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Study on the Crystallization Process of Labeled GL-7-ACA Acylase CA130 Complex 7β-bromoacetyl amino cephalosporanic acid (BA-7-ACA), an analog of glutaryl-7-amino cephalosporanic acid (GL-7-ACA), can inhibit and specifically alkylate GL-7-ACA acylase (CA130) from Pseudomonas sp. 130, forming a carbon-carbon bond between BA-7-ACA and the C-2 on indole ring of Trp-β4 residue of CA130.Here we reported that BA-7-ACA labeled CA130 (BA-C130) could self-catalyze the hydrolysis of BA-7-ACA during crystallization process. The hydrolysis was confirmed to be a reaction analogous to the one of GL-7-ACA by comparative MALDI-TOF (matrix-assisted laser desorption/ionization-time of flight) spectrometry analysis.

二、GL-7-AcA酰化酶CA130标记复合物的结晶过程研究溴乙酰氨基头孢烷酸（7β-bromoacetyl amino cephalosporanic acid，BA-7-ACA）作为戊二酰-7-氨基头孢烷酸（GL-7-ACA）的类似物，不仅能够抑制GL-7-ACA酰化酶CA130的活力，而且能通过在BA-7-ACA和CA130的β亚基第四位色氨酸吲哚环二位碳原子之间形成碳-碳共价键而将CA130特异的烷基化。

One people huddles up on bed , has no way to fall asleep, be thinking of the absurd problem in the brain, dark cools down just like a gigantic cobweb is good with my parcel apud , profound cotton-wadded quilt lining , my feeling, from fishing out mobile telephone under the pillow, but find that do not know should whom to fight to, thereupon, unwilling hold on to self knee lightly , cannot only be capable to do the cold bar then like one child holds self tightly one point in squeezing time of pillow in , in headman indent quilt,...

一个人蜷缩在床上，无法入睡，脑子里想着莫名其妙的问题，黑暗犹如一张巨大的蛛网把你包裹在内，厚厚的棉被里，我感觉好冷，从枕头下掏出手机，却发现我不知道该打给谁，于是，无奈的又塞回枕头中，把头缩进被子里，轻轻的抱住自己的膝盖，像一个孩子把自己抱紧一点，那么，就不会冷了吧

The writer, started from the reading of the early book " Force et signification" by Derrida, makes a minute exploration on the original context within which the deconstruction theory arises so as to demonstrate Derrida's own averment that deconstruction is not " a game on the chessboard without a bottom", i.e., a kind of absolute solution and negation, deconstruction is at the same time an unfreezing constitution.

作者从德里达的一部早期文本《力与意谓》的解读入手，细微地探寻了解构理论发生的初始语境，以证明德里达自己申辩，解构不是"无底棋盘上的游戏"，即一种绝对的消解和否定，解构同时是非凝固化的建构。

To give the definition of the mass concrete and to concrete\'s cracks together with practical meanings of control ,and give a brief review on the research in the field at home and abroad, at last, methods to control temperature crack of the mass concrete from several aspects such as design measures,concrets material, construction schedules.etc are summed up; To explain the physical mechanics of the concrete and computing methods-Difference Method and Finite Element Method, and illustrate the application of their specific methods repectively;Several temperature factors that can influence the temperature stress of mass concrete baseplate are analyzed. The influence degree of temperature factor can be got through the ANSYS simulation computation the control of temperature can control the temperature stress, consequently control the temperature crack of mass concert baseplate; through the influence of the crack resistance ability of mass concrete baseplate which takes the reinforcement as a part is analyzed. By ANSYS simulation, the best suited volume ratio of reinforcement is found out, at same time the layout of reinforcement with the same volume ratio can also affect the resistance ability of mass concrete baseplate. Sum up of above analysis, anti-crack function of steel bar in early mass concrete baseplate is obvious, so it\'s necessary to consider beneficial effect of construction steel bar in research of temperature control on mass concrete baseplate.

