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Objective: To study anti-tumor mechanism of chimeric anti-CD20 antibody fragment Fab' in vitro .

目的：探讨嵌合抗CD20抗体Fab'片段的抗肿瘤机制。

We used the degenerated primers PCR to produce a cDNA fragment from total RNA of Chlamydomonas reinhardtii.

利用简并引物PCR和RACE从衣藻中扩增得到了一个ftsZ基因的部分cDNA序列，命名为CrFtsZ。

The Km and Vmax were 7.35 mg/ml and 0.206 nmol/min-mg for ChoA, and 30.42 mg/ml and 0.376xmol/min-mg for ChoB, respectively. A 270bp DNA fragment, the partial chitosanase gene from Penicillium sp.

酶A的米氏常数K_m及最大反应速度V_分别为7.35 mg／ml、0.206 μmol／min·mg，酶B的米氏常数K_m及最大反应速度V_分别是30.42 mg／ml、0.376μmol／min·mg。

According to amino acid sequence to vitellin which has the structural character of GL/ICG motif near C end, two degenerate primers were designed and Chrysopa septempunctata s RNA was extracted, and a 900bp fragment was amplified by RT-PCR.

根据昆虫卵黄蛋白氨基酸序列在靠近C端存在GL／ICG区域的结构特点，设计了两条简并引物，提取大草蛉的RNA，用Takara的3′—RACE试剂盒进行RT-PCR，扩增出一段900bp的片断。

Objective To express the N-terminal fragment of human mast cell chymase in E.coli and prepare its polyclonal antibody from mouse.

目的：在大肠杆菌中表达人肥大细胞类糜蛋白酶N端片段，并制备其鼠源性多克隆抗体。

The total RNA from brain tissue of substantia nigra of PD rats is extracted at the 1st, 2nd, 4th and 8th week and comparison is made between it and that from normal rats. The total RNA, after RNA formaldehyde degenerated sepharose electrophoresis and separation and Northern transfer, is adsorbed unto colloxylin membrane. Then ECL labeled Selenoprotein P and an unknown gene fragment are used as probes to hybridize with the RNA on the colloxylin membrane. And the hybridized result is obtained with enzyme-linked immunosorbent assay. Finally, analysis of OD is made using ONE-Dscan software.

分别提取1周、2周、1月、2月的帕金森大鼠（各8只）黑质区脑组织总RNA，并取相对应时间的正常大鼠的脑组织总RNA作为对照，经RNA甲醛变性琼脂糖凝胶电泳分离后，通过Northern转移，将其吸附在硝酸纤维素膜上，然后利用ECL标记的Selenoprotein P和一段未知基因为探针，与硝酸纤维素膜上的RNA分子进行杂交，再通过显影取得杂交的结果，应用ONE-Dscan软件进行光密度值分析。

Taking the Lagrange algorithm of LS-DYNA, selected the Johnson-Cook material model which was compatible with metal penetrating problem, the finite element model of columniform fragment penetrating steel target is created.

中文摘要：采用LS-DYNA的Lagrange算法，选择了适合金属侵彻问题的Johnson-Cook材料模型，建立了圆柱形破片侵彻钢质靶板的有限元模型。

Then the combinant plasmid was conducted into ACC-2 cell by electroporation. ACC-2 cells stably expressing CD was obtained by 10-day positive selection with 400 μg/mL G418. Total RNA was extracted and the expression of the CD gene in transfected ACC-2 cells was identified by RT-PCR. RESULTS: PCR yielded a fragment of l280bp and CD was verified by sequence analysis.

测序正确后，将其亚克隆到质粒表达载体pIRES中，构建以内部核糖体进入位点相连的CD基因的质粒表达载体pIRES-CD，采用电穿孔法，以质粒表达载体转染ACC-2细胞，用400μg/mL的G418筛选10d，获得稳定表达CD基因的ACC-2细胞系，提取该细胞的总RNA，用RT-PCR检测CD基因的表达。

The radial head was excised and was not replaced, and the fixation of the coronoid fragment was unsuccessful.

桡骨头被切除，没有置换，冠状突骨块固定失败。

This includes (1) fixation or replacement of the radial head,(2) fixation of the coronoid fragment,(3) repair of the lateral collateral ligament complex, and (4) repair of the medial collateral ligament and/or application of a hinged external fixator for patients who demonstrate residual instability.

该方法包括：桡骨头复位或置换，冠状突骨块的复位，侧副韧带的修复以及修复内侧副韧带或对些肘关节不稳定的患者应用外固定架。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。