查询词典 fragment

AIM: To determine whether immunization with recombinant filarial chitinase or a fragment containing the epitope recognized by McAbMF1 and SXP 1 could protect jirds against microfilaremia resulting from infection with B.malayi.

目的：测定重组马来丝虫壳质酶（r－chitinase）及其片段和SXP－1抗原免疫接种沙鼠后是否具有抗马来丝虫微丝蚴血症的保护性免疫作用。

The 7.4 kb DNA fragment containing the S. Choleraesuis std fimbrial gene cluster was PCR amplified and cloned into the pCR-XL-TOPO vector. The open reading frames of the std fimbrial gene cluster were analyzed. The resulting recombinant DNA was transformed into Escherichia coli HB101 strain.

本实验室藉由PCR方式，增幅7.4 kb的std线毛基因组，将其选殖至pCR-XL-TOPO质体DNA，委外定序后，分析std线毛基因组之开放性读框，进而将此重组DNA送入大肠杆菌HB101，并藉由RT-PCR及西方点墨试验观察到S。

Basic formule on the quantitative anaiysis of fragment microfabrics were derivated.

推导了进行碎屑定量微组构分析的基本公式。

The Cambridge fragment, seventh century, discovered in a Cairo genizah.

剑桥片段，七世纪，发现在开罗

The results of comparative analysis on NCBI-BLAST indicated that the cloned fragment contained 812 nucleotides and shared a homology of 90% with the reported sequence of germin gf-2.8 gene while its amino acid sequence shared a homology of 96%,A majority of the nine differences between and among the two sequences involve equivalent amino acids.

氨基酸序列的同源性为96％，其中有九个氨基酸不同，但基本上为同等氨基酸，并且符合germin中三组氨酸和一谷氨酸的草酸氧化酶活性位点的同源模型特征。

The three conserved histidine residues with a conserved glutamate residue fits the active site of the homology model of oxalate oxidase.2. Construction of plant binary expression vector of germin geneThe germin gene fragment cut with BamH I from intermediated vector pDG28 was inserted into binary vector pBI121 cut with the same enzyme to formrecombinants which was selected by plaque PCR .

Germin基因植物表达载体的构建将中间载体上的germin基因片段用Bam H I酶切下来，插入用同样酶切的双元表达载体pBI121上，以菌落PCR筛选重组子。

Drug sensitive test and three-dimensional test220 strains of Pa were isolated from hospitalized patients between 2003 and 2007. K-B method was used to tested the susceptibility of 10 different antibiotics. IRPa was screened by testing the minimal inhibitory concentration of imipemem by using agar diluiion method.The susceptibility of these IRPa to the antibiotics was analysised. Three-dimensional test was used to identify the different kinds of beta lactamases from 220 strains of Pa.2.Carbarpenems hydrolytic enzyme genes and oprD2 gene were detectedamong the selected IRPa strains, PCR method was performed to detect carbapenemase genes which included GES、KPC、SPM、VIM、IMP、GIM gene and the oprD2 gene;Multiplex PCR were used to detect OXA genes and plasmid-mediated AmpC beta lactamase genes; The expression of the chromosomal AmpC beta lactamases and oprD2 genes in IRPa strains were analyzed by Real-time PCR.3.Identification and characterization of integronsIntegrase gene was detected by PCR, and the classification of integrons was performed by using restriction fragment length polymorphism.PCR was performed to detect the qacE△1-sull gene,and the gene cassetes which are located at variable region of integrons in the strains were detected to be positive.

方法1、药敏实验和三维实验收集2003～2007年临床分离的220株Pa，对这些菌株采用K-B法测定10种临床常用抗生素的药敏情况，同时采用琼脂稀释法检测亚胺培南的最低抑菌浓度(Minimal inhibitory concentration，MIC)，筛选出对亚胺培南耐药的铜绿假单胞菌，并分析其对其它抗生素的药物敏感率；采用三维实验的方法分析220株Pa产β内酰胺酶的类型。2、碳青霉烯类水解酶和oprD2蛋白的检测针对鉴定的IRPa菌株，采用普通PCR方法检测具有碳青霉烯水解作用的β内酰胺酶耐药基因(GES、KPC、SPM、VIM、IMP、GIM基因)和oprD2基因，采用多重PCR的方法检测OXA型基因和质粒携带的AmpC酶基因，用荧光定量RT-PCR方法检测oprD2蛋白基因表达情况；同时对产AmpC酶的Pa(25株，含IMP耐药和敏感株)用RT-PCR方法检测AmpC酶基因的表达量情况。3。

Labral fragment. Hypointense middle glenohumeral

显示撕脱的前唇碎片向前移位，低信号的中盂肱韧

According to the nucleotide sequences, one pair of PCR primers were designed and synthesized to detect the femalespecific fragment in gonochoristic offspring.

根据所测序列设计的一对引物经PCR检测也证实该片段为雌性特异。

The Gordonia specific 16S rDNA fragment (829 bp or 436 bp) was successfully amplified for all the reference Gordonia species with the designed primer pair G268F/G1096R or G699F/G1134R whereas, no amplification was noted for closely-related species from other genera.

研究结果显示，利用G268F/G1096R及G699F/G1134R两组Gordonia菌属特异性引子，可有效区别Gordonia菌属与其他放线菌菌属，并且利用此侦测技术已从台湾分离出36株Gordonia分离株。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。