查询词典 fragment

Six hundred ova were transfered into twenty-nine pseudopregnant recipients. Fifty-five offspring were born from the ova mircroinjected with the Bcl-2 fragment expression cassette.Twelve of them were proved to be gene integration positive by PCR .Four founder mice,two male and two female ,were further confirmed to be transgenic by Southern hybridization.

用BglII+SalI+PvuI三酶切pWBS和用BglII+BamHI双酶切pWCS，分别回收并纯化3.0kb的基因片段WAP-Bcl-2-SV40polyA和3.5kb的基因片段WAP-c-myc-SV40poly，作为目的基因，通过受精卵显微注射技术，分别导入C57BL／6J雌鼠与DBA／2J雄鼠交配的F1代小鼠受精卵的雄原核中，各注射约1000枚，将存活的受精卵各600枚分别移植至29只假孕母鼠输卵管壶腹部内。

Psychrophilic and psychro-tolerant bacteria were cultured and used for screening the cold-active enzymes. Some characteristics of cold-active protease and chitinase were analyzed then. Microbial 16S rDNA clone libraries of deep sea sediments were constructed and studied by PCR-RFLP (Restriction Fragment Length Polymorphism) and phylogenetic analysis based on 16S rDNA sequences.

通过构建沉积物中微生物16S rDNA克隆文库，采用PCR-RFLP分析、DNA-DNA杂交、16S rDNA序列测定以及系统发育分析的方法，研究了西太平洋&暖池&区和东太平洋&结核&区两个不同海区深海沉积物中的微生物多样性和群落结构特征及其与环境的相互关系，得到了一些与&暖池&区环境特点紧密相关的新发现，新认识。

Psychrophilic and psychro-tolerant bacteria were cultured and used for screening the cold-active enzymes. Some characteristics of cold-active protease and chitinase were analyzed then.Microbial 16S rDNA clone libraries of deep sea sediments were constructed and studied by PCR-RFLP (Restriction Fragment Length Polymorphism) and phylogenetic analysis based on 16S rDNA sequences.

通过构建沉积物中微生物16S rDNA克隆文库，采用PCR-RFLP分析、DNA-DNA杂交、16S rDNA序列测定以及系统发育分析的方法，研究了两太平洋&暖池&区和东太平洋&结核&区两个不同海区深海沉积物中的微生物多样性和群落结构特征及其与环境的相互关系，得到了一些与&暖池&区环境特点紧密相关的新发现，新认识。

Resilts In the observation of cervical nucleus pulpous prolapse and free disc fragment s...

结果 在颈椎间盘髓核脱出与游离的观察中FFE序列对纤维环断裂、髓核脱出方向以及神经根受压的发现率均明显优于T1WI、T2 WI；在腰椎间盘髓核脱出与游离的观察中FFE对纤维环断裂的显示率明显优于T1WI、T2 WI；对髓核脱出方向的判定明显优于T2 WI ；对神经根受压情况的判定FFE优于T1WI。

MRI FFE can improve the diagnostic ratio of the extrusion of nucleus pulpous,annular bulge as well as free disc fragment.

MRI快速场回波序列可提高椎间盘突出症的椎间盘纤维环断裂、髓核脱出方向以及神经根受压诊断的准确率。

Resilts In the observation of cervical nucleus pulpous prolapse and free disc fragment showing fibrous circle rupture,extrusion direction and nerve-root pressure in cervical region,and showing fibrous cicrcle rupture in lumbar region,the FFE was better than T1WI,T2WI.In judging extrusion direction and nerve-root pressure,the FFE sequence was better than T2WI and T1WI respectively.

结果在颈椎间盘髓核脱出与游离的观察中FFE序列对纤维环断裂、髓核脱出方向以及神经根受压的发现率均明显优于T1WI、T2WI；在腰椎间盘髓核脱出与游离的观察中FFE对纤维环断裂的显示率明显优于T1WI、T2WI；对髓核脱出方向的判定明显优于T2WI；对神经根受压情况的判定FFE优于T1WI。

Primary culture of goat mammary gland cells could be derived by outgrowth of migrating cells from fragment of tissue. Fibroblast and epithelial cells could be pured according to their different sensibility to trypsin. Complex cultures treated by 0.25% trypsin in Hanks' for 5~7min at 37℃, the dispersed cells were mainly fibroblasts,then the rest treated by 0.15% trypsin-0.02% EDTA in Hanks'for 5~8min at 37℃, the majority cells harvested were mammary epithelial cells.

用组织块培养法可获得良好的山羊乳腺细胞原代培养物；培养的山羊乳腺成纤维细胞比上皮细胞对胰蛋白酶更敏感，据此可在传代过程中将二者分离纯化，获得纯细胞系；混合培养物先用0.25%胰蛋白酶在37℃消化5~7min 所收集的细胞主要为成纤维细胞；再加0.15%胰蛋白酶-0.02鞹A 消化液在37℃继续消化5~6min 所回收的细胞绝大多数为上皮细胞，经过2~3 代，即可得到纯化的乳腺上皮细胞系。

Primary culture of goat mammary gland cells could be derived by outgrowth of migrating cells from fragment of tissue. Fibroblast and epithelial cells could be pured according to their different sensibility to trypsin. Complex cultures treated by 0.25% trypsin in Hanks' for 5~7min at 37℃, the dispersed cells were mainly fibroblasts,then the rest treated by 0.15% trypsin-0.02% EDTA in Hanks'for 5~8min at 37℃, the majority cells harvested were mammary epithelial cells.

用组织块培养法可获得良好的山羊乳腺细胞原代培养物；培养的山羊乳腺成纤维细胞比上皮细胞对胰蛋白酶更敏感，据此可在传代过程中将二者分离纯化，获得纯细胞系；混合培养物先用0.25%胰蛋白酶在37℃消化5~7min 所收集的细胞主要为成纤维细胞；再加0.15%胰蛋白酶-0.02%EDTA 消化液在37℃继续消化5~6min 所回收的细胞绝大多数为上皮细胞，经过2~3 代，即可得到纯化的乳腺上皮细胞系。

It can be concluded that the cloned sequence is putatively hsp70a cDNA fragment from Dunaliella salina.

所克隆的序列为盐藻hsp70a cDNA 片段。

Substituent effect of pyrazoles was also investigated by DNMR and DFT.We successfully synthesized these intermediates with exclusively-conformations in a mild condition. X-ray results show that substituents in the pyrazolyl ring affect greatly on the structures of products, especially theπdelocation of the fragment 1-metal-butadiene.

本文以取代吡唑和1-炔基Fischer卡宾化合物的Michael加成反应合成了12个-型β-吡唑基烯基卡宾化合物，反应条件温和。X-ray晶体衍射显示吡唑上取代基对产物结构，特别是1-M-丁二烯的共轭程度具有较大的影响。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。