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Another pair of primers including the start codern and the terminer coden were designed to amplify the PAL gene. Two restriction enzyme XbaI and XmaI were added to the two primers respectively, which enable to insert this fragment to the prokaryotic expression vetor pCAMBIA2301-WCS.

在PAL 基因的编码框以启始密码子ATG 和终止密码子TAG 为两端设计一对特异引物，分别在引物5' 端引人XbaI 和XmaI 酶切位点，用来扩增PAL 基因并与pCAMBIA2301-WCS 构建表达载体pCAMBIA2301-PAL。

Two oligosaccharides, a hexasaccharide fragment of group E Streptococcus polysaccharide and a tetrasaccharide repeating unit of E. coli O7: K98: H6, consisting of α-(1→2)-and α-(1→3)-linked rhamnose backbones were synthesized in a regio-and-stereoselective manner.

以三氯乙酰亚胺酯为供体，不保护或部分保护的糖为受体，成功地合成了以α-（1→2）-和-α（1→3）-交替连接的鼠李糖为主链的GroupE Streptococcus六糖和E.coli O7：K98：H6四糖。

Transcriptional factors play an equivalent role in plant during drought adaptation and cold acclimation. In order to study the expression pattern of CB F4 gene promoter and explore its potential application in plant genetic engineer ing, CBF4 promoter fragment was amplified by PCR method from Arabidopsis thalian a genome DNA.

根据已有文献及公开的拟南芥基因组序列，利用PCR方法从拟南芥基因组DNA中扩增得到了转录因子CBF4上游的DNA片断，对其进行序列分析表明与GenBank序列高度同源，同时对其进行初步的功能分析。

The hypothesis that H-fragment was a transposible element in thermophilic bacteria was suggested and discussed.

提出了H片段是高温菌CU21中的一个转座因子的假说。

Four thrombogenic and thrombolytic markers including endothelin-1(ET-1), platelet granule membrane protein-140 (GMP-140), thrombin active fragment and D-Dimer were determined before and after ILLLI or aspirin treatment.

观察两组治疗前后血浆中致血栓和纤溶指标内皮素-1、血小板α颗粒膜蛋白140（GMP-140）、凝血酶活性片段及D-二聚体的变化。

To detection of Tilletia controversa Kühn sensitively and accurately, real-time PCR systems were developed. The species-specific primer pair CQUTCK4/CQUTCK5 and probe CQUP1 were designed based on a selected specific fragment (1322 bp) specific for TCK, and the SYBR Green I and TaqMan quantitative PCR detection systems were established with optimized reaction conditions.

建立荧光定量PCR体系以准确灵敏的鉴定小麦黑穗菌(Tilletia controversa Kühn， TCK)根据筛选的TCK独有差异基因片段(1322bp)设计特异性引物对CQUTCK4/CQUTCK5和TaqMan探针CQUP1，建立SYBR GreenⅠ荧光染料法和Taq Man水解探针法定量PCR检测体系，并对体系进行优化。

We may judge from the noble commencement of the Timaeus, from the fragment of the Critias itself, and from the third book of the Laws, in what manner Plato would have treated this high argument.

我们或许可以在《蒂迈欧》中找到这场战争的庄严开端，再在《批评家》的片断中继续寻找，然后去《法律》的第三卷中去寻觅，在这些地方的风俗描述之中，柏拉图必定对这一事件予以更高程度的论证。

Amplified fragment length polymorphism was used to analyze the genetic diversity of 92 Torreya grandis from 6 different populations. Total of 364 loci of the T.grandis genome were examined for molecular variation and 63 loci were polymorphic.

利用AFLP分子标记技术，采用8对引物对6个香榧天然群体的92个个体进行了总基因组DNA水平上的多态性检测，共检测出364个位点，其中多态性位点63个。

The Laccase gene of Trametes versicolor's genomic DNA amplified by PCR using a set of primers that we designed in this study. The amplified fragment was cloned into the vector pUCM-T.

本研究以PCR扩增的方法，从变色栓菌的基因组DNA中扩增出了一预期大小的DNA片断，并将其克隆到了pUCM-T载体上。

RESULTS: The cloned DNA fragment showed 97% nucleotide sequence identity and 95% deduced amino acid sequence identity with the mRNA of rat IgEdependent histaminereleasing factor (or translationally controlled tumor protein) in GenBank.

结果： 测序证实克隆基因与GenBank中大鼠IgE依赖组胺释放因子mRNA序列的一致性为97%，推测的氨基酸序列一致性为95%。

As of Tuesday, Google's results were still censored in China.

截至周二，谷歌的搜索结果仍受中国审查。

In order to make the positive action increase and negative one decrease, the sub-forces of the social factors must be adjusted to form a centripetal force.

在这一过程中，人的主体性发挥是社会有机体健康发展的灵魂。