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On the basis of the kinetic equation of substrate reaction in the presence of urea or guanidine hydrochloride, all microscopic kinetic constants for the free enzyme and enzyme-substrate binary and ternary complexes have been determined. The results of the present studies indicate that:①In the presence of urea or guanidine hydrochloride, enzyme-substrate complexes lose their activity less rapidly than the free enzyme. Therefore, both substrates, NADPH and 7, 8-dihydrofolate, protect dihydrofolate reductase against inactivation.②The denaturation of dihydrofolate reductase by urea follows single-phase kinetics, and changes in enzyme activity and tertiary structure proceed simultaneously in the unfolding process, so it may be an"all or none"process.③The GdnHCl-induced unfolding of the dihydrofolate shows a biphasic transition, while the change in the enzyme activity is a single exponential process. The rate constant of inactivation is consistent with that of the fast conformational change. Therefore, the kinetic intermediate of protein unfolding should be a partially folded and inactive form.

我们根据在脲或盐酸胍存在下的底物反应动力学方程求得游离酶和酶底物二元、三元复合物的微观动力学常数，结果表明：①酶-底物二元、三元复合物的失活速度明显慢于游离酶，说明两个底物二氢叶酸和NADPH对酶的失活都具有一定程度的保护作用；②在脲作用下，酶的失活和构象变化均为单指数项过程，而且酶的活力丧失和三级结构变化是同时发生的，说明二氢叶酸还原酶的脲变性可能是一个"全或无"的两态过程；③在盐酸胍作用下，酶的构象变化为两相过程，而失活则是单指数项过程，酶分子构象变化的快相速度常数与失活速度常数基本一致，因此我们认为二氢叶酸还原酶的盐酸胍变性过程中存在一个没有活力、但仍具备一定空间结构的变性中间体。

Feel free to call me. If I'm free, I'll go with you.

如果我有时间，我就会跟你去。

Kathy Fillmore - Find free articles by expert author Kathy Fillmore for republishing on your websites, blogs and free offline magazines.

凯茜菲尔莫尔-找到免费文章由专家撰文凯茜菲尔莫尔为重新在您的网站上，博客和免费离线杂志。

Free at last free to lie powerless in the dirt, to be blown helplessly along the ground, and to lodge lifeless against the first obstruction.

终于自由使他自由到无力地躺在尘土中，无助的让风沿着地面将其吹走，碰到第一个障碍物便毫无生命的滞留在那里了。

Free at last, free to lie powerless in the dirt, to be blown helplessly along the ground, and to lodge lifeless against the first obstruction.

自由来自于对无情的微风的抵抗的幸运，它总是在最后获得自由，自由得面对一切，在地面之上是没有帮助，并且对抗着第一次的障碍。

Free at last free to lie powerless in the dirt, to be blown helplessly along the ground, and to lodge lifelessagainst the first obstruction.

终于自由使它自由到无力地躺在尘土中，无助地任风沿着地面将其吹走，碰到第一个障碍物便毫无生命地滞留在那里了。

Free at last free to lie powerless in the dirt, to be blown helplessly along the ground, to lodge lifeless against the first obstruction.

终于自由使它自由到无力地躺在尘土中，无助地任风沿着地面将其吹走，碰到第一个障碍物便毫无生命地滞留在那里了。

Free a last , free to lie powerless in the dirt, to be blown helplessly along the ground, and to lodge lifeless against the first obstruction.

终于自由，自由无力地躺在尘土中，无助地任风吹得满地跑，碰到第一个障碍无便毫无生命地滞留在那里了。

"Free at last" free to lie powerless in the dirt, to be blown helplessly along the ground, and to lodge lifeless against the first obstruction.

它"终于自由了"，自由到无力地躺在泥土上，沿着地面无力地被风吹走，一旦遇到障碍便死气沉沉地滞留在那里了。

Free at last free to lie powerlessin the dirt, to be blown helplessly along the ground, and to lodge lifeless against the first obstruction.

终于自由使它自由到无力地躺在尘土中，无助地任风沿着地面将其吹走，碰到第一个障碍物便毫无生命地滞留在那里了。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。