查询词典 frap

Aftcr the injury of the sciatic nerve,FRAP would be decreased in the correspondingpart of the spinal cord and gave a sharp contrastto that of the control rats and the uni...

认为在神经科学实验中可利用本法帮助准确定位受损神经的相应脊髓中枢，还可据此推断神经损伤模型的可靠程度。

The R^2 values of TEAC and FRAP also showed a higher correlation (water extracts, R^2=0.984; ethanol extracts, R^2=0.971). In HPLC analysis, the chromatograms of the water and ethanol extracts of HN with the highest antioxidant activity were established.

在HPLC的分析中，由於乞食碗具有最高的抗氧化活性，其水萃取物及乙醇萃取物已建立指纹图谱，卢丁发现可能是乞食碗萃取物中的一个重要活性成分。

The present study explored the effects of morphine on plasticity of spinal cord in three parts as follows: 1. the effects of morphine on dorsal root terminal in spinal lamina Ⅱ of spared root rat---- a quantitative study by FRAP histochemistry; 2. the effects of morpnine on synaptic reinnervation in spinal lamine Ⅱ of spared root rat----an EM quantitative study and 3. the effects of morphine on collateral sprouting of nerve fiber in spared root of rat----an EM quantitative study

本课题从三个方面探讨了吗啡对脊髓可塑性的影响：1、吗啡对备用根大鼠脊髓Ⅱ板层背根终末的影响——抗氟化物酸性磷酸酶组化定量研究；2、吗啡对备用根大鼠脊髓Ⅱ板层突触重建的影响——电镜定量研究；3、吗啡对大鼠备用根神经纤维侧支出芽的影响——电镜定量研究。

Mobility of membrane phospholipid and receptors: membrane phospholipid was labeled with the NBD-C〓-HPC and the mobility was measured with FRAP technique, the results showed that the membrane phospholipid mobility gradually deceased with the time of ligand stimulation.

1膜磷脂和受体流动性：利用NBD-C〓-HPC荧光标记细胞膜磷脂和荧光漂白后恢复技术测量膜磷脂流动性，结果显示膜磷脂流动性随配体刺激时间增加而逐渐下降。

The quantitative result of EM showed also that the numbers of unmyelinated fibers in spared root of morphine spared root and spared root rats were respectively 60% and 42% higher than that of unoperated side.

提示参与Ⅱ板层突触重建的部分突触前成分是来自备用根内无髓纤维的侧支出芽。6、切除一侧腰段大部分背根节后，对侧Ⅱ板层外侧份FRAP活性降低或消失，即鱼钩尖消失。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。