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The invention discloses a preparing method of bacillus alcaligenes strain Alcaligenes sp., CCTCC No.M206121 of degraded o-nitrophenol, which comprises the following steps: allocating inorganic salt culture medium with dibasic sodium phosphate, monobasic potassium phosphate, manganese sulfate, copperas, addex-magnesium, calcii chloridum, blue copperas, white copperas and hydrogen dioxide solution; adjusting pH value; sterilizing under high temperature; enriching bacterial; sampling from sludge; allocating suspending liquid with soil sample and inorganic salt; seeding into ortho-nitrophenol fresh organic salt culture medium with finite volume seeding quantity; keeping the temperature; culturing on shaker; getting enriched culture; separating and purifying enriched culture in LB solid culture medium.

本发明公开了一种降解邻硝基酚的产碱杆菌菌株及制备方法，产碱杆菌为Alcaligenes sp。，CCTCC No.M206121。其制备步骤是：首先是配制无机盐培养基，成分为磷酸氢二钠、磷酸二氢钾、硫酸锰、硫酸亚铁、硫酸镁、氯化钙、硫酸铜、硫酸锌和双蒸水，调pH值高压灭菌；其次是菌种的富集，从污泥中取样，将土样与无机盐培养基配制成悬浮液，将悬浮液以一定体积接种量接种到含邻硝基酚的新鲜无机盐培养基中，恒温，摇床培养，获富集培养物；第三是将富集培养物在LB固体培养基中分离和纯化。

The paradise is beautiful ,fresh, unreal, grand pretty and coquettish , and full of the danger.

人生其实就是一程冒险的旅途美丽，新鲜，虚幻，壮观，妖艳又充满危险。

Then she filled it with the fresh ,cool water, corked it tightly, and started with it to the hayfield.

她接着往里灌满了新鲜清凉的井水，紧紧地塞上木塞，提着水就往干草地里去了。

The results showed:(1) the propagation coefficient were from 0.8 to 6.8 in different development phase, the highest propagation coefficient appeared in June, July in dormancy stage and September in initial stage of vigorous growth;(2) all of biological characters, such as cormel's fresh weight, size, height, root length, roughness and root quantities, presented 1% significant difference.

结果表明：(1)不同生长发育期无性繁殖系数在0.80~6.80之间浮动，休眠期的6、7月和旺盛生长初期的9月繁殖系数较高；(2)不同生长发育期无性繁殖的子球鲜质量、最大直径、子球高度、根系长度、根基粗度及根系数量等生物学性状均呈现1%水平极显著差异。

Although uncle failed to fulfill his plan due to economic reasons, every time he talks about this event, the regret in his face and the eagerness for Cornel University in his eyes often are still fresh in my memory!

尽管由于经济的原因，最终叔叔没有实现他的计划，但每次谈起这件事来，他脸上的遗憾和眼里对康奈尔的向往神情令我记忆犹新！

Although uncle failed to fulfill his plan dueto economic reasons, every time he talks about this event, the regret in his face and the eagerness for Cornel University in his eyes often are still fresh in my memory!

尽管由于经济的原因，最终叔叔没有实现他的计划，但每次谈起这件事来，他脸上的遗憾和眼里对康奈尔的向往神情令我记忆犹新！

Note: if there is not after use, please stop using or consulting.06 xi bai water emulsion: mild texture, brushed facial skin fresh moist, corneous, can better absorption of white skin clean, ensure whitening essence is very important in nursing.

注意事项：用后如有不适，请暂停使用或咨询。06皙白补水乳液：极致温和的质地，拂去面部角质，肌肤清新润泽，可以更好的吸收后续的净白亮肤精华素，确保美白护理中至关重要的环节。

Xi bai water emulsion: mild texture, brushed facial skin fresh moist, corneous, can better absorption of white skin clean, ensure whitening essence is very important in nursing.

06皙白补水乳液：极致温和的质地，拂去面部角质，肌肤清新润泽，可以更好的吸收后续的净白亮肤精华素，确保美白护理中至关重要的环节。

The Corps de Ballet of China presented " Red Lantern" on the 100th Anniversary Hall of Peking University, retelling this ancient Chinese tale through a fresh art form.

中央芭蕾舞团在大讲堂上演的芭蕾舞剧"大红灯笼高高挂"，用一种崭新的艺术形式重新阐释了这个古老的中国故事。

The cytoplasmic trehalose concentration in red blood cells, free hemoglobin and ATP level were determined at different incubation temperatures (4, 22 and 37℃), different trehaolse concentrations (0, 200, 400, 600, 800 and 1000 mmol/L ) and different incubation times (2, 4, 6, 8 and 10 hours), the cytoplasmic trehalose, free hemoglobin, hemoglobin and mean corpuscular volume in fresh RBCs and RBCs strored for 72 hours at 4℃ were compared, when loading condition was ensured.

在不同孵育温度（4、22和37℃）、孵育时间（0、2、4、6、8、10小时）、不同负载缓冲液浓度（0、200、400、600、800、1 000 mmol/L）条件下检测新鲜红细胞对海藻糖的成功负载量及红细胞各项理化指标；在固定负载条件下，对新鲜红细胞和4℃保存72小时红细胞海藻糖负载、游离血红蛋白、血红蛋白和红细胞平均体积进行了比较。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。