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Maybe you thought Battletoads was a game full of amphibians, but it is actually full of bugs.

也许你以为Battletoads是一个充满游戏的两栖动物，但它实际上是没有错误的。

Schumacher has previously baulked at the idea of returning to F1 full-time, but with Massa looking likely to return when he is back to full fitness, and Ferrari in need of a top-line driver, the opportunity is one that has attracted him.

马萨看起来似乎会在痊愈后的回归，因此舒马赫显然有所保留，但法拉利需要一名顶尖车手，这个机会十分吸引他。

Hello! For TOSHIBA / SHARP agents of a branch in mainland China, established in June 2003, the main element in the electronic device in the agents and the development of electronic technology to promote business; operating a full range of Toshiba TOSHIBA, SHARP SHARP; Yamaha YAMAHA, USA Bonn BOURNS, Taiwan GTM, and Shanghai Belling BELLING full range of electronic products.

为TOSHIBA/SHARP的一级代理商在大陆的分支机构，成立2003年6月，主在从事电子元器代理和电子技术的开发，推广的业务；经营全系列东芝TOSHIBA 、夏普SHARP；雅马哈YAMAHA ，美国柏恩BOURNS ，臺湾GTM ，及上海贝岭电子BELLING 全系列產品。

Big Rock Candy Mountain 】◎One evening as the sun went down And the jungle fire was burning Down the track came a hobo hikin' And he said, boys, I'm not turning I'm headed for a land that's far away Beside the crystal fountains ◎So come with me, we'll go and see the big rock candy mountains In the big rock candy mountains There's a land that's fair and bright Where the handouts grow on bushes And you sleep out every night Where the boxcars all are empty And the sun shines every day On the birds and the bees and the cigarette trees The lemonade springs where the bluebird sings In the big rock candy mountains ◎In the big rock candy mountains All the cops have wooden legs And the bulldogs all have rubber teeth And the hens lay soft-boiled eggs The farmers' trees are full of fruit And the barns are full of hay Oh, I'm bound to go where there ain't no snow Where the rain don't fall,the wind don't blow In the big rock candy mountains ◎In the big rock candy mountains You never change your socks And the little streams of alcohol Come a-trickling down the rocks The brakemen have to tip their hats And the railroad bulls are blind There's a lake of stew and of whiskey, too You can paddle all around 'em in a big canoe In the big rock candy mountains...

在那天傍晚日落的时候如同野火在林中蔓延沿着那小路走来一位旅行者他说，男孩们，我决不会回头我一定要到那远方的国度或许就在水晶泉的旁边所以跟我远走高飞吧，我们终将到达那巨石糖果山在巨石糖果山里有个公正明亮的国度灌木从中生长着必需品你可日日在外露宿在那里箱车都是空的每一天都那么晴朗鸟儿和蜜蜂在烟草树周围徘徊蓝鸟在柠檬水的喷泉中放歌在在巨石糖果山里在巨石糖果山里警察的腿都是木桩子公狗的牙都是橡胶的母鸡们生的是荷包蛋果农的树上果实遍布干草也会堆满了马厩 Oh，我一定要去那个没有冰雪的地方雨水不会冲击，狂风不会怒吼在巨石糖果山里在巨石糖果山里你可以不换袜子还有淌着酒的河流如果从岩石上滑下来修补匠们就的去补他们的帽子会有挡在铁轨上的瞎公牛威士忌和炖肉的小湖你可以把它们都搬到船上在巨石糖果山里在巨石糖果山里监狱是用罐头围成即使你被关进去也可以马上再出来这里没有短柄铲子没有斧子，锯子或是镐你我可终日呼呼大睡蠢人才会去工作在巨石糖果山里我将看到你们全都会堕落在巨石糖果山里

Article 34 Entities operating and using such special equipment as elevators, passenger cableways and large entertainment facilities that provide services to the public shall set up the agency of special equipment safety administration or equip full-time safety administration personnel; entities using other special equipment shall set up the agency of special equipment safety administration or equip full-time or part-time safety administration personnel according to the circumstances.

第三十四条电梯、客运索道、大型游乐设施等为公众提供服务的特种设备运营使用单位，应当设置特种设备安全管理机构或者配备专职的安全管理人员；其他特种设备使用单位，应当根据情况设置特种设备安全管理机构或者配备专职、兼职的安全管理人员。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛，三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

This English certificate examination system, a set of full and independent certificate system consisting of Listening, Speaking, Reading and Writing, was developed in 1999 by the University of Cambridge Local Examinations Syndicate on the basis of full market research of English Learning in over 100 non-English speaking countries. The first CELS test was administered on May 2002 throughout the world.

CELS证书考试体系，是由剑桥大学考试委员会，在对全世界一百多个非英语国家英语学习市场需求，进行充分和全面研究的基础上，为16岁以上英语为非母语者，在1999年设计建立的一套具备听、说、读、写各单项技能组成的完整和独立的英语证书考试体系，首次考试业已于2002年5月在世界各地进行。

In this free nature, why some iters were not chuffed, because of either occupational habits or mental exhaustion, or because of the cities that are full of material but also full of more and more people and that can't give people good mood.

在这轻松的自然里，为何一些 ITer 的心情似乎并没有舒展和释放，是职业的习惯，还是心理的疲惫，还是物博人更博的城市不能给人释怀。

This marks the first Pixar release that doesn't include both the widescreen and full-screen versions in the same DVD set, which was a great bargaining chip for those cinephiles who still want a full-frame presentation for other family members.

这标志著首次发布皮克斯不包括宽屏和全屏的版本在同一DVD集，这是一个伟大的筹码为那些cinephiles谁也想一全画幅介绍其他家庭成员。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。