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At its worst the pietistic tendency can lead to inordinate subjectivism and emotionalism; it can discourage careful scholarship; it can fragment the church through enthusiastic separatism; it can establish new codes of almost legalistic morality; and it can underrate the value of Christian traditions.

在其最坏的pietistic趋势可能会导致过度的主观主义和感情主义，它可以阻止认真奖学金；它可以教会片段通过热情的分裂主义，它可以建立新的守则，几乎法律道德；它可以低估价值的基督教传统。

After induced with IPTG, the proteins expressed by different fragments were analyzed by SDS-PAGE, and the results showed that all these truncated proteins could be expressed in E. coli BL21. Bioassay result against Plutella xylotella showed that the active fragment of Cry1Ba3 protein was located between amino acid No. 22 and No. 655. As the insecticidal activity of the truncated protein containing amino acid No. 54-655 was declined significantly, and No. 22-627 and No.

采用浸叶法检测这些蛋白片段对小菜蛾的杀虫活性，研究结果表明含有第1～685位和含有第22～655位氨基酸的蛋白片段对小菜蛾的毒性，与全长Cry1Ba3蛋白相比，没有改变；含有第54～655位氨基酸的蛋白片段对小菜蛾的毒力明显降低；而含有第22～627位和含有第85～655位氨基酸的蛋白片段完全丧失了对小菜蛾的活性。

Restriction map of H. polymorpha CBS 1976 chromosomal locus containing △9-fatty acid desaturase gene was constructed based upon the data of Southern analysis, and a 3. 4kb BamHⅠ-XhoⅠ genomic fragment was isolated. Sequence analysis showed that it contains an ORF of 1353bp with high homology with cloned yeast △9-fatty acid desaturase genes.

构建了H.polymorpha CBS 1976染色体△9-脂肪酸去饱和酶基因座位的限制性酶切图谱，进而分离了3.4kb BamHⅠ-XhoⅠ基因片段并进行全序列分析，结果表明这个片段含1个与已克隆的酵母△9-脂肪酸去饱和酶基因高度同源的、由1353bp组成的ORF。

If by means of a prereduction MRI or after neurologic deterioration during closed reduction, the surgeon becomes aware of a fragment of disc material displaced into the spinal canal, then an anterior discectomy, open reduction, fusion, and plating becomes necessary.2,7,9 In approximately half of the cases, a closed reduction will be successful in reducing the dislocation before surgery.50 In the majority of the others, an intraoperative reduction performed after the discectomy and decompression will achieve anatomic reduction.9,51 For those patients in whom an anterior discectomy and decompression have not been successful in reducing the dislocation, then a supplemental posterior open reduction should be performed.52

假如借助MRI检查，或在闭合复位过程中神经症状进一步加重，外科医生意识到有椎间盘碎片突入了椎管，此时就需要前路椎间盘切除减压、开放复位和固定融合。大约有一半的病例手术以前能够通过闭合复位来成功的将脱位复原，剩下的病例则需要在术中将椎间盘切除减压来达到解剖复位。假如有病人经过前方的椎间盘切除和减压后仍然不能成功的还原脱位，则需要再行后方的开放复位术。

Therefore, there're only single charged parent ions of the sample gas molecule under the condition of photons' irradiation effect, and no fragment ions produced. Consequently, the mass spectra obtained from photoionization is very simple, and the sample gas can be detected without preseparation.

样品气体分子在光子的照射作用下只产生单电荷母离子，不产生碎片离子，以该电离方式获得的质谱图非常简单，因此气体样品不需分离即可进行检测。

YAC is now prevailingly used as the first cloning vector for human genome analysis, but one problem that is not yet solved is the insert DNA fragment is not easily isolated from yeast chromosomes and vectors. So, we developed a new technique for the specific radioactive labelling of YAC insert by Alu-PCR technique.

因目前YAC中的插入片段还无法分离出来，因此我们对筛选到的这些YAC，用Alu-PCR技术将人基因组插入片段进行了特异扩增，并通过PCR条件的适当改变，获得较理想的标记效果。

Objective: To study the effects of recombinant expression vector containing human breast cancer DF3 promotor and diphtheria toxin A fragment on human breast cancer cells.

目的：研究新构建的含人乳腺癌DF3启动子和白喉毒素A片段的重组表达载体PGL3-DF3-DTA对人乳腺癌细胞的影响。

This experiment aimed to systematically investigate and analyze the ethanol activated rates of mouse oocytes, affected factors of oocytes activation, types and development of parthenotes, and the organization and roles of the cytoskeleton during pronuclear formation and migration and early embryo cleavage by ethanol activation of oocytes, embryo culture in vitro and immunofluorescence cyto-chemistry. The results:(1) The ethanol activation of mouse oocytes showed that the activated and developmental rates of oocytes increased significantly with the increase of ethanol concentration and extension of exposure time, but over concentration and exposure time would result in increased fragment rates significantly. 7% ethanol treated oocytes for 7min was the optimum activated condition.

本实验主要利用卵母细胞的乙醇激活、胚胎体外培养和免疫荧光细胞化学方法对小鼠卵母细胞乙醇激活效率、影响因素、孤雌胚类型、发育、原核形成、迁移及早期胚胎卵裂过程中细胞骨架的组装、作用等进行了系统的研究和分析，结果显示：(1)小鼠卵母细胞的乙醇激活结果表明，随着乙醇浓度的升高和作用时间的延长，卵母细胞激活率和发育率都显著提高，但乙醇浓度过高和作用时间过长会导致卵母细胞碎裂率的显著增加，7%乙醇作用卵母细胞7min 为最佳激活条件。

The tryptophan auxotrophs and the kanamycin resistant prototrophic revertants have been proved to contain a copy of Tn5 inserted in the chromosome by Southern Blot analysis with DIG-labeled 3. 5 kb HindⅢ fragment of pkan2 as the Tn5 probe. Four typies of prototrophic revertants could be found by the above assay.

用Tn5探针进行的Southern分子杂交证明Tn5在色氨酸营养缺陷型菌株及部分Km抗性原养回复突变型菌株中均插入在染色体DNA上，并且对菌株本身具有的内源性质粒的数目及大小没有带来影响。

Methods Purified 5-LO fragment was injected into male pronucli and the firtilized eggs were transplanted into pseudopregnant mice. PCR and Southern blot were used to detect the genotype of DNA separated from the newborn mouse tail tissues. RT-PCR and Western blot analysis were used to detect the gene transcription and expression. Results PCR and Southern blot results showed that 7 of 25 mice were transgenic mice.

通过显微注射的方法，将5-脂氧化酶基因片段(6.8 kb)导入BDF1受精卵雄原核并移植到同期受孕的假孕母鼠输卵管中，对产出仔鼠的鼠尾组织DNA进行PCR、Southern blot检测，对9、20、24号转基因小鼠分别提取腹腔细胞、骨髓细胞及脾、肾组织总RNA和蛋白，并采用RT-PCR、Western blot方法进行转录水平检测和蛋白表达检测。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。