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The morphology and structure of reconstructed tissue was detected by microscope and scanning electron microscope.Results:(1) Compared with the control group, the cellular proportion of laminin group increased in 62 ~M phase, and decreased in Go~Gi phase significantly. As shown by the microscope, the cells of control group were in low density. The cells in mass connected tightly. The microfilament appeared reticular formation. The nucleus were the same in size. The cells of laminin group were in high density and put out so many lamellipodia, filopodia, which connected with the surrounding cells. The microfilament increased, elongated, and changed from reticulodromous to sarciniform, which reached to the pseudopods. The nucleus were different in size .(2) As shown by the inverted microscope and the cell growth curve, comparing with the controlgroup, cells of each test group increased evidently. The cellular proportion of each test group increased in S phase and G2 ~M phase, and decreased in Go~Gi phase significantly, but there was no considerable interations between LN and EGF;(3) As shown by the morphological observations, the cultured cat corneal endothelial cells formed an integrated membrane, and attached to the Descemets membrane closely, which was similar to the natural tissue. The cells connected tightly to each other, and some of them arranged in hexagon approximately.

结果：(1)层粘连蛋白组处于G_2～M期细胞比例较对照组显著提高，Go～G_1期细胞比例显著下降，提示层粘连蛋白促进内皮细胞DNA合成，及细胞分裂增殖；光镜下，对照组细胞分布成团状，细胞密度较低，细胞间连接紧密，细胞内微丝结成网状，细胞核大小一致；与对照组相比，层粘连蛋白组细胞生长旺盛，细胞密度高，向周边伸出大量板状及丝状伪足，细胞内微丝增多、拉长、集结成束，伸入伪足中，细胞核形状大小不一致；(2)倒置显微镜观察及细胞生长曲线显示，各组细胞数目随时间增加而明显增多，各实验组较对照组增生显著，EGF和LN联合应用组各时间点细胞数目最高；实验组处于S期和G_2～M期细胞数目增加，Go～G_1期细胞数目减少；提示EGF、LN单独及联合应用均可促进细胞增殖，但尚不能认为二者有交互作用；(3)倒置显微镜下，组织培养的猫角膜内皮细胞排列成密集的单层，细胞间连接紧密；组织学观察发现，培养的猫角膜内皮细胞形成完整的内皮层，贴附于脱水基质的后弹力膜上，与正常的角膜内皮组织结构相似；扫描电镜下，组织培养的猫角膜内皮细胞间紧密镶嵌排列，可见某些细胞呈近似六边形排列，细胞大小不甚一致，胞核清晰。

Right scene approaching out of control time, world alliance honor president the entire unification was finally anxious step rushed, waved the hand while makes an apology:"Is unfair to everybody extremely, as soon as I receive the emergency case to report on immediately picks the mine round trip from Saturn to catch up with, hurried in forgot to inform everybody unexpectedly, finally let everybody take the trouble has waited for!"

正当场面近乎失控之时，世界联盟荣誉主席全统一终于急步闯了进来，边挥手边致歉道：&非常对不起大家，我一接到紧急情况报告就马上从土星采矿场往回赶，匆忙之中竟忘了通知大家，结果让大家费心等待了！&

Methods In the study,experimental animals were divided into three groups. Thiamazole was used to replicate the model of maternal pregnant hypothyroidism. The infant rats were innate hypothyroidism. T4 substitution group rats were administrated with T4 from postnatal 0 day as well as the same days normal neonatal rats as control. TdT-mediated dUTP nick end labeling was carried out to detect the positive cells of PCD in cortex and hippocampus of postnatal 7,14 and 21 days rats. Immunohistochemical staining technique was employed to detect Bcl-2 and Bax proteins in relevant sections. Experimental data were statistically analysed by One-Way ANOVA, SNK-q test and Spearman rank correlation analysis.

本研究实验动物分为三组，采用甲巯咪唑复制甲状腺功能减退孕鼠模型，出生后仔鼠为先天甲减鼠，T4替代组于仔鼠出生后即日起腹腔注射T4，剂量为20μg∕，同时设一正常对照组；应用原位末端标记方法检测出生后7、14、21d仔鼠大脑皮层、海马PCD阳性细胞，并应用免疫组化SP法检测相应部位Bcl-2及Bax蛋白；用单因素方差分析（One-Way ANOVA）、SNK-q检验及Spearman等级相关分析进行统计处理。