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The Omp25 gene of wapiti's Brucella abortus was amplified by RT-PCR and sequenced, Then the interesting gene was subcloned into pET28a for expression. The results showed that the full length of Omp25 gene was 642bp, which encoded 213 amino acids.

运用RT-PCR技术从马鹿布氏杆菌分离株中扩增出Omp25基因，将其克隆入pMD18-T中进行核苷酸序列测定和遗传变异分析，并将目的基因亚克隆到大肠杆菌表达载体pET28a中诱导表达。

And this I pray, that your love may abound yet more and more in full knowledge and all discernment

1：9 我所祷告的，就是要你们的爱，在充足的知识并一切的辨识上，多而又多的洋溢

The coming new age will abound in opportunities, but it will be likewise full of challenges.

即将到来的新时代将充满机遇，同样也充满挑战。

Responsible for the above-mentioned company's products in China a full-fledged agents and sales services.

负责上述公司的产品在中国地区的全权代理和销售服务。

Long time being away of my blog-full of grass, it seems that the original meaning of blog is getting more information from different way and write extensively, back to my blog, just for fun and selfish abreaction, but feeling not bad.

又是很久才来光顾一下我的长草的blog，呵呵，看博客的本意原来是有博览众象之意，再回头看看自己的blog完全是自己的心情写照，不过也好，有的发泄也不失为好事。

This is an abridgement of the widely acclaimed full-length edition of Birds, published in 1995, which was the first comprehensive edition in any language.

这是广泛流传的全本《鸟》的节本，全本在1995年刊行，是在所有语言中最全面的版本。

It'll be a great idea to release a full coverage DVD version of this fantastic show without abridgement and Ad interruption.

弄个无广告的完整版DVD可真是好主意。

And his voice was abruptly full of ancient grief.

他的声音突然充满了远古的悲伤。

And Absalom dwelt in Jerusalem for two full years and did not see the king's face.

14：28 押沙龙住在耶路撒冷足有二年，没有见王的面。

With power reduced to full military, maximum climb rate drops to 47307ft/min with an absolute ceiling of 53430ft.

藉由力量减少到充足的军队，最大值攀登对和 53430 ft 的 absolute 天花板的 47307 ft/最小的比率下降。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。