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Acellular spinal cord ( freeze thawing + 3% sodiumdeoxycholate + DNaseⅠ,RNaseA)and fresh spinal cord of rats were implanted into paravertebral muscles of rats.

评价脱细胞脊髓支架的免疫原性,为其在组织工程中的应用奠定理论基础。

A little of the diaylsed solution was dilluted with distilled water (1:4) and applied to metallic sample plate following negative staining with sodium phosphotungstate solution. The sample was freeze-dried and examined by transmission electron microscopy 1.4 IFvs size and size distribution measured 1.5 The shape morphology of IFvs 1.6 Measureing the encapsulation efficiency and loading capacity of Liposomal Gd-DTPA A sample volume of 5ml was sent to analyze (Zhongshan University Analytical Center, Guangzhou).

1.6 Gd-DTPA脂质体包封率及载药量的测定取5ml前述离心后所得上清液送交中山大学分析测试中心,采用高频电感耦合等离子发射光谱法测定离心液中Gd-DTPA含量,用加入总的Gd-DTPA量减去离心液中的Gd-DTPA含量即得包封量。

The first, selecting Mannitol of polyhydric and Dextran ofpolysaccharide as subsidiary material, In accordance with differentusing quantities, making up eight recipes, freeze-drying test is madewith eight recipes differently. By adjusting recipe, to select themethod of two factors three levels. The best recipe determined is thatof Mannitol(160mg/bottle) and Dextran(40mg/bottle), Solving theproblem of description、moisture(≤1.5%)、solution(≤6s)、stability(95.0~110.0%) etc., The quality of preparation has beenimproved.

首先,选择赋形剂多元醇类的甘露醇和多糖类的右旋糖酐作为辅料,按照不同的用量,组合成8种处方进行冷冻干燥试生产,采用二因素三水平的满因子分析法,通过处方调整,确定甘露醇160mg/瓶、右旋糖酐40mg/瓶为最佳处方,解决了冻干制剂在性状、水分(≤1.5%)、溶解性(≤6s)和含量稳定性(95.0~110.0%)等方面的不足,提高了产品质量。

Expression rate induced by thrombin was 50.88% and PAC?1 re?expression was 54.55%. It is concluded that the ability of recovered lyophilized platelets added with reversible activation?inhibitors, DMSO and trehalose to aggregate and coagulate has showed no significant difference as compared with FPRP. The reversible activation?inhibitors can decrease CD62p expression of lyophilized platelets, and may enhance their survival ability and prolongate survival time. Therefore the efficiency of lyophilizing platelets can be improved based on this freeze?drying procedure.

添加血小板可逆性激活抑制剂,海藻糖和DMSO后的冻干血小板,其聚集活性和促凝血功能与新鲜血小板无明显差异,血小板可逆性激活抑制剂降低了冻干血小板的CD62p表达,增强了冻干血小板的生存能力,因而延长了其生存时间,因此可以该冻干方法为基础进一步提高冻干保存血小板的效率。

In this paper, making the vacuum freeze-dry theory,the composition of equipment system, the craft as basis, the research of drying the pteridium aquilinum are actualized.

本文以真空冷冻干燥理论及其操作系统、工艺为基础,对蕨菜进行真空冷冻干燥工艺的试验研究。

The influence of drying parameters of freeze-drying on quality of pteridium aquilinum drying was investigated, and the impact of drying board temperature, drying chamber pressure and material thickness on Vc content was studied by nonlinear regressional orthogonal design.

考察干燥因子对真空冷冻干燥蕨菜品质的影响,用二次正交旋转组合设计的试验方法,分析搁板加热温度、干燥室压力和物料层厚度对蕨菜Vc含量的影响规律,建立了冻干因子与Vc含量的回归模型。

The optimal combination of the preserved quality parameters for the freeze-drying technology of pteridium aquilinum was obtained through optimized theory in nonlinear method.

并利用非线性优化理论确定了搁板加热温度为42.5℃、干燥室压力为55Pa、物料厚度为16.3mm时,蕨菜的Vc质量比可达2.387mg/g。

The quality indexes such as color and vitamin C of the dried Pteridium Aquilinum with the MVD method were quite similar to those with freeze drying method, which had a remarkable advantage over those with hot air drying method.

在蕨菜的微波真空干燥过程中,微波功率对干燥速度的影响要高于真空度,并且提出了蕨菜含水率与微波功率、干燥时间和真空度之间的回归模型;对3种方法干燥后的蕨菜在颜色、维生素C含量和复水性方面进行对比,结果表明:微波真空干燥的蕨菜的复水性优于热风干燥和冷冻干燥;微波真空干燥后的蕨菜品质与冷冻干燥几乎相同,明显高于传统的热风干燥品质。

Based on the freeze-drying technology of the pteridium aquilinum, the color protection, brittleness keeping, rehydration and Vitamin C surplus rate of dried pteridium aquilinum were discussed in particular.

研究侧重从干制蕨菜的护绿、保脆、复水性及Vc的保存率等方面,讨论了蕨菜的冷冻干燥生产工艺,并得出了蕨菜冷冻干燥的最佳工艺条件,可为技术工业化提供理论依据。

Keywords Pyrology;freeze-drying;human bone mesenchymal stem cells;lyoprotectant

热工学;冷冻干燥;人体骨髓基质干细胞;冻干保护剂

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