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Based on the microecological principle, lactic acid bacteria were isolated from the intestinal tract of healthy wood frog by two kinds of isolation methods and purified and identified further.

用2种方法从健康林蛙肠道菌群中分离乳酸菌,并进一步纯化及鉴定。

The result: the Heilongjiang wood frog\'s survival ratios were 100%at 14℃and 1℃after 72h low temperature stress and survival ratio fell to 84.6%after 24h exposed at -2℃.

在此基础上测定1℃组及室温组G6PDH及EST同功酶活性及肝脏蛋白变化。

Lactic dehydrogenase isoenzyme of deferent tissue and organ of china wood frog in changbai moumtain were studied by PGE,the result show that the most of enzyme bands are twelve,the less are seven.

用聚丙烯酰氨凝胶电泳对长白山中国林蛙的不同组织器官的乳酸脱氢酶的同工酶进行了分析,结果显示出最少为 7条酶带,最多为 12条酶带。

Major raw materials : Chinese wood frog s oviduct , corn salad oil , palm oil

中国林蛙油,玉米色拉油,棕榈油

The content ofone liver protein band (R_f=0.79) at 1℃was significantly higher than at room temperature (P<0.05).On the 2-D native-PAGE/SDS-PAGE gel, the content of P spot (MW=51kDa, R_1=0.47on the 1D native PAGE gel) at 1℃was significantly higher than at room temperature (P<0.05), and the content of N spot (MW=65 kDa, R_f=0,94 on the 1D native PAGE gel) at roomtemperature was significantly higher than at 1℃. These protein spots may be related with the adaptation to the low temperature of the Heilongjiang wood frog.

黑龙江林蛙肝脏蛋白电泳显示,胶浓度6%的肝脏PAGE图谱分辨率高、谱带多,可分辨出15~19条谱带,其中1℃组R_f=0.79蛋白含量比室温组显著增加(P<0.05)。2-D native-PAGE/SDS-PAGE电泳时,胶浓度为12%时分离效果较好,蛋白斑点较多。1℃组中P蛋白斑点(MW=51kDa,一向PAGE的R_f=0.47)显著性上调(P<0.05),N蛋白斑点(MW=65kDa,一向PAGE的R_f=0.94)显著性下调(P<0.05),推测这两种蛋白可能与黑龙江林蛙在低温环境下的适应性变化有关。

It is significance to further study the tolerance mechanism ofHeilongjiang wood frog during hibernation. Its cold-tolerant mechanism has an importantapplied value and a great potential for exploitation and utilization.We choosed Heilongjiang wood frogs from Yichun, Heilongjiang province.

本试验选取黑龙江省伊春地区黑龙江林蛙为研究材料,观察黑龙江林蛙在环境温度为室温(14℃)、1℃和-2℃条件下的存活率,并采用2-D native-PAGE/SDS-PAGE电泳、酶活性比色法、同工酶电泳等生化技术检测不同温度环境下肝脏蛋白质及代谢酶的活性变化。

Said simple building comprises building body, roof and pool inside room; the lower building body is complete closed, the upper building body is provided with pillar on lower building body, and is circled with sun-shading net around pillar, the outside of sun-shading net is equipped with movable curtain; the roof is straw roof and has two sides along ridge; the pool inside the room comprises cultivation pool and laying-up basin, each cultivation pool is provide with a overflow bench, the bench surface is equipped with a cushion and the cushion is fixed on slope bench with a plurality of bracing column of same height, and a bamboo curtain is placed on the bracing column for young wood frog rest.

该简易房,包括房体、屋顶和室内池,其下房体为全封闭体;上房体是在下房体上设有房柱,再用遮阳网沿房柱排列的走向围拢,在遮阳网的外面还设有活动帘;屋顶为稻草屋面,沿屋脊坡向两面;室内池包括养殖池和蓄水池,每一个养殖池中均设有漫坡台。坡台面上设有软垫,并有相同高度的多根支撑柱钉穿过软垫固定在坡台上,支撑柱钉上再放置供幼蛙栖身的竹帘。

Jot down, the wood's frog oil has to reinforce body fluid to keep looks , defering decrepitude, restoring virility benefit spirit , the smooth lung keeps yin etc.

公司生产的林蛙珍油被评为吉林省消费者协会授予"信誉产品"称号,2005年吉林省名牌农产品,2006年被评为吉林市名牌产品。

Frog :"Hello , uncle Woodpecker , I have a question to ask you , what is happiest ?"

青蛙:"啄木鸟叔叔,您好!我有一个问题想问您:做什么事情最快乐呢?"

I found a wrinkled frog on the ground in my garden.

我看到在我家院子的地面土蛙了。

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推荐网络例句

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。