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METHODS: Olfactory bulb was sterilely collected from rats, digested, and centrifuged. After removal of the supernatant, the olfactory bulb was resuspended with DMEM/F-12 medium containing 0.2 volume fraction of fetal bovine serum. The diluted monoplast suspension was equally assigned into 3 samples, and separately used for simple modified differential adherence, simple serum-free conditioned medium, and modified differential adherence + serum-free conditioned medium.

①无菌条件取大鼠嗅球组织，消化、离心去除上清液后，用含体积分数为0.2胎牛血清的DMEM/F-12培养基重悬，稀释的单细胞混悬液均分成3份，分别用于单纯改良差速贴壁、单纯无血清条件培养液纯化和改良差速贴壁+无血清条件培养液纯化的实验。

"A lot of a consumers go into the shopping process thinking 'the first thing I need to find is a way to get this shipped to me for free.'" Many shoppers "are willing to make a compromise on the price of the product in return for free shipping," says Mudd.

&很多消费者进入购物过程思维'的第一件事，我需要找到一个办法让这发运给我自由'&，许多购物者&，都愿意作出妥协，对产品价格在换取免费送货，说：& mudd 。

Male flowers: sepals mostly 5, imbricate to valvate, free; petals 5, smaller or nearly equal to sepals; disk glandular, usually 5, and opposite sepals; stamens 10-20; filaments free, inflexed in bud; anthers muticous; pistillode absent.

雄花： 多数的萼片5，覆瓦状到镊合状，离生；花瓣5，小或近等长于萼片；花盘具腺，通常5，与萼片对生；雄蕊10-20；花丝离生，在芽中内折；花药钝；无退化雌蕊。

From 1982 to 1988, 14 cases (8 females and 6 males) with granular myringitis had been operated under local anesthesia, and used an operation microscope to remove all the granulation tissue which existed over the ear drums. After we had removed the granulation tissue over the ear durms, we had performed free ear canal skin autograft for 8 cases – group A, and we had performed temporalis fascia with free ear canal skin autograft for 4 cases (small perforation was made by accident during the surgery due to large granulated area,﹥50%)– group B, but we had covered silastic sheet over the denuded drum surface for the other 2 cases –group C.

14例肉芽性鼓膜炎为自民国71年至77年间的住院手术病人，皆在耳局部麻醉后，於显微镜下刮除鼓膜上所有肉芽组织，其中8例於刮除鼓膜上所有肉芽组织后有移植外耳道的皮肤－A组，另外4例则因鼓膜上肉芽组织范围大於50%以上，致手术中意外地造成鼓膜小穿孔，需立刻加铺颞肌膜后，再移植外耳道的皮肤－B组，其余2例则只在刮除鼓膜上的肉芽组织后，直接铺上一块矽胶片－C组。

Human embryonic stem cells can differentiate into neuroepithelial progenitor cells in both feeder containing and feeder free systems,yet feeder free system has a relatively lower inductive efficiency with part of undifferentiated embryonic stem cells.

人胚胎干细胞在有饲养层和无饲养层体系中均可诱导分化形成神经上皮祖细胞，但无饲养层体系的效率相对较低，且有未分化胚胎干细胞残余。

This creates neurotically hungry "Grace-chase" expectations in the minds of naive seekers looking to get " high " on euphoria and altered states of consciousness rather than become truly clear and free , free of egoic attachments, aversions and delusions .

他们在那些寻求强烈的极度喜悦和转化的觉知的求道者们的头脑中创造了一种极为焦虑的、饥渴的&追逐恩典&的期望，而不是真正的清晰和自由，脱离自我的依附、厌恶和幻象。

CD44~+CD24~ cells in MCF-7 increased after cultured in serum-free medium. The cancer stem-like cells in MCF-7 grew as clonal nonadherent spherical clusters. The percentage of CD44~+CD24~ cells reached up to 29.23% after cultured 3 weeks in serum-free medium.

结论SP检测与CD44~+CD24~细胞检测是目前乳腺癌干细胞检测的两种常用方法，但两者用于同一种乳腺癌细胞系中的检测结果并不一致，均有一定的局限性。

UniLustre papers and boards and UltraLustre plastics are nonlaminated, film-free and foil-free, and made without solvents.

UniLustre文件和塑料板和UltraLustre是nonlaminated，电影和无箔免费的，没有溶剂的。

Unifoil's award-winning recyclable UniLustre papers and boards and its new recyclable UltraLustre? plastics, both of which are nonlaminated, film-free, foil-free and made without solvents, satisfy consumer-product-goods companies' sustainability initiatives.

Unifoil获奖的可回收UniLustre 文件和议会和新的可回收UltraLustre 塑料，这两者都是nonlaminated，电影免费，箔，不需溶剂进展，满足消费者的产品，商品公司的可持续发展倡议。

The results were as follows the change of vitamin C content was a "trial apex"-shape curve, higher in young fruit,incrustation and maturation stages, lower in other periods; the total acid content was the highest in young fruit, after that, it decreased rapidly, maintained lower level, and reached lowest when the fruit matured; the total content of soluble sugar inosculated with the fruit development, it was high when the fruit development was quick, and low when the fruit development was slow; before 59 d after anthesis, the reducing sugar content was high in the total content of soluble sugar and after that ,the nonreducing sugar content was high; before 45 d after anthesis, free amino acid content was obviously correlated with protein content, and they inosculated with the fruit development;4559 d after anthesis, free amino acid content increased quickly, and protein content decreased gradually, this was also the most shaping period of the embryo and endosperm.

结果表明，Vc含量呈&三峰&型曲线，幼果时、硬核后和成熟时较高，其他时期较低；有机酸含量以幼果期最高，以后迅速下降并维持较低水平，果实成熟时含量最低；可溶性总糖含量与果实发育状况基本相吻合，果实发育速度快时含量较高，慢时较低；在谢花后59 d以前，可溶性糖以还原糖为主，之后以非还原糖为主；游离氨基酸和蛋白质含量，在谢花后45 d以前两者呈高度相关，且与果实生长速度相吻合，谢花后4559 d是胚和胚乳的主要形成期，此期游离氨基酸含量迅速增加，蛋白质含量持续下降。西北林学院学报21卷第5期张传来等金光杏梅果实发育期间主要营养成分动态变化研究

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！