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The first thing to see, looking away over the water, was a kind of dull line -- that was the woods on t'other side; you couldn't make nothing else out; then a pale place in the sky; then more paleness spreading around; then the river softened up away off, and warn't black any more, but gray; you could see little dark spots drifting along ever so far away -- trading scows, and such things; and long black streaks -- rafts; sometimes you could hear a sweep screaking; or jumbled up voices, it was so still, and sounds come so far; and by and by you could see a streak on the water which you know by the look of the streak that there's a snag there in a swift current which breaks on it and makes that streak look that way; and you see the mist curl up off of the water, and the east reddens up, and the river, and you make out a log-cabin in the edge of the woods, away on the bank on t'other side of the river, being a woodyard, likely, and piled by them cheats so you can throw a dog through it anywheres; then the nice breeze springs up, and comes fanning you from over there, so cool and fresh and sweet to smell on account of the woods and the flowers; but sometimes not that way, because they've left dead fish laying around, gars and such, and they do get pretty rank; and next you've got the full day, and everything smiling in the sun, and the song-birds just going it!

往 水面上望去，首先看到的是灰蒙蒙的一条线——那是河对岸的树林子——别的便什么也看不清——接着是天空中有一点儿鱼肚白；然后鱼肚白多了些，逐渐朝四周散开去；接下来，远处河水的颜色淡了些，不是那么黑了，而是灰灰的了。更远处，可以看到小小的黑点子在漂过来——那是些载货的驳船之类。还有黑黑的一长条——那是木筏子。有时能听到长桨吱吱地响，或者一些杂音。四周这么寂静，声音又来自很远的远方。过了一会儿，你看到一道水纹。凭了水纹的模样，你知道那里有一块礁石，急流朝着它冲过去，流水飞溅，成了这个模样。你看到，雾气袅袅上升，离开水面，东方红了起来，河面红了起来。你可以看到对岸河边树林子边上一处原木搭成的小屋，那可能是一个木材场，在那里堆着的一堆堆木材，中间却是空的，容得狗钻来钻去，为了能叫人家上当①。然后微风轻拂，从河上一阵阵吹来，那么凉凉的，那么清新，闻起来那么甜美，这是全凭了那些树林子和那些鲜花的缘故。可有时候也并非全是如此美妙。

The business model of this system is: The purchasing agent who operates the authority log-in www.gdmec.net, beginning direct purchase, this system offers two to purchase the entry , which are establish inquiry sheet manually and establish inquiry sheet in Synchronism, after entering procedure of purchasing , choosing the products from supplier's catalogue of purchasing, confirming inquiry sheet that is salesman of supplier that send , then filling in the basic information of the inquiry sheet, then sending the inquiry sheet; Then fill in the basic information of the inquiry sheet , then send the inquiry sheet; The supplier's salesman receives after the inquiry sheet, and then the products that will buy are offered to purchasing, purchase and then get quotation sheet can carry on counter-offer and carry on the rate of exchange to different quotation of supplier to receive, supplier offer and purchase square course of counter-offer not to can circulate, satisfied with quotation of supplier until purchase, the purchasing agent chooses to offer a satisfactory supplier and carry on the fixed price , turn into the order, the supplier receives after the order wanting to provide and respond, after accepting the order, should purchase the procedure and end .

本系统的业务模型为：具有操作权限的采购员登录国电物资商务网，进行直接采购，本系统提供两个采购入口，分别为手动创建询价单和同步创建询价单，在进入采购流程后，从供应商采购目录中选择产品，并确定该询价单要发送的供应商的销售员，接着填写询价单的基本信息，然后发送询价单；供应商的销售员接收到询价单后，针对采购方要购买的产品进行报价，采购方接收到报价单后可以进行还价和对不同的供应商的报价进行比价，供应商报价和采购方还价的过程可以循环，直到采购方满意供应商的报价为止，采购员选择报价满意的供应商进行定价，生成订单，供应商接收到订单后要给出回应，接受订单后，该采购流程宣告结束。

