前国内对竹醋液抗真菌作用的研究相对较多，而对抗细菌的报道较少，主要有大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌，对这三种细菌的MIC和MBC的测定结果也不一致［3，5］。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。