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In order to investigate whether fragments of Sugarcane bacilliform virus were integrated into Saccharum inter-specific hybrids genome, a DNA fragment homologous to SCBV was amplified from a hybrid sugarcane plant Saccharum inter-specific hybrids var.

为研究甘蔗杆状病毒Sugarcane bacilliform virus的基因组片段是否整合进杂种甘蔗Saccharum inter-specific hybrids基因组,以采自广西甘蔗研究所的1株杂种甘蔗植株叶片总DNA为模板,根据已报道的甘蔗杆状病毒基因组序列设计多条特异引物。

In addition to Fe+(54Fe+, 56Fe+, 57Fe+), other fragment ions were detected in bacteriogenic pyrites, such as 27 Al+, Si+(28Si+, 29Si+, 30Si+), 40Ca+, Cu+(63Cu+, 65Cu+), Zn+(64Zn+, 66Zn+, 67Zn+, 68Zn+), Ni+(58Ni+, 60Ni+, 62Ni+), and C3H7+.

在杆状菌落成因的黄铁矿中,检测到的组成有 Fe+(54 Fe+、56 Fe+、57 Fe+)、 27 Al+、 Si+(28 Si+、29 Si+、30 Si+)、40 Ca+、 Cu+(63 Cu+、65 Cu+)、 Zn+(64 Zn+、66 Zn+、67 Zn+、68 Zn+)、 Ni+(58 Ni+、60 Ni+、62 Ni+)、 C3H7+。

After treatment of benzamide which is an inhibitor of poly polymerase,β-galactosidase activity assays and PCR analysis were performed to test the deletion of LacZ gene and c-Ha-T24ras gene. The results showed that:(1)LacZ gene was deleted from both A13.4-NIH3T3 cells and B12.7-NIH3T3 cells, but neigher from A13.4-HeLa cells nor B12.7-HeLa cells. So it is possible that there is cell line specificity in gene deletion induced by BA.(2) In A13.4-NIH3T3 cells, LacZ gene and c-Ha-T24ras gene were completely deleted at the same time.(3) The transcription activity of exgenous DNA fragment may have same effect on its sensitivity to the deletion induced by BA.

用聚ADP核糖聚合酶的NAD位点抑制剂苯甲酰胺分别处理四种转化细胞后,检测细胞中整合的外源LacZ基因与c-Ha-T24ras基因的删除情况,结果如下:BA诱导的基因删除可发生于NIH3T3转化细胞系,但不能发生于HeLa转化细胞系;位于同一外源表达载体上的LacZ基因与c-Ha-T24ras基因的删除过程是同步的;外源整合DNA片段的转录强度可能直接影响其被BA诱导删除的敏感性。

Replacement of one benzimidazole fragment with benzothiazole or benzoxazole and optimization of the structure to create the new frame of the compounds were also carried out with the exception to find more potent compounds. The purpose of design and synthesis of these compounds is to investigate the QSAR of this class of compounds with the inhibition of HCV NS3/NS4A protease and eventually to develop more potent inhibitors.

本文首次采用了丙二酰胺与邻芳二胺在微波辐射下制备对称性双芳并唑甲烷化合物、用芳并唑-2-乙酸乙酯与邻芳二胺在微波辐射下制备非对称性双芳并唑甲烷化合物、用4-取代噻唑-2-乙酸乙酯与邻芳二胺在微波辐射下制备非对称性(4-取代-噻唑-2-基)(1H-芳并咪唑-2-基)甲烷化合物等合成路线,共合成了48个目标化合物,其中41个为新化合物,用~1HNMR,ESI-MS,FT-IR,元素分析对这些化合物进行了全面表征,确定了这些化合物的结构,并对部分化合物进行了~(13)CNMR分析。

Then Bink stubbed his toe on a sharp fragment of stone.

宾克的脚趾头踢到了一块锋利的碎石块。

Mitochondria DNA extracted from bivoltine silkworm pupae was digested by EcoRⅠ into five restriction fragments,of which the 1.9kb fragment was cloned and sequenced.

克隆了二化性家蚕蛹体的线粒体DNA EcoRⅠ酶解1.9kb片段,并进行了序列测定。

The total RNA was abstracted from the larvae of Boophilus microplus, and a 1982bp Bm86 gene was amplified by RT-PCR. The target gene was subcloned into T vector. The sequencing showed that the nucleotide sequence of the cloned Bm86 gene shared 97.4% homology with the data published in and this fragment contained the complete open reading frame of Bm86 gene.

为了克隆微小牛蜱Bm86 基因及构建该基因的表达载体,以微小牛蜱饥饿幼蜱的破解物提取的总RNA为模板,参照已发表的微小牛蜱Bm86基因的核苷酸序列,设计了1对引物,采用RT-PCR技术获得微小牛蜱Bm86基因;将Bm86基因克隆入载体,并进行序列分析,结果证明,克隆的Bm86基因序列与GenBank上登录的Bm86基因序列的同源性达97.4%,而且该序列包含完整的开放阅读框。

Chemical ingredient of the product: Through the essential oil analysis by SRIFFI (short for " Shanghai Research lnstitute of Fragment & Flavor lndustry "), the main ingredient of Ledum palustre oil is as follows:β-pinene 、 p-cymene 、γ-terpineol 、 4-terpineol 、α-terpineol 、 sabinenol 、 nyrtenal 、 bornyl acetate 、 cuninal.

二、产品的化学成份:经上海香料研究所对杜香油的初步分析,发现该油中主要成份有:β-守烯、对-伞花烯、γ-松油醇、 4-松油醇、α-松油醇、桧醇、桃金娘烯醛、乙酸龙脑酯、格茗醛、γ-松油烯-7-醇、乙酸愈创木酯等成份。

Results ①DNA sequencing showed that pBX1 contained a 477bp inserted gene fragment,and when it was compared with the published sequence of the specific region of the gene of the 83kd antigen protein from Borrelia burgdorferi B31 strain,only one amino acid codon was different.

结论该研究成功地对莱姆病螺旋体83kd抗原蛋白特异性区段进行了基因重组和表达,为进一步研究其在莱姆病血清学诊断和基因工程亚单位疫苗研制中的应用奠定了基础。

The surface of the fragment developed a botryoidal or oolitic form.

铁碎片的表面呈现葡萄状或者鲕粒状。

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推荐网络例句

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气,收缩臀部肌肉;拱起身体,尽量抬起头来,右腿伸直朝向天花板(膝微屈,以避免肌肉紧张)。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化,但却比去年同期高8%。

However, to get a true quote, you will need to provide detailed personal and financial information.

然而,要让一个真正的引用,你需要提供详细的个人和财务信息。