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The massive ogre grunted and shoved, his enormous shoulder pushing the last Runestone fragment another foot across the thickly grassed clearing.

这个巨大的食人魔发出哼一哼的声音，他强健的胳膊正举着最后一块符文石碎块走在这块空地上。

Methods Spermatocytes, early stage spermatids and the later stage spermatids were separated from the adult rat testis by the velocity gravitative sedimentation technique. Using the whole mount electron microscopy, SDS-PAGE and Southwestern blot, the morphology, components of NM-L-IF and the relationship between an A-T repetitive DNA fragment and the proteins of NM-L-IF in those cells were analyzed.

利用整装电镜技术、SDS-PAGE和Southwestern blot等技术观察与分析经单位重力沉降法分离的大鼠精母细胞、早期精子细胞和晚期精子细胞的NM-L-IF的形态结构、蛋白组成及其与A-T重复序列的相互关系。

The key research progresses and results that we accessed by the project were as follows: 1 A pair of degenerated primers were designed in the conserved domain which based on the amino acid sequence of HAK in Arabidopsis thaliana. The 500 bp fragment of HAK were amplified from the roots of Puccinellia tenuiflora by RT-PCR. The full sequence of HAK were obtained by 5' and 3' RACE methods and were determined and analyzed.

本项目主要取得了以下研究进展和成果：（1）根据已有HAK转运蛋白氨基酸序列在保守区域设计简并PCR引物，用反转录PCR方法，扩增出500bp的小花碱茅根HAK片段，通过5''和3''RACE的方法获取了该HAK的全长序列，完成了测序和序列同源性分析。

Sequence comparison showed that the deduced amino acid of the partial fragment of catalase gene from F. chinensis shares 95% and 73% identity with that of Macrobrachium rosenbergii and Haliotis discus, respectively.

多序列比对结果发现目前所得中国明对虾Catalase基因部分片段的推导氨基酸序列与罗氏沼虾和皱纹盘鲍Catalase氨基酸序列的同源性分别达到95%和73%。

False positive were lowered to an acceptable rate by this protocol, therefore, a big amount of work on fingerprint to get rid of false positive clones can be avoided. When whole BAC clone containing one locus of a multi-loci probe were applied for library screening, no matter homogenic BAC or heterogenic BAC, site-specificity is higher than short DNA fragment probes.

对于无法通过小基因组填补间隙的区段，可以本物种BAC克隆直接进行染色体步移，采用抑制重复顺序杂交法能够有效抑制大量存在于植物基因组中的重复顺序的干扰，把假阳性率降低到可以接受的范围，从而避免了用大量指纹分析剔除假阳性克隆。

Using the homogene cloning methed, we cloned a cDNA fragment of the CDPK from Dunaliella.

本实验研究的内容主要包括以下四个方面： 1。

The two Epistles to the Thessalonians are included among the canonical books accepted by the Councils of the Vatican, of Trent, and of Florence, and are among the homologoumena of all early lists of canonical New-Testament Scriptures; for instance, to mention only such early lists as accord with the received canon of Trent, these two Epistles are listed in the Muratorian Fragment (AD 195-205), in the canons of St.

双方的撒罗书信中有规范的图书接受安理会的梵蒂冈的特伦特和佛罗伦萨，并在所有早期homologoumena名单规范新旧约圣经，例如，只提及诸如早期名单符合收到佳能的遄达，这两个书信中列出穆拉多利片段（公元195-205 ），在大炮的圣亚他那修亚历山大（公元373 ），第三次理事会迦太基（公元397 ），其中参加了圣奥古斯丁，圣埃皮法尼乌斯（公元403 ），我的清白（公元405 ），和格拉西（公元492 ）。

Objective To explore the clinical value of renal dynamic imaging and urinary N-acetyl-β-D-glucosaminidase, apoplosis DNA fragment in evaluating the damage degree of hydronephrotic kidneys in children with hydronephrosis.

目的 探讨核素肾动态显像单光子发射计算机断层扫描仪检查与肾盂尿N-乙酰－β-D-氨基葡萄糖苷酶、凋亡DNA片段水平检测在评定肾积水患儿病肾损害中的临床价值。

In this study, we refer to the NCBI database to design primers which are used for amplification of the gene fragment of hepatopancreatic parvovirus、infectious hypodermal and hematopoietic necrosis virus and taura syndrome virus.

本研究从NCBI database资料中，分别在肝胰小病毒(hepatopancreatic parvovirus， HPV)、传染性皮下及造血组织坏死病毒(infectious hypodermal and hematopoietic necrosis virus， IHHNV)和桃拉病毒(taura syndrome virus， TSV)等三种常见之虾病毒中，各选出一段壳蛋白基因，利用重组蛋白的方式，在大肠杆菌中大量表现。

In this study,Amplified fragment length polymorphism analysis was carried out by using 23 pairs of EcoRI-NN/MseI-NNN selective primers in amplification of the DNAs from reciprocal F_1 hybrids of Cucumis hystrix Chakr.and C.

为了研究细胞质背景对基因组序列丢失的影响，利用23对Eco RI-NN/Mse I-NNN选择性引物对Cucumishystrix与栽培黄瓜C.sativus的正反交F_1、异源双二倍体及亲本DNA进行AFLP分析。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。