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By adding a sequence encoding a small peptide,〓, to the base sequence of pYZFcd20, a new phasmid pYZcpp3 was constructed. Using the pYZcpp3 to transform E. coli. 16c9, the genetically engineered bacteria 10916# was obtained. 10916# can secret the soluble chimeric antibody fragment anti-CD20 F〓 into periplasmic.

通过对质粒pYZFcd20的碱基序列的改造，在CH1基因的末端添加了一截二聚化结构的编码序列，实现了在大肠杆菌中直接表达可溶性抗CD20嵌和抗体片段F〓的目的。

Only a small fragment of his annals has been discovered, relating to his invasion of Egypt in 567 BC, and referring to "Phut of the Ionians".

他的编年史仅有一些碎片被发现，讲叙他在公元前567年入侵埃及，也提及"爱奥尼亚的砰"。

To No. 20 are respectively black mudstone, black mudstone ( Caitun mining area of Benxi Basin,Paleozoic), dark grey gangue (Caitun mining area of Benxi Basin, Paleozoic), brown shale(Caitun mining area of Benxi Basin, Paleozoic), brown grey shale(The West Opencut Coal Mine of Fushun Basin, Cenozoic), grey silty mudstone(Haizhou Opencut Coal Mine of Fuxin Basin, Mesozoic), black grey gangue(Haizhou Opencut Coal Mine of Fuxin Basin, Mesozoic), grey yellow clay(Jianping County, Chaoyang City, its era to be determined), grey silicon slate( Qianshan Mountain of Anshan City, pre-Paleozoic), grey phyllite(Qianshan Mountain of Anshan City, pre-Paleozoic), granite-gneiss(Qianshan Mountain of Anshan City, pre-Paleozoic), granite(Mount Yiwulu, its era to be determined), diorite(Mount Yiwulu, its era to be determined), black basalt(The West Opencut Coal Mine of Fushun Basin, Cenozoic), dark purple andesite( Mount Guiyunhua of Zhuanghe City, Mesozoic), fragment of brick-tile-pottery-porcelain( from the building site of Panjin City), coal cinder( from the boiler room of Heji Road of Panjin City), cement( from the building site of Panjin City) and slag (from Bohai Stockroom of Liaohe Oilfield produced in Anshan City).

2-20号样品分别是黑色泥岩、黑色泥岩、深灰色煤矸石、褐色页岩、褐灰色油页岩、灰色粉砂质泥岩(阜新海州露天矿、中生代)、黑灰色煤矸石(阜新海州露天矿、中生代)、灰黄色膨润土、灰色硅质板岩、灰色千枚岩、花岗片麻岩、花岗岩、闪长岩、黑色玄武岩、暗紫色安山岩、砖瓦陶瓷碎片、粉煤灰、水泥、矿渣(鞍山产、取自辽河油田渤海库)，这19个样品用来代表工业、采矿、冶炼、建筑等行业产生的固体废弃物。

Initial sequence data for Pilchard herpesirus was acquired by comparing the highly consered region of the ORF 62 gene of other piscine herpesiruses (Ictalurid herpesirus 1 and Salmonid herpesirus 2 [Oncorhynchus masou irus, SaH2, OM]), and designing primers that successfully amplified a fragment of PH.

扩增产物的序列分析不同于其它已知的疱疹病毒，因而也可以用于区别沙丁鱼疱疹病毒

Initial sequence data for Pilchard herpesvirus was acquired by comparing the highly conserved region of the ORF 62 gene of other piscine herpesviruses (Ictalurid herpesvirus 1 and Salmonid herpesvirus 2 [Oncorhynchus masou virus, SaHV2, OMV]), and designing primers that successfully amplified a fragment of PHV.

扩增产物的序列分析不同于其它已知的疱疹病毒，因而也可以用于区别沙丁鱼疱疹病毒文题：Molecular biology of channel catfish virus

Quantifying tibial plafond fracture severity: absorbed energy and fragment displacement agree with clinical rank ordering.

胫骨远端骨折严重度的量化：吸收能和骨片移位与临床分级次序一致_精华文摘译讯_Medlive医脉通，www.medlive.cn

If the fuel is unable supplies promptly, very much has the possibility to explode by this planetoid after the fragment to hit, because all airships already did not have the enough power to carry on by that time dodge and retreated!

如果燃料无法及时供应的话，就很有可能被这颗小行星爆炸后的碎片击中，因为到那时所有飞船已经没有足够的动力进行规避和撤退了！

Please plotting the logarithm of the DNA fragment sizes versus the migration distances on the plotting paper below, and calculate the sizes of the DNA fragments.

请以DNA片段大小的对数对移动距离作图於所附的作标图中，并计算DNA片段的大小。

The total RNA was isolated from pokeweed leaves using the method of guanidine isothiocyante and used as template for amplification of the deleted mutant pokeweed antiviral protein gene by RT-PCR and then the amplified fragment was cloned into secreted expression pPIC9K vector to form pPIC9K-p, then the vector was transferred into Pachia pastoris GS115 strain.

从美洲商陆叶片中通过异硫氰酸胍法提取出了总RNA，经RT-PCR扩增出缺失突变型抗病毒蛋白PAP基因，将该基因克隆于分泌型真核表达载体pPIC9K，然后导入毕赤酵母菌株GS115细胞。

By using PCR method, a 1kb fragment was gained from Heliothis amigera nuclear polyhedrosis virus genome.

用PCR方法从棉铃虫核多角体病毒基因组中扩增到了1kb的DNA片段，用双脱氧链终止银染测序法测定了该片段的全序列，发现它含有一个完整。。。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。