本文先容了大体积混凝土及混凝土裂缝的基本概念，对国内外在这个领域里的研究现状作了扼要叙述，并从结构设计、混凝土材质、施工措施等方面总结了大体积混凝土温度裂缝控制的方法及防止措施；阐述了混凝土的基本物理力学性能，总结了大体积混凝土温度计算的方法——差分法和有限元法，并分别举例说明其具体应用的方法；分析了影响大体积混凝土底板温度应力的几种温度因素，利用ANSYS模拟计算，得出了每种因素的影响程度，通过分析提出了在这几种温度影响因素下如何通过控制温度以达到控制温度应力从而来控制裂缝的具体建议；针对大体积混凝土基础底板中配筋的不同对混凝土底板抗裂能力的影响不同，利用ANSYS进行模拟分析，得出底板中最合适的体积配筋率，同时分析了在相同体积配筋率下钢筋的具体布置对大体积混凝土基础底板抗裂能力的影响，分析表明，配置钢筋对混凝土结构的开裂阻止作用明显，在研究大体积混凝土温度裂缝题目时，要考虑钢筋在其中的有利作用。

On the other hand what incensed him more inwardly was the blatant jokes of the cabman and so on who passed it all off as a jest, laughing 1530 immoderately, pretending to understand everything, the why and the wherefore, and in reality not knowing their own minds, it being a case for the two parties themselves unless it ensued that the legitimate husband happened to be a party to it owing to some anonymous letter from the usual boy Jones, who happened to come across them at the crucial moment in a loving position locked in one another's arms, drawing attention to their illicit proceedings and leading up to a domestic rumpus and the erring fair one begging forgiveness of her lord and master upon her knees and promising to sever the connection and not receive his visits any more if only the aggrieved husband would overlook the matter and let bygones be bygones with tears in her eyes though possibly with her tongue in her fair cheek at the same time as quite possibly there were several others.

253另一方面，他在内心深处更感到愤慨的是出租马车夫之流恬不知耻地开的玩笑。他们把整个事件当成笑料，肆无忌惮地放声大笑，装作对事情的来龙去脉了如指掌，其实他们心里糊里糊涂。这本来纯粹是两个当事人的问题，除非那位合法的丈夫收到密探的一封匿名信，说是就在那两人相互亲昵地紧紧搂抱着的关键时刻，给他撞上了，从而就促使那位丈夫去留意他们那暖昧关系，导致家庭骚乱。犯了过错的妇人跪下来向当家的告饶，只要这位受了损害的丈夫肯对此事抱宽恕态度，既往不咎，她就答应今后与那人断绝关系，再也不接受他的访问。她热泪盈眶，然而兴许长着一张标致脸蛋儿的她，同时还偷偷吐舌头呢，因为很可能还有旁的好几位哩。

It was suggest that the yield of tobacco in unit area was significant increasing and the quality was improved along with the altitude increase in Liuyang tobacco area. The tobacco leaves production at the place where the altitude from 400 meter and 900 meter was propitious to postpone the bearing period and growth and development of tobacco plants. It was the base of maturation endurance and could advance the tobacco leave maturation degree of all parts of tobacco plant. And it could hasten the orange tobacco leave ratio of the whole tobacco plant. It was boosted leaves unwrapping of top tobacco plant and the statuses of tobacco leaves, especially the top leaves, were significantly improved. The tissue structure of all parts leaves on tobacco plant were more loosen and the colour and luster of tobacco leaves was more vivid. The nicotinamide content in top leaves were digressive at a certain extent, on the contrary, the contents of the reductive sugar and kalium were increased at different extent. At the same time, it was benefit to play down the pesticide rudimentai of tobacco leaves and increased security of tobacco leaves.

结果表明：在浏阳烟区随着海拔高度的增加，单位面积烟叶的产量有着明显的增加，烟叶质量也有一定程度的改善；在海拔400—900m的地方进行烟叶生产，有利于延长烟株大田生育期，有效缓解浏阳烟区7、8月份的高温逼熟现象，有利于烟株的生长发育，为养好烟株的耐熟性打下良好的基础、促进各部位烟叶成熟度的提高；同时可以有效的促进烟株各部位橘黄烟叶比例的上升，对上部烟叶的开片也有着明显的促进作用，烟叶身份特别是上部烟叶的身份得到明显的改善，各部位烟叶组织结构更加疏松，烟叶色泽更加鲜亮；上部烟叶烟碱含量在一定程度上有所降低，各部位烟叶还原糖、钾含量有着不同程度的增加烟叶香气特征更加明显；香气量有一定程度的增加，吸位更加醇和、余味舒适；同时也有利于减少烟叶的农药残留量，提高烟叶的安全性。

People who practice Falun Gong will realize its harm to the society and will not take part in activities like that any more," Ji said,"On the other side, as time going on, when what Falun Gong has said is different from what it has done, people will have a clear look on its self-betrayal acts.