In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2～0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%～80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0～0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各种纯化方法，将离子交换层析、亲和层析和疏水层析三种分离纯化法相结合，建立了激肽释放酶、胰蛋白酶和糜蛋白酶三酶的联产工艺：胰酶用pH4.5醋酸缓冲溶液提取后，粗提液直接上DEAE-琼脂糖快胶柱吸附平衡缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5，洗脱缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5)含0.2～0.35mol／LNaCl分两步洗脱，收集激肽释放酶的洗脱峰；DEAE-琼脂糖快胶的未吸附液上亲和层析柱分批吸附平衡缓冲液：0.01mol／LTris-HCl缓冲液(pH7.5，洗脱液：0.5mol／LNaCl溶液，一次洗脱，收集胰蛋白酶洗脱峰；最后，亲和层析未吸附液用30％～80％硫酸铵分级盐析处理，沉淀溶解后用上苯基—琼脂糖快胶吸附平衡缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5含1mol／L(NH_4)_2SO_4，洗脱缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5)含0～0.6mol／L(NH_4)_2SO_4，分两步洗脱，收集糜蛋白酶的洗脱峰。

So saying, with dispatchful looks in haste She turns, on hospitable thoughts intent What choice to chuse for delicacie best, What order, so contriv'd as not to mix Tastes, not well joynd, inelegant, but bring [ 335 ] Taste after taste upheld with kindliest change, Bestirs her then, and from each tender stalk Whatever Earth all-bearing Mother yields In India East or West, or middle shoare In Pontus or the Punic Coast, or where [ 340 ] Alcinous reign'd, fruit of all kindes, in coate, Rough, or smooth rin'd, or bearded husk, or shell She gathers, Tribute large, and on the board Heaps with unsparing hand; for drink the Grape She crushes, inoffensive moust, and meathes [ 345 ] From many a berrie, and from sweet kernels prest She tempers dulcet creams, nor these to hold Wants her fit vessels pure, then strews the ground With Rose and Odours from the shrub unfum'd.

Neerer his presence Adam though not awd， Yet with submiss approach and reverence meek， As to a superior Nature， bowing low， [ 360 ] 她一边说着，一边带着急迫的神情，急速转身，一心想着招待的事，怎样精选最上等最可口的东西，风味若不调和，便会变得粗劣，务必要按照自然的变化而加调味，一样接着一样，有条不紊，引人入胜；于是忙着去采集各种的果品，从大地母亲所生产的万汇中千挑万选，无论东西印度、地中海滨各国、本都、布匿沿岸或阿西诺斯国中的，样样都有，结在柔枝上的，有果皮光滑的，粗糙的，果壳带须的，包在荚里的，她都不吝惜地采摘，堆积在桌子上。

It analyses the essential factors, structure, environment as well as functions of high-tech corporation system and compares the similarities and differences between high-tech corporation system and traditional corporation system; it analyses characteristics in each stage of high-tech corporation lifecycle as well as management roots which lead to the declination of high-tech corporation and researches into the similarities and differences between high-tech corporation lifecycle and other lifecycles, including traditional corporation lifecycle, industry lifecycle, product lifecycle and ecology lifecycle; it studies functional coupling in each stage of high-tech corporation lifecycle and puts forward ways to realize internal coupling of high-tech corporation system and external environment coupling; based on fundamental concepts of dissipative structure, it summarizes six inherent laws of system evolution and on the basis of those laws it advances six principles of high-tech corporation lifecycle management, it poses four sequential parameters of high-tech corporation system, analyses coordination and competition between them and then it advanced four principles of high-tech corporation management; it researches into various cycles in high-tech corporation and puts forward five measures of high-tech corporation management from the point of hypercycle theory; it studies the relations between carious innovation of high-tech corporation innovation system and sets up models of innovation motive forces and resistances, after that it analyses the alternation laws of innovation motive forces and resistances in each stage of high-tech corporation lifecycle, puts forward some measures to strengthen motive forces and lessen resistances and then from the angle of dissipative structure theory, it researches into relevant questions of high-tech corporation innovation management; it studies the location, identification, cultivation, cultivation and protection of high-tech corporation core competence and researches into the key elements of core competence management in each stage of lifecycle and then from the angle of synergetic, it discusses several questions of high-tech corporation core competence management; it studies seven common characteristics of successful high-tech corporation culture and puts forward the ways and measures to cultivate and innovate high-tech corporation culture after that it researches into the characteristics of corporation culture in each stage of lifecycle and discusses relevant questions of high-tech corporation culture management from the point of hypercycle theory; it studies interplay of high-tech corporation system and environment and researches into the development and management of high-tech corporation ecology system after that it analyses the construction and disintegration of high-tech corporation strategic alliance.

