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In bioconv ertion experiment for spiramycin of No.5 transformant, the bioconversion product was analysed with TLC, bioautography, HPLC and mass spectrum.Results showed that the bioconvert product is propionyspiramycin. Propionyl acylase gene was l ocolized on 0.53 kb insert DNA fragment of recombinant pasmid pLJM95. Analysis result of DNA sequence showed that content of G+C is 68.2% for 0.53kb insert DNA fragment .

以No.5转化子对螺旋霉素进行生物转实验，其转化产物经薄层层析，生物显迹，高压液相色谱及质谱分析表明，与标准丙酰螺旋霉素一致，这说明 No.5 转化子具有将螺旋霉素生物转化为丙酰螺旋霉素的能力，丙酰化酶基因位于重组质粒，pIJM95 0.53kb 插入DNA片段上。

In order to get purified purified commercially luciferase, we selected whole gene fragment of Photinus pyralis as well as gene fragment deleting 18-nucleotide at 5'termination, which was synthesized according to P〓 template.

为了能得到商品纯的虫荧光素酶，将取自P〓质粒中的全长的北美萤火虫荧光素酶基因片断，及以P〓为模板合成的5＇端缺失18个核苷酸的虫荧光素酶基因片断。

HSP90 cDNA fragment cloned from chewing cane(Saccharum officinarum L.) was used as probe to produce a 2 097 bp cDNA fragment by using in silico cloning and splicing.

以克隆到的果蔗 Hsp 90基因片段作为探针，利用电子克隆和序列拼接方法获得一个全长为2 097 bp的cDNA序列。

We had preliminary researched on the mechanism of Stylosanthes guianensis cv. Mineirao resistant to anthracnose, a specific fragment of SG2950-1 was acquired from genome DNA of Stylosanthes guianensis cv. Mineirao by the technique of AFLP in our laboratory, we acquired both ends of the fragment of SG2950-1 by RACE from Stylosanthes guianensis cv.

我们已经对Mineirao柱花草的抗病机理进行了初步研究，并用AFLP分子标记技术从其基因组DNA中获得了一特异片断SG2950-1，利用快速扩增cDNA末端技术将基因组DNA特异片段的两端从Mineirao柱花草扩增出来，经测序后拼接得到MSRA基因的全长序列。

A series PCR amplification for differential control strains and DNA samples diluted gradient (1:10) have been used to evaluate the specificity and sensitivity of PCR assay established.Results 1. Detection of GAS by PCR assay: The 345bp specific fragment of speB gene were amplified in all the tested GAS strains including three strains of scarlet fever, whereas it was detected in none of the differential control strains. The lowest limit of detection was 6.5pg genome DNA of GAS strain. 2. Detection of corynebacterium diphtheria by PCR assay: The318bp specific fragment of toxB gene were amplified in all the tested toxigenic corynebacterium diphtheria strains, whereas it was detected in none of the differential control strains. The lowest limit of detection is 850fg/μl genome DNA of corynebacterium diphtheria strain. 3. Detection of Lp by PCR assay: The 340bp specific fragments of mip gene were amplified in all the tested Lp strains, whereas it was detected in none of the differential control strains including three strains of non-pneumophila.

结果：1、用PCR方法检测A组链球菌：以A组链球菌致热性外毒素基因speB为靶序列，设计的扩增引物对全部对照菌株的扩增结果为阴性，而全部A组链球菌参考株均能扩增出特异的345bp片段，其中包括三株猩红热链球菌，检测敏感性为6.5pg／μl DNA.2、用PCR方法检测白喉杆菌：以白喉外毒素基因toxB为靶序列，设计的扩增引物对全部白喉杆菌参考株均能扩增出特异的318bp片段，而全部对照株的扩增结果为阴性，检测敏感性为850fg／μl DNA.3、用PCR方法检测嗜肺军团菌：以嗜肺军团菌巨噬细胞感染增强子基因mip为靶基因，设计的引物对嗜肺军团菌14个血清型参考株均扩增出特异的340bp片段，而鉴别对照株包括三株非嗜肺军团菌均未扩增出任何片段。

The results showed that, Yak TGF-β2 gene fragment length of 559 bp, with ordinary cattle, human, pail troglodytes, mice corresponding fragment derived 98%, 95%, 94%, 93% respectively.

结果表明，牦牛TGF-β2基因片段长为559bp，与普通牛、人、黑猩猩、小鼠的相应片段的同源性分别达98%、95%、94%、93%。

And then, the pBI101.2 wirelike vector and the promoter fragment of 886bp were acquired. Becase the promoter was modified by the HindⅢand BamH I, the acquired target genes fragment, digested by the restriction endonuclease, was matched to the wirelike vector.

由于在启动子两端有人工设计合成的HindⅢ和BamHⅠ酶切位点，经上述双酶切消化后，回收目的基因片段与线状载体的粘性接头恰好匹配。

Made up of fragment s of preexisting rock; fragment al.

碎屑的由原来的岩石的碎屑构成的；碎屑的

A fragment audit model based on role was proposed to solve the problem that the network intrusion carried out by the policy of fragment reassembles according to the character is different in different OS leads to the decrease of the discernment of security audit systems.

提出了一种基于角色的分片审计模型，以解决因利用各个操作系统分片重组策略不同的特点进行网络渗透而导致安全审计系统识别能力降低的问题。

Physical models of fragment discharge process are established and the fragment discharge law is discussed by the rational simplification of parallel- cut blasting process in this paper.

通过对直眼掏槽爆破过程的合理简化，建立了排碴过程的物理模型，研究了排碴规律，并通过实例对其合理性进行了验证。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。