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In the research, one kind of guzmania species named ostara was employed to be as material, and the full-length cDNA plasmid library of floral organ was constructed successfully by using SMART technology. The library had 3×10^6 original titer and more than 1 kb insert fragments. After 5'EST sequencing from 2004 positive clone chosen at random and clustering analyse, 1 758 high-quality sequences and 1 365 unigenes including 175 contigs and 1 195 singlets were obtained. These unigenes had 1 283 valid ORFs. COG analysis showed that those proteins coded by EST sequences were divided into 22 classes. Through blast analysis, full length or part cDNA of some genes controlling flower color, development of floral organs, florescence regulation and other breeding value genes were obtained.

本研究以擎天凤梨属品种Ostara为材料,采用SMART技术成功构建了擎天凤梨花器官的质粒型全长cDNA文库,初始文库滴度为3×10^6,插入片段平均长度大于1kb;随机挑取2004个阳性克隆进行5'EST测序,获得高质量序列1758条,经拼接获得1365条单基因簇,其中跨叠群175个和单条序列1195个;经ORF寻找共获得有效ORF1283条;经COG分析EST序列编码的蛋白质被分为22类;经Blast分析后,获得一批花色、花器官发育、花期调控以及其它育种价值基因(包括cDNA全长或片段)。

In the vertical hydrodynamical zonation, the saturated zone is full of karst ground-water, while the aerated zone is full of air.

在垂直水动力分带中,下部饱水带中的地下空间充满喀斯特地下水,而包气带中的地下空间中绝大部分是空气。

The pattern of lunar cycle can be classified into three strategies:(1) Planulation of S. hystrix and P. damicornis showed clear lunar periodicity and the peak of larval release shifted with different months. The peak of planulation occurred around the full moon in winter then shifted gradually to the first quarter moon phase in summer. The phase shift of larval release may be related to the seasonal variation of seawater temperature and the sudden temperature drops caused by upwelling.(2) Planulation of S. pistillata showed clear lunar periodicity and the peak of larval release occurred around the full moon.

在月周期方面可分成三种生殖策略:(1)尖枝列孔珊瑚与细枝鹿角珊瑚释放幼生不但有明显的月周期,而且释放幼生的高峰会随著月份而移动,由冬季至夏季期间,高峰从满月前后转变至上弦月前后;再由夏季至冬季期间,高峰从上弦月前后转变至满月前后,而此变动形成一个完整的年周期,原因可能与海水温度影响配子发育和胚胎速度与夏季期间涌升流所造成的降温有关。

Our customer is a crowd fill deep love to the life,can welcome indomitably facing the life in setback difficultly on;Will have full self-confident, the full enthusiasm ,for relentlessly courageously strives for success ideally deeply loves the life, and puts on is regarding as individual disposition and the life style expression male and the female.

我们的客户是一群对生活充满热爱,面对生活中的挫折能够不屈不挠迎难而上;具有充分的自信、饱满的热情、为理想坚持不懈地奋勇拼搏热爱生活、并将穿着视为个人性格和生活风格的表达的男性和女性。

Our main brand agent has: a full range of Capacitive Products Korea, Japan and aluminum electrolytic capacitors OS-CON/POSCAP, EMERAL TECH Power Module DC-DC (with vicor, Interpoint compatible) high-performance, high efficiency, high reliability, such as a full range of Products.

我们主要代理的品牌有:韩国全系列电容产品,日本铝电解电容及OS-CON/POSCAP, EMERAL TECH电源模块DC-DC(与vicor、Interpoint兼容)高性能、高效率、高可靠性等全系列产品。

Jin Zhixiong bully battle: Jin Yang basin, the place of an invaluably, the advanced ore of contain and advanced material are full of infinite temptation to all fierce forest personages, male, female when two cliques and tripartite of day demon army group make here not have again however to the contention here, do not engrave be full of the crisis, who can become the winner here?

金之雄霸战:金阳盆地,一个无法评价的地方,蕴藏的高级矿石和高级材料对所有武林人士都充满无限的诱惑,乾、坤两派和天魔军团三方对这里的争夺却又令这里无时不刻充满危机,谁能成为这里的胜利者?

Dot = Full Group MOBs. Killable by a full group

3个圆点=小组的怪物要2到4个玩家队伍挑战。

Mr. Knoller helped earn President George W. Bush his reputation for frequent trips outside Washington, counting that over two terms Mr. Bush made 77 trips to his ranch in Texas, spanning all or part of 490 days; spent 43 full or partial days at the family compound in Kennebunkport, Maine, and 487 full or partial days at the presidential retreat in Camp David, Md.

克内勒的统计使得小布什总统有了经常离开华盛顿出游在外的名声:根据他的记录,布什在两届总统任期内,去了德克萨斯自家的牧场77次,待了490个全天或非全天,在缅因州肯纳邦克波特的家族庄园里待了43个全天或非全天,在马里兰州的总统度假地戴维营待了487个全天或非全天。

At aim of getting the full 235 bp gene, five 50~70 bp model-based primers were first synthesized by chemical method followed by 3-step PCR amplification in an effort to ligate them into full gene.

为了得到目的肽全基因,首先合成5条50~70 bp互为模板引物的DNA片段,再通过PCR扩增将5条片段连接成全基因。

The method causes bandicoot full head to be short of hematic model through 4 arterial ligate, applied NO electrode is short of blood to reach in full head to area of bandicoot sea horse again change of chroma of the NO below perfusion condition undertakes real time detects.

梗概: 目的探索大鼠海马区在全脑缺血及再灌注状态下NO浓度变化的动力学。

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Young Dumb And Full Of It
Full Moon Song
Full Circle
8 Full Hours Of Sleep
Full Of Fire
Living In A House Full Of Love
Amazing Because It Is (Full Version)
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Head Full Of Doubt / Road Full Of Promise
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推荐网络例句

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作:对伸臂位于顶部的单层框架—筒体模型进行分析,提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。

Its advantage is that it can be used in smaller units.

其优点在于可以在较小的单位中应用。