剖析了高新技术企业系统的要素、结构、环境与功能，比较了高新技术企业系统与一般企业系统的异同；剖析了高新技术企业在生命周期各阶段的特点及衰退的管理根源，探讨了高新技术企业生命周期与一般企业生命周期、产业生命周期，产品生命周期和生物生命周期的异同；研究了高新技术企业生命周期各阶段的功能耦合，提出了实现高新技术企业系统内部耦合及系统与外部环境耦合的途径；以耗散结构理论的基本概念为基础，总结归纳出系统演化的六大内在规律，据此提出了高新技术企业生命周期管理的六个原则；提出了高新技术企业系统的四个序参量，剖析了生命周期各阶段序参量的协同与竞争，进而提出了高新技术企业管理的四大原则；研究了高新技术企业中的各种循环，从超循环理论的角度提出了高新技术企业管理的五大措施；研究了高新技术企业创新系统中各种创新的关系，建立了创新动力和创新阻力模型，剖析了生命周期各阶段创新动力与阻力的变化规律，提出了增强创新动力与减小创新阻力的措施，从耗散结构理论的角度研究了高新技术企业创新管理的相关问题；研究了高新技术企业核心竞争力的定位、识别、培养及保护，剖析了生命周期各阶段核心竞争力管理的要点，从协同理论的角度研究了高新技术企业的核心竞争力管理的几个问题；剖析了成功的高新技术企业文化的七个共同特点，提出了培养和创新高新技术企业文化的途径及措施，研究了生命周期各阶段企业文化的特征，从超循环理论的角度探讨了高新技术企业文化管理的相关问题；研究了高新技术企业系统与环境的交互作用，探讨了高新技术企业生态系统的开拓与管理，剖析了高新技术企业战略联盟的组建与解体。

Itanalyses the essential factors, structure, environment as well as functions of high-techcorporation system and compares the similarities and differences between high-tech corporationsystem and traditional corporation system; it analyses characteristics in each stage of-high-techcorporation lifecycle as well as management roots which lead to the declination of high-techcorporation and researches into the similarities and differences between high-tech corporationlifecycle and other lifecycles, including traditional corporation lifecycle, industry lifecycle,product lifecycle and ecology lifecycle; it studies functional coupling in each stage of high-techcorporation lifecycle and puts forward ways to realize internal coupling of high-tech corporationsystem and external environment coupling; based on fundamental concepts of dissipativestructure, it summarizes six inherent laws of system evolution and on the basis of those laws itadvances six principles of high-tech corporation lifecycle management; it poses four sequentialparameters of high-tech corporation system, analyses coordinfition and competition between themand then it advanced four principles of high-tech corporation management; it researches intovarious cycles in high-tech corporation and puts forward five measures of high-tech corporationmanagement from the point of hypercycle theory; it studies the relations between cariousinnovation of high-tech corporation innovation system and sets up models of innovation motiveforces and resistances, after that it analyses the alternation laws of innovation motive forces andresistances in each stage of high-tech corporation lifecycle, puts forward some measures tostrengthen motive forces and lessen resistances and then from the angle of dissipative structuretheory, it researches into relevant questions of high-tech corporation innovation management; itstudies the location, identification, cultivation, cultivation and protection of high-tech corporationcore competence and researches into the key elements of core competence management in eachstage of lifecycle and then from the angle of synergetic, it discusses several questions ofhigh-tech corporation core competence management; it studies seven common characteristics ofsuccessful high-tech corporation culture and puts forward the ways and measures to cultivate andinnovate high-tech corporation culture after that it researches into the characteristics ofcorporation culture in each stage of lifecycle and discusses relevant questions of high-techcorporation culture management from the point of hypercycle theory;' it studies interplay ofhigh-tech corporation system and environment and researches into the development andmanagement of high-tech corporation ecology system after that it analyses the construction anddisintegration of high4ech corporation strategic alliance.