很多人都会认清楚自己的行为对社会的危害，不会参与这些东西。&纪烈武说，&另外一个方面呢，随着时间的推移，法轮功过去的说法和现在的表现越来越不一致的时候，人们对法轮功这种自身的暴露，自身的行为，人们也会看得越来越清楚。

In order to clean the ink, thorough and cleanly you.② all ink rolls product inks and ink traces, the best parts from the inking, close to the monlchamus tuhung area rollers on Isaac gasoline, so that waste ink wash the Union Jack from the rubber knife edge upper flow into the collecting ink Manger after a period of time boilouts, the roller clean.

在冲洗不朱时，为了彻底而肮脏不天纯不兴掉所有不朱辊上的积不朱和不朱色陈迹，最坏从输不朱部件上部，在挨近不朱斗区的不朱辊上浇撒汽油，这样，冲洗焉的宝不朱便从橡胶刀口上部流入收不朱槽边，经过一段工夫的冲洗，不离能把不朱辊洗肮脏。

Action research is adopted as the research method. Based on the difficulty faced while teaching music, this researcher feels that students in general lack the motivation to be creative. Eurhythmics, a favorite activity of students, is thus used as the main strategy to design teaching activities, with the aim to enhance the ability of improvisation. In addition, experimental teaching is adopted and the results presented by the researcher with a behavior observation record sheet and statistics, as well as audiovisual files of improvisation and musical diaries from the students. It is a combination of qualitative and quantitative research, whose results are presented as follows: 1. Designing the principle of a eurhythmics lesson: It starts from "listening" and applies elements of eurhythmics (force→space→time→relationship→locomotor →group formations) in teaching rhythm. It not only accumulates musical ability and experience, but also becomes the source of improvisation.

研究方法采行动研究法，根据研究者在教学现场所面临的困境：学生普遍缺乏创作的动机，因此，以提升即兴创作能力为目标，运用学生最喜爱的肢体律动为教学策略，设计教学活动行动方案，并且采用实验教学，以行为观察纪录表与统计、即兴创作影音档案及音乐日记作为呈现实验结果的依据，是属於质、量并陈的研究，其研究之结果分述如下：一、律动课程之设计原则：由「聆听」开始，运用律动的元素（力量→空间→时间→关系→移动→团体组成等）於节奏教学中，不但累积了音乐能力与经验，成为即兴的创作来源。

Bank sources told reporters that the key is in fact now unreasonable categories, personal loans purchase operations at least three risk protection : First, protect property suffered major accidents and natural disasters, the risk of collateral damage to housing property insurance; Second, lenders regular credit life insurance, mainly in the loan owing on the loan period of injury as a result of illness or accident and incapacity or death of bank guarantee safe recovery loans to the families of those who continue to live; Third mortgage purchase insurance performance bond, mainly because when the guarantor of death, unemployment causes an inability owing on the loan, insurance companies on behalf of their debt to banks liquidated I, at the same time exercise the right to recover from the collateral, or to the insured recover compensation awards; Now the market is the impact of policies, mostly property insurance, and lack of a man-made object of the life insurance products.

银行有关人士告诉记者，其实现在不合理的关键是险种问题，个人贷款购房业务至少需要三类风险保障：一是主要保障房产遭受意外事故和自然灾害毁损的风险的抵押住房的财产保险；二是贷款者的定期信用人寿保险，主要是在还贷期内贷款人因疾病或意外伤害而丧失工作能力或死亡的情况下，保证银行能安全收回贷款，贷款者的家人能继续居住；三是抵押贷款购房履约保证保险，主要是在当被担保人因为死亡、失业等约定原因无力还贷时，保险公司代其向银行清偿余债，同时行使追偿权，从抵押物中得到补偿。。。。。。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新