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Five pedigrees with high incidence of schizophrenia were studied by polymerize chain reaction and restriction fragment length polymorphism technique.

应用聚合酶链反应和限制性片段长度多态性方法对5个精神分裂症高发家系进行初步研究。

The results showed that Non-haemagglutinating activity was showed in the isolate. In agar diffusion test,there was a clear precipitin band formation to anti-IBDV specific serum. 28-day-old chicks inoculated with the isolate showed disease,and the isolate was recovered from the tissues of these chickens. The 1 041bp specific fragment were amplified by RT-PCR with sequence-specific primers based on IBDV VP3 gene. The VP3 gene shared 97.7%~98.2% nucleotide identities to very virulent IBDV,97.7%~95.2% nucleotide identities with classical strains from seqiemce analysis.

结果表明，该分离病毒无血凝性，在琼脂扩散试验中能与抗IBDV特异性血清出现1条清晰的白色沉淀线；人工感染28日龄雏鸡出现与临床一致的病变，并回收到病毒；应用针对IBDVVP3基因的特异性引物进行RT-PCR，能扩增到长度为1041bp的特异性目的片段；序列分析发现分离毒VP3基因与IBDV超强毒和经典毒株的核苷酸同源性分别为97.7%~98.2%和95.2%。

But Longgupo also yielded a mystery jaw fragment, including the fourth premolar and the first molar.

但龙骨坡还发现了一件神秘的下颌残片，包括第四前臼齿和第一臼齿。

90In this paper, the 10kDa sulfur-rich prolamin gene was amplified by PCR, cloned and sequenced from Chi-nese rice. The sequence analysis showed that the amplified fragment was 380bp long, 94. 5 % homologousto the nucleic acid sequence reported by Masumura et al.

对扩增产物的核苷酸序列分析表明：本扩增产物长 380bp；与 Masumura等人[1] 发表的序列相比，有94.5％的同源性，与我们以前发表的中花10号水稻10kDa富硫醇溶蛋白基因的序列相比，有99.5％的同源性。

Thereafter,DNA fragment of Hyl-1-Amp-Hyl-1 was subcloned into pBR322 vector,the resulting construct was then checked by PCR and restriction analysis for the proper configuration of the knockout vector pBR322-SA.

该载体与质粒pBR322分别用EcoRⅠ、PstⅠ双酶切后进行连接，得到基因敲除的重组载体pBR322SA，PCR 及限制性酶切分析，构建的敲除载体与设计结果相符。

It is better to use a neutron or proton rich projectile with atomic number notfar away from the expected exotic nuclei because the difference in the fragmentationcross section induced by neutron or proton rich and stable projectiles becomes smallwith the increase of the distance between the produced fragment and the projectile. Measurements of 〓 for 〓,〓 and 〓 at intermediate energieshave been performed on the Radioactive Ion Beam Line in Lanzhou.

所选的丰中子或丰质子炮弹最好离所需产生的丰中子或丰质子奇异核比较近，因为随着炮弹与产生的核的距离的增加，丰中子或丰质子炮弹与稳定的炮弹的碎裂反应产生奇异核的产生截面的差别变小。

In order to clone the VIP gene in the gastrointestinal tract from beijing duck, one pair of specific primers to VIP gene was designed and synthesized according to the chick sequence (X80906). Encoding VIP cDNA fragments were amplified by RT-PCR from the total RNA in the Proventriculus, the Duodenum and the Jejunum of Beijing duck. Their PCR products were ligated into pGEM-T easy vector, which was transformed into E. coil JM109. Positive bacteria clones were screened and identified by PCR method and digested with the double restriction enzyme EcoRⅠ. The sequence of VIP gene fragment was also determined and analyzed.

为从北京鸭胃肠道中扩增血管活性肠肽基因，根据鸡VIP基因（GenBank登录号X80906），设计了一对简并引物，从北京鸭腺胃、十二指肠和空肠提取总RNA，通过反转录－聚合酶链反应扩增，将从腺胃、十二指肠和空肠中扩增出的产物克隆到pGEM-Teasy载体上，导入大肠杆菌JM109，阳性克隆经双酶切鉴定后测序，将测序结果与鸡和鹅（GenBank登录号为DQ023161）的VIP基因进行同源性比较。

Methods 112 blood samples of occupationally lead-exposed workers were selected from a puddling factory in the suburb of Chengdu. An assay based on the polymerase chain reaction-restriction fragment length polymorphism technique of restriction enzyme Bsm Ⅰ was used to detect at the same time.

整群抽取成都市郊区某炼铁厂接铅作业人员112人，抽取其静脉血进行血铅、血清钙和基因多态性测定，按基因型测定结果分组分别比较其血铅、血清钙的差异，并进行血铅和血清钙的相关性分析。

Results showed that the PMC meiosis of autotetraploid was similar to the diploid except some particularities. In metaphase Ⅰ, multivalent, quadrivalent, trivalent, bivalent and univalent were observed and some of the chromosomes were found not to rank on the metaphase plate and so did in metaphase Ⅱ cells. In anaphase Ⅰ and anaphase Ⅱ cells, there were lagging chromosomes, chromosome bridge and fragment. During anaphase Ⅱ and telophase Ⅱ, chromosome segregation was not synchronous or equal. At tetrad stage, dyad, triad, tetrad with micronucleus and polyad appeared.

结果表明，同源四倍体花粉母细胞减数分裂过程与二倍体相比，中期Ⅰ染色体构型复杂，有多价体、四价体、三价体、二价体和单价体；中期Ⅰ及中期Ⅱ有部分染色体没有排列在赤道板上；后期Ⅰ及后期Ⅱ出现落后染色体、染色体桥及断片；后期Ⅱ和末期Ⅱ有染色体不同步分离及不等分裂的现象；四分体时期还出现二分体、三分体、含微核的异常四分体及多分体。

As it does so it will initially fragment. It's no longer being fed energy by the seas, and at the moment overcast / drizzle is expected sometime Friday afternoon. That rainband then appears to clear south of Japan.

当该降雨带登陆后就不能从海面上汲取能量了，周五下午估计就会有细雨，日本南部将会出现明显的降雨过程。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。