剖析了高新技术企业系统的要素、结构、环境与功能，比较了高新技术企业系统与一般企业系统的异同；剖析了高新技术企业在生命周期各阶段的特点及衰退的管理根源，探讨了高新技术企业生命周期与一般企业生命周期、产业生命周期，产品生命周期和生物生命周期的异同；研究了高新技术企业生命周期各阶段的功能耦合，提出了实现高新技术企业系统内部耦合及系统与外部环境耦合的途径：以耗散结构理论的基本概念为基础，总结归纳出系统演化的六大内在规律，据此提出了高新技术企业生命周期管理的六个原则；提出了高新技术企业系统的四个序参量，剖析了生命周期各阶段序参量的协同与竞争，进而提出了高新技术企业管理的四大原则；研究了高新技术企业中的各种循环，从超循环理论的角度提出了高新技术企业管理的五大措施；研究了高新技术企业创新系统中各种创新的关系，建立了创新动力和创新阻力模型，剖析了生命周期各阶段创新动力与阻力的变化规律，提出了增强创新动力与减小创新阻力的措施，从耗散结构理论的角度研究了高新技术企业创新管理的相关问题；研究了高新技术企业核心竞争力的定位、识别、培养及保护，剖析了生命周期各阶段核心竞争力管理的要点，从协同理论的角度研究了高新技术企业的核心竞争力管理的几个问题；剖析了成功的高新技术企业文化的七个共同特点，提出了培养和创新高新技术企业文化的途径及措施，研究了生命周期各阶段企业文化的特征，从超循环理论的角度探讨了高新技术企业文化管理的相关问题；研究了高新技术企业系统与环境的交互作用，探讨了高新技术企业生态系统的开拓与管理，剖析了高新技术企业战略联盟的组建与解体。

Part II The experiment study on anti-tumour effect of dendritic cells derived from peripheral blood monocyte of patients with oral cancer after pulsed by tumour antigen in different ways. Collected 50ml peripheral blood from each patients and volunteers to induced DC in vitro. We parted the test group and control group into three subgroups respectively, untreated group A, freezed and thawn group B, RNA transfected group C. To group A, after induced DCs for five days , we added 200U/mlTNF-α in each hole and cultured on. To group B, we added protein antigen (DC/ tumor cells = 1 : 1 ,each hole) for 4h at 5d, and then added 200U/mlTNF-α in each hole and cultured on. To group C, transfected immature DC with tumor cell RNA(DC:RNA=1×105/ml: 5ug)at 5d, operated completely with the specification of invitrogen DMRIE-C reagents. After transfection we replaced transfection agent with complete medium, and then added 200U/mlTNF-α in each hole and cultured on.

第二部分口腔癌患者外周血来源的树突状细胞体外负载抗原后抑瘤作用的实验研究无菌采集20例口腔癌患者和20例健康志愿者外周血50ml，分离单个核细胞体外诱导培养DC，并将实验组和对照组细胞各分为三组，A组为未处理组，B组为冻融组，C组为RNA转染组。A组DC在培养5d后，每孔加入TNF-α200U/ml；B组DC培养第5d后，每孔中加入癌细胞蛋白抗原，使DC/肿瘤细胞=1：1，继续培养4h后，每孔加入TNF-α200U/ml；C组将培养至第5d的未成熟DC用肿瘤细胞RNA进行转染(DC：RNA=1×105/ml： 5ug，按照invitrogen DMRIE-C试剂说明书进行操作)，转染结束后用完全培基取代转染剂，每孔加入TNF-α200U/ml。

But we'd like to hear more from you on this. Then we shall see what can be done. Eng lishtang.com

但是我方愿意听取你方在这方面更多的建议，然后看看我们可以做些什么。

A champion of the music of his homeland, Belohlavek navigates Bohemia's great river the Moldau from its gentle source then on through the middle of Prague.

出生於捷克的贝劳拿域是演绎捷克音乐的名家，将为我们作向导，畅游波希米亚著名的莫尔道河，从恬静的源头一直走到布拉格的中部。

A champion of the music of his homeland, Belohlavek navigates Bohemia's great river the Moldau from its gentle source then on through the middle of Prague.

出生於捷克的貝勞拿域是演繹捷克音樂的名家，將為我們作嚮導，暢遊波希米亞著名的莫爾道河，從恬靜的源頭一直走到布拉格的中部。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。