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To validate an investigation method for the epidemiology of Clostridium perfringens in chicken,genetic diversities of 34 strains of C.perfringens type A isolated from healthy chicken of 10 diffe-rent integrated chicken farms in Sichuan Province were analyzed by repetitive extragenic palindromic PCR and amplified fragment length polymorphism methods.

为探讨鸡产气荚膜梭菌流行现状的调查方法,采用REP-PCR(repetitive extragenic palindromic PCR)技术对四川省10个规模化鸡场分离得到的34株A型产气荚膜梭菌进行了遗传多样性分析,并与AFLP(amplified fragment length polymorphism)方法进行了分型比较。

Objective To detect DNA polymorphism of Papaver somnferum L using fluorescent Amplified Fragment Length Polymorphism.

目的 利用荧光AFLP检测技术检测罂粟植物DNA多态性。

This method could amplify the specific fragment in the reference strains, but not in chicken E. coli, Haemophilus paragallinarum, Salmonella Pullorum and Avian pasteurella multocida, of ORT. A sensitivity test indicated that the assay's lowest detection limit was 90 pg DNA.

该方法能在ORT参考菌株中扩增到特异性片段,而鸡大肠杆菌、副鸡嗜血杆菌、鸡白痢沙门氏菌、禽多杀性巴氏杆菌的扩增结果均为阴性;敏感性试验表明该方法最低检出限量为90 pg DNA。

The use of locked periarticular elbow plates improves the stability of the fixation and allows fragment specific angle-stable fixation especially with the comminuted C3 types.23 The exposure needed for this fixation is similar to conventional distal humeral double plating.

使用肘关节周围锁定钢板提高了固定的稳定性,并允许片段具体角稳定固定尤其是粉碎性C3型骨折。为这一固定所需的暴露类似常规肱骨远端双重钢板。

One endophytic bacteria strain LP-5 which could significantly inhibit some phytopathogenic fungi was isolated from xylem of pear. According to morphology, physicochemical test, and analysis of the 16S rDNA sequence and specific amplified fragment, the strain was identified as Bacillus amyloliquefaciens.

从梨树木质部分离到1株具有强烈抑菌活性的内生细菌LP-5,通过形态观察、生理生化测定、16S rDNA序列分析及特异基因片段扩增,将其鉴定为解淀粉芽孢杆菌。

At its worst the pietistic tendency can lead to inordinate subjectivism and emotionalism; it can discourage careful scholarship; it can fragment the church through enthusiastic separatism; it can establish new codes of almost legalistic morality; and it can underrate the value of Christian traditions.

在其最坏的pietistic的趋势,可导致过度的主观主义和情;它可以阻止小心奖学金;片段,它可以教会通过热烈的分裂主义,它可以建立新的行为守则,几乎法律道德;并且可以低估的价值,基督教传统。

At its worst the pietistic tendency can lead to inordinate subjectivism and emotionalism; it can discourage careful scholarship; it can fragment the church through enthusiastic separatism; it can establish new codes of almost legalistic morality; and it can underrate the value of Christian traditions.

在其最坏的pietistic趋势可能会导致过度的主观主义和感情主义,它可以阻止认真奖学金;它可以教会片段通过热情的分裂主义,它可以建立新的守则,几乎法律道德;它可以低估价值的基督教传统。

After induced with IPTG, the proteins expressed by different fragments were analyzed by SDS-PAGE, and the results showed that all these truncated proteins could be expressed in E. coli BL21. Bioassay result against Plutella xylotella showed that the active fragment of Cry1Ba3 protein was located between amino acid No. 22 and No. 655. As the insecticidal activity of the truncated protein containing amino acid No. 54-655 was declined significantly, and No. 22-627 and No.

采用浸叶法检测这些蛋白片段对小菜蛾的杀虫活性,研究结果表明含有第1~685位和含有第22~655位氨基酸的蛋白片段对小菜蛾的毒性,与全长Cry1Ba3蛋白相比,没有改变;含有第54~655位氨基酸的蛋白片段对小菜蛾的毒力明显降低;而含有第22~627位和含有第85~655位氨基酸的蛋白片段完全丧失了对小菜蛾的活性。

Restriction map of H. polymorpha CBS 1976 chromosomal locus containing △9-fatty acid desaturase gene was constructed based upon the data of Southern analysis, and a 3. 4kb BamHⅠ-XhoⅠ genomic fragment was isolated. Sequence analysis showed that it contains an ORF of 1353bp with high homology with cloned yeast △9-fatty acid desaturase genes.

构建了H.polymorpha CBS 1976染色体△9-脂肪酸去饱和酶基因座位的限制性酶切图谱,进而分离了3.4kb BamHⅠ-XhoⅠ基因片段并进行全序列分析,结果表明这个片段含1个与已克隆的酵母△9-脂肪酸去饱和酶基因高度同源的、由1353bp组成的ORF。

If by means of a prereduction MRI or after neurologic deterioration during closed reduction, the surgeon becomes aware of a fragment of disc material displaced into the spinal canal, then an anterior discectomy, open reduction, fusion, and plating becomes necessary.2,7,9 In approximately half of the cases, a closed reduction will be successful in reducing the dislocation before surgery.50 In the majority of the others, an intraoperative reduction performed after the discectomy and decompression will achieve anatomic reduction.9,51 For those patients in whom an anterior discectomy and decompression have not been successful in reducing the dislocation, then a supplemental posterior open reduction should be performed.52

假如借助MRI检查,或在闭合复位过程中神经症状进一步加重,外科医生意识到有椎间盘碎片突入了椎管,此时就需要前路椎间盘切除减压、开放复位和固定融合。大约有一半的病例手术以前能够通过闭合复位来成功的将脱位复原,剩下的病例则需要在术中将椎间盘切除减压来达到解剖复位。假如有病人经过前方的椎间盘切除和减压后仍然不能成功的还原脱位,则需要再行后方的开放复位术。

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As of Tuesday, Google's results were still censored in China.

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