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In conclusion, a prokaryotic plasmid, encoding the fusion gene of single-chain variable fragment with MCP-3 and expressing idiotype protein vaccination against B cell lymphoma, was constructed correctly.

本研究成功构建鼠源scFv片段与趋化因子MCP-3融合的独特型B细胞淋巴瘤疫苗表达质粒pGLo/scFv- MCP-3，并实现融合蛋白的初步表达。

The recombinant plasmids were analyzed by sequencing and the one containing the correct fragment was transfected into K562 cells. The condition of idiotypic protein expression was tested by Western-Blot.

将经测序鉴定正确的重组质粒转染K562细胞，Western-Blot等方法检测其蛋白表达的情况。

Sees only Moon crater which this at the beginning of war rests, from piece of on pale under black light and shade lamination dust, the slowly appearance, under the Moon small gravitation, true completely is being in the clouds quiet down, estimated needs at least for month-long the time, looked like the war the haze also must continue for a long time; Harbors the extremely serious mood, greatly leads several intimate assistants for doctor, passed through the thick dust dense fog, in basically cleans in the battlefield which finished to seek, the human brave warrior's skeleton already properly is processed early, but in the battlefield everywhere all has without enough time cleaning up each kind of weaponry, each kind of components which the guns, the bullet, the portable laser cannon and scatter, mixes with in the every large or small chippings, eliminates this also to have is wrecked innumerably, the kind of person robot makes threatening gestures to maintain each kind of death posture wreckage and the fragment, this gets down quietly the battlefield everywhere is filling the air the death breath, is constraining the people sensitive frail nerveDavid Dr.

只见这座大战初歇的月球环形山，正在从一片上淡下黑明暗分层的尘埃云中，慢慢的显露出来，在月球较小的引力作用下，真正的完全沉寂下来，估计至少需要一个月的时间，看来战争的阴霾还要持续许久；怀着极为沉重的心情，大为博士带领几位亲密的副手，穿过浓厚的尘埃迷雾，在基本打扫完毕的战场上寻找着，人类勇士的尸骸早已经被妥善处理，但是战场上到处都有来不及清理的各种武器装备，枪支、子弹、便携激光炮和散落的各种零件，夹杂在大大小小的碎石块中，除此还有无数被击毁的，类人机器人张牙舞爪保持着各种死亡姿态的残骸和碎片，这个沉寂下来的战场到处弥漫着死亡的气息，压抑着人们敏感脆弱的神经，大卫博士一行人，就这样巡视着并细心察看着这场战争的严重破坏。

Irrigable land was the main type of cultivated land, the class metrics showed the increase of the fragment and the dispersed degree et al.

水浇地作为耕地中的主要类型，表现为破碎度增加，斑块间离散度增加等方面。

Completion of the synthesis involved displacement of the remaining alcohol through a three-step, telescoped sequence featuring an efficient cleavage of an aryl mesylate, and a practical method for introduction of the isobutyric acid fragment.

完成剩余的位移所涉及合成酒精通过三个步骤不胜顺序展出了高效率的卵裂甲磺酸芳、而实际的方法引入异丁酸片段。

To determine differential expression of genes between near isogenic lineMardler/7*Bainong 3217 and Bainong 3217,cDNA-AFLP was employed to assay gene expressed in different time points after inoculation with powder and different treatments.

采用cDNA-AFLP(cDNA-amplified fragment length polymorphism)技术，对小麦抗白粉病近等基因系Mardler/7*百农3217和百农3217材料的不同处理，于接菌后不同时间点的基因表达进行了表达分析。

Some characteristics of unmodified mAbs, such as average half-life, vary with the isotype, which is determined by the crystallizable-fragment region.

未改变的单抗的部分特性，如平均半衰期，随单抗型的不同而不同，这主要取决于其分子的结晶区。

Methods 202 kinless subjects of the Chinese Han were investigated in this study, including 82 normal controls, and 120 cases diagnosed as CHD. The PPARδ+294T/C gene polymorphism was determined by polymerase chain reaction and restriction fragment length polymorphisms. The frequencies of PPARδ+294T/C genotypes and the "C"allele frequency were analyzed, to evaluate the risk for the CHD among variant genotypes.

运用聚合酶链式反应及限制酶片段长度多态性技术分析无血缘关系汉族人群[包括82例正常对照者，120 例冠心病患者]的PPARδ+294T/C基因多态性，分析基因型频率、等位基因频率，并对不同基因型患者冠心病的危险性进行评价。

Lamblia was extracted and a full-length variant-specific surface antigen gene fragment was amplified by polymerase chain reaction.

提取蓝氏贾第鞭毛虫基因组DNA，PCR扩增表面变异抗原基因片段。

Results The full-length variant-specific surface antigen gene fragment from G. lamblia was found to be 2 142 bp, encoding a 713 amino acid polypeptide and contained a single open reading frame.

结果 中国人源蓝氏贾第鞭毛虫基因全长为2 142 bp，编码1条长为713 个氨基酸残基的多肽链，含有一个简单的开放阅读框。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。