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Methods We isolated and cultured the MSCs, then induced MSCs into osteoblast and checked the osteoblast by RT-PCR; the conditioned medium in different concentration was prepared containing diphosphonate or assemble flavone of drynaria rhizome; with the conditioned medium the osteoblast was cultured; then the growth curve was analyzed and the cytogene (collagenase col Ⅰ) col Ⅰ and ALP expression was detected.

使用诱导方法作用于骨髓基质干细胞，使之转化为成骨细胞；用RT-PCR检验成骨细胞；不同浓度的二磷酸盐或骨碎补总黄酮配制成条件培养基，作用于成骨细胞，并分析生长曲线，分析细胞基因碱性磷酸酶和胶原酶Ⅰ(collagenase col Ⅰ)表达情况。

The governing equation of in-plane vibration of cable-restraint system is derived by means of D'Alembert principle, and then those partial differential equations are transformed into a set of ordinary differential equations by Garlerkin method. The method of Runge-Kutta integration is applied to solve the equation. The simulation analysis is made to prove that this vibration control has obvious damping effects and then the influence of cable tension, support mass, natural frequency and spring stiffness on the damping are discussed. Eventually, the approximate analytic solution of the optimum damping parameter is obtained to provide a simple and effective reference and design method for the engineers.

通过D'Alembert原理建立拉索－弹性约束系统振动方程，通过Galerkin方法将偏微分方程转化为常微分方程，应用龙格－库塔积分法求解方程；经过仿真分析，验证了该振动控制具有明显的减振效果，并且讨论了初始拉力、支座质量、振动频率及弹簧刚度对减振效果的影响；最后给出了计算最优阻尼参数的近似解析式，为工程师提供了简便有效的参考依据及设计方法。

Later on he cuts Thakur's hand and then Thakur hires two thiefs (Jay and Biru both were great friends) to confront the dacoit and then begins the war to catch and kill Gabbar Singh.

后来他就削减thakur的手，然后thakur聘请两名瞩目，以对抗名牌，然后开始战争捕捉并杀死潜入印度。

Here's an example: You hear DAH DIT DIT which is a dash then dot then dot.

下面是一个例子：你听到瓦达赫抽取抽取这是一个破折号然后点然后点。

He then warns Edmund, saying,"I'll do my damnedest to make you fail," but then he admits, You're all I've got left.

"然后，他警告道，他说："我会尽我的damnedest让你失败了，"但他承认，"你是所有我得留下。

Since then, the guiding principle for managers everywhere has been to gather up whatever cash they can find, and then do their damnedest to keep as much of it as possible for as long as possible.

自从那时起，经理们的最高原则就是到他们可以找到资金的地方去寻找资金，并竭尽全力争取更多的资金，争取更长的资金占用时间。

University of Central Florida biomedical scientists led by Professor Henry Daniell found insulin might someday be grown in genetically modified plants and then be used to prevent diabetes before symptoms appear or to treat the disease in its later stagesDaniell's research team genetically engineered tobacco plants with the insulin gene and then administered freeze-dried plant cells to five-week-old diabetic mice as a powder for eight weeks.

在弗罗里达中央大学，由Henry Daniell教授带领的生物医学学家们发现胰岛素可以在转基因植物中生产，以用来在症状出现之前预防糖尿病或在疾病晚期治疗糖尿病。Daniel的研究小组将胰岛素基因通过基因工程技术重组到烟草植物之中，再将经过冷冻干燥过的植物细胞用作粉剂喂食五周大的糖尿病鼠8周。

Then they had spoken with Miss Frandsen; she knew all about the ballet, and at one time, in Grand-mother's younger days, she had been the most beauti－ful danseuse at the theater; she had danced goddesses and princesses, had been cheered and applauded wher－ever she had gone; but then she had grown older—weall do—and so no longer had she been given principal parts; she'd had to dance behind the younger ones;and when finally her dancing days had come to an end, she had become a wardrobe woman and dressed the others as goddesses and princesses.

因此她们就又去和佛兰生小姐商量。这位老小姐懂得有关芭蕾舞的一切事情，因为在祖母还很年轻的那些日子里，她曾经一度也是舞台上的一位漂亮的舞蹈家。她扮演过女神和公主的角色，她每到一地都受到欢迎和敬意。不过后来她的年纪大了——我们都会如此——再没有什么主要的角色给她演了，她只能在一些年轻人的后面跳舞，最后她只得退出舞台，做些化妆工作——为那些扮女神和公主的角色化妆。

Subsequently, he held the position of Head of Section for international switching at the Telecommunications Centre at Darmstadt. He then became project leader and Head of Section for the digitalization of the telephone network and the introduction of ISDN in the former Federal Ministry of Posts and Telecommunications in Bonn and then Head of Department back at the FTZ in Darmstadt for telephone and ISDN Technology, Planning, Operation, Testing and Services.

后来，他担任位于达姆施塔特的电信中心国际配电系统部负责人；随后担任项目负责人和电话网络数字化部门负责人，并成为前波恩邮电和电信部ISDN的引入者，后来在达姆施塔特的FTZ支持部门领导电话和ISDN技术、策划、运营、测试及服务等工作。

Since had own starship, the kind of person robot then starts in the summit sky execution patrol mission, moreover the arrogance is more rampant, has created the very great difficulty to the humanity police power's further bombing, was good jumbles together in the hybrid area senior official with 黒 area senior official black Germany graceful this time has brought the massive long-distance weapon delivery, therefore then stay up till dawn carried on the rug type saturation bombing to the Moon crater base, caused in the crater the kind of person robot not to dare to appear easily, also their flight vehicle after shot down the dozens of frames does not have to remove into in the base to hide.

自从有了自己的星际飞行器，类人机器人便开始在山顶上空执行巡逻任务，而且气焰更加嚣张起来，对人类警察部队的进一步轰炸造成了很大难度，好在混血区长官混为一谈和黒区长官黑德曼这次又带来了大量远程发射武器，于是便日夜不停地对月球环形山基地进行地毯式饱和轰炸，使环形山内类人机器人不敢轻易露头，它们的飞行器在被击落数十架之后也不得撤入基地内隐蔽起来。

"Please accept this talisman as a token of our thanks."

请收下这个护身符以表达我们的感谢。

If I have that magic, I will be more acceptable in the society.

如果我拥有那种魔法，我将更加被这个社会所容